Explain disadvantage of colonies at different dilution, Biology

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Explain the Disadvantages of Colonies Obtained At Different Dilution

1. Heat sensitive microorganisms may be damaged by melted agar, giving low viable count as compared to spread plate.

2. Appearance of colonies on differential media cannot be used satisfactorily for diagnostic purpose, if it is growing within the agar. To accomplish this, spread plate method can be used.

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