Explain how blue white selection works, Biology

Assignment Help:

The plasmids below show the insertion of the EZH2 gene into the pBluescript plasmid in either sense (forward) or antisense (reverse) orientation. These are the plasmids you will hopefully have at the end of your cloning practical. NB - C is the cloning enzyme you used (i.e. EcoRI) and HindIII (indicated as B on the image) is the enzyme you will use to test for inserts.

2462_Explain how blue-white selection works.png

a) Draw a gel image with the pattern of bands you would expect to see after digesting with the 'forward' or 'reverse' plasmids with HindIII 

b) List the exact sizes of the fragments for each of the digests in a) 

c) Insertion of EZH2 into the pBluescript plasmid can be selected for on the basis of blue-white selection. Explain how blue-white selection works.


Related Discussions:- Explain how blue white selection works

Express the term - aboral surface, In radially symmetric animals there is n...

In radially symmetric animals there is no posterior, or anterior, right sides to the animal. Rather we refer to two sides of the animal by referring to the location of the mouth. I

What are the three major types of passive transport, Q. What are the three ...

Q. What are the three major types of passive transport? The three main types of passive transport are simple osmosis, diffusion and facilitated diffusion.

What is smooth muscle, What is Smooth Muscle? Smooth muscle provides th...

What is Smooth Muscle? Smooth muscle provides the contractile force for movement in internal organs under control of the involuntary or autonomic nervous system. Smooth muscle

Explain the types of basic forces, Q. Explain the types of basic forces? ...

Q. Explain the types of basic forces? Compression: This refers to the squeezing of the test material so that it still remains as a single undivided unit but may occupy less v

Explain the dimensions of practice of nutrition care process, The nutrition...

The nutrition care process acknowledges the common dimensions of practice by  the following: Defining a common language that allows nutrition practice to be more measurable,

Define the concepts of lipids, Define the Concepts of Lipids? You have ...

Define the Concepts of Lipids? You have studied the classification of lipids in your theory lesson. Lipids, you learnt, are classified as simple, compound or derived lipids on

Synthesis of polyamine, Synthesis of Polyamine As the name of this cla...

Synthesis of Polyamine As the name of this class of compounds suggests, polyamines have several amino groups replacing hydrogen usually in alkyl chain e.g., putrescine is 1, 4

Describe the two pieces of evidence, Describe two pieces of evidence indica...

Describe two pieces of evidence indicating that speciation does not always occur at the same rate. Some species of moths on the Hawaiian Islands appear to have evolved during t

Diaphragmatic hernia, Diaphragmatic Hernia: In this condition there  i...

Diaphragmatic Hernia: In this condition there  is a slight herniation ofabdominal organs  (stomach intestine and liver) or extreme protrusion of abdominal contents into the th

If jean-baptiste de lamarck was correct so result will be, If jean-Baptiste...

If jean-Baptiste de Lamarck was correct, than a. Natural selection would lead to the spread of favourable traits within a population b. Finches that stretched their beaks to access

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd