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The plasmids below show the insertion of the EZH2 gene into the pBluescript plasmid in either sense (forward) or antisense (reverse) orientation. These are the plasmids you will hopefully have at the end of your cloning practical. NB - C is the cloning enzyme you used (i.e. EcoRI) and HindIII (indicated as B on the image) is the enzyme you will use to test for inserts.
a) Draw a gel image with the pattern of bands you would expect to see after digesting with the 'forward' or 'reverse' plasmids with HindIII
b) List the exact sizes of the fragments for each of the digests in a)
c) Insertion of EZH2 into the pBluescript plasmid can be selected for on the basis of blue-white selection. Explain how blue-white selection works.
Pikas (component 1), also known as "rock rabbits" or "coneys", are small relatives of rabbits belonging to the genus Ochotona. The 30 species in this genus are confined to high alt
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What are brief descriptions of how you could test hypothesis. or how would you know if your hypothesis is testable?
Biopathways 1. Find out pathway information about these proteins using the Human Reactome. O00327 O14503 O75586 O95096 P01137 P01308 P06744 P08240 P12
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