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The plasmids below show the insertion of the EZH2 gene into the pBluescript plasmid in either sense (forward) or antisense (reverse) orientation. These are the plasmids you will hopefully have at the end of your cloning practical. NB - C is the cloning enzyme you used (i.e. EcoRI) and HindIII (indicated as B on the image) is the enzyme you will use to test for inserts.
a) Draw a gel image with the pattern of bands you would expect to see after digesting with the 'forward' or 'reverse' plasmids with HindIII
b) List the exact sizes of the fragments for each of the digests in a)
c) Insertion of EZH2 into the pBluescript plasmid can be selected for on the basis of blue-white selection. Explain how blue-white selection works.
Describe what would happen if people lost all of their mitochondria and explain why it would happen.
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