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Explain Isoelectric pH
Isoelectric pH: Many ionizable groups are present in a protein molecule. Depending on the pH of the medium, some of these groups act as proton donors and some others act as proton acceptors. Thus, proteins are amphoteric compounds. At a specific pH, the protein exists as a dipolar ion (one positive and one negative ion) or zwitterion. So, at this pH the net charge of the protein - becomes zero. This pH is known as isoelectricpHorpI of the protein. Having no net charge, protein does not move to either electrode in an electric field.
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