Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Define the need for classification of plants and animals, Define the Need f...

Define the Need for classification of Plants and Animals? First of all there is a need to know what classification is? Let us define in simple term. Classification is placing o

Cerebellum, why cerebellum more developed in mammals that jump or fly?

why cerebellum more developed in mammals that jump or fly?

Biochemistry, help me in writing assignment on racemization,mutarotation.

help me in writing assignment on racemization,mutarotation.

Calcium control of glycogen metabolism, As we have seen, in during epinephr...

As we have seen, in during epinephrine hormonal control or glucagon, dephosphorylated   phosphorylase   kinase   is activated by being phosphorylated by protein kinase. This then a

Cost of accidents, Cost of Accidents Immediate Cost Immediate c...

Cost of Accidents Immediate Cost Immediate cost takes into account the loss of work, damages to equipment and facilities which have to repaired and restored or replaced

Can you explaon about aortography, Q. Can you explaon about Aortography? ...

Q. Can you explaon about Aortography? Visualization of the aorta and its branches is possible by several modalities today. Apart from angiography, aorta can also be visualized

Why do protease-supplying cells of the stomach, Q Why do protease-supplying...

Q Why do protease-supplying cells of the stomach and of the pancreas make only precursors of the active proteolytic enzymes? The stomach and the pancreas make zymogens of the p

Define pipette - nutritional biochemistry, Define Pipette - Nutritional Bio...

Define Pipette - Nutritional Biochemistry? Pipettes are used to deliver a known volume of liquid from one container to another. They are precision instruments of measurement an

Define structural elements - biological functions of protein, Define Struct...

Define Structural Elements - Biological Functions of Proteins? Structural proteins are fibrous proteins. The well known of the fibrous proteins are possibly the keratins, which

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd