Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
The plasmids below show the insertion of the EZH2 gene into the pBluescript plasmid in either sense (forward) or antisense (reverse) orientation. These are the plasmids you will hopefully have at the end of your cloning practical. NB - C is the cloning enzyme you used (i.e. EcoRI) and HindIII (indicated as B on the image) is the enzyme you will use to test for inserts.
a) Draw a gel image with the pattern of bands you would expect to see after digesting with the 'forward' or 'reverse' plasmids with HindIII
b) List the exact sizes of the fragments for each of the digests in a)
c) Insertion of EZH2 into the pBluescript plasmid can be selected for on the basis of blue-white selection. Explain how blue-white selection works.
Limiting Factor - Ecosystem In all ecosystems one factor, usually abiotic, limits the growth of organisms and is therefore called a limiting factor. The limiting factor is one
Maedi/visna Maedi, a progressive pneumonia, and visna, a neurotropic disease, found in sheep and goats are caused by the Maedi/visna virus, belonging to the family Retroviridae
Which of the vertebrate classes (a) are 'warm-blooded', (b) reproduce by laying eggs? (a) Birds and mammals are 'warm-blooded'. (b) Fish, Amphibia, Reptiles
Body temperature is homeostatic ally regulated around a set point. Given your knowledge of negative feedback and homeostatic control system, predict whether narrowing or widening o
Development of Fruit Concurrent with the development of the seed(s), the ovary is transformed into a fruit. The fruit protects the seeds and allows their release or germinati
Graphs are a way to convey information and trends in a convenient fashion. For this week's Discussion Board you should search the internet for a graph related to biology. Provide u
An mRNA molecule codifies only one type of protein? Eukaryotic cells have monocistronic mRNA, i.e., every mRNA codifies only one polypeptide chain. Prokaryotes can present poly
VERTEBR A T A It is divided into two parts - 1. Agnetha 2. Gnetha S.No. C haracter A gnetha G
Explain Fetal Development? The medical profession divides pregnancy into three-month stages called trimesters. At the end of the first trimester, the fetus is only about 5 cm l
Determine what the abnormal levels of AFP signal Abnormal levels of AFP may signal the following: Open neural tube Defect (ONTD) Down syndrome Other chromosoma1
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd