Explain how blue white selection works, Biology

Assignment Help:

The plasmids below show the insertion of the EZH2 gene into the pBluescript plasmid in either sense (forward) or antisense (reverse) orientation. These are the plasmids you will hopefully have at the end of your cloning practical. NB - C is the cloning enzyme you used (i.e. EcoRI) and HindIII (indicated as B on the image) is the enzyme you will use to test for inserts.

2462_Explain how blue-white selection works.png

a) Draw a gel image with the pattern of bands you would expect to see after digesting with the 'forward' or 'reverse' plasmids with HindIII 

b) List the exact sizes of the fragments for each of the digests in a) 

c) Insertion of EZH2 into the pBluescript plasmid can be selected for on the basis of blue-white selection. Explain how blue-white selection works.


Related Discussions:- Explain how blue white selection works

How can we control to occur campylobacteriosis diseases, How can we control...

How can we control to occur Campylobacteriosis diseases If a person is suffering from campylobacteriosis, he can take an antibiotic such as ciproflaxin or azithromycin. Erythro

What is a burette and how is it used, What is a burette and how is it used?...

What is a burette and how is it used? A burette is a uniform-bore glass tube with fine gradations and a stopcock at the bottom, used particularly in laboratory procedures for a

Homework, How do adhesion and cohesion help plants move materials?

How do adhesion and cohesion help plants move materials?

Explain strengthening the public health measures - anaemia, Explain Strengt...

Explain Strengthening the public health measures? Parasitic infestations and protozoal infections cause iron deficiency through loss of blood or destruction of red blood cells.

Explain the endocrine glands involved in the menstrual cycle, What are the ...

What are the endocrine glands involved in the menstrual cycle? What are the hormones in action? The endocrine glands that secrete hormones included in the menstrual cycle are t

Streptococcal antibody test, In about 80 per cent of ARF patients, ASO titr...

In about 80 per cent of ARF patients, ASO titre is significantly raised. ASO titres vary with age, geographical area and other fevers, which influence frequency of streptococcal in

Heat exchangers-type of oxygenators , Heat Exchangers :  This is an integr...

Heat Exchangers :  This is an integral part of cardio pulmonary bypass and is designed to cool and warm the perfusate. non-sterile water from ice bath or warm water passed through

Set up a potometer in the laboratory, A student set up a potometer in the l...

A student set up a potometer in the laboratory and measured the rate of movement of water in the capillary. An average of four readings gave a rate of 50mm per minute. The apparatu

Relationship between the ovulation and menstrual cycle, Q. What is the rela...

Q. What is the relationship between the ovulation and menstrual cycle? Ovulation is the releasing of the female gamete from the ovary and Ovulation is a periodical event that o

Simple proteins, SIMPL E PROTEINS The proteins are made of amino acids...

SIMPL E PROTEINS The proteins are made of amino acids only. Additional chemicals are absent. These are of two types -    (i) Fibrous        (ii) Globular ( i )

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd