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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
Explain about the Derived Proteins? These are not naturally taking place proteins and are obtained from simple proteins by the action of enzymes and chemical agents, heat, mech
What do you understand by Heterotrophic? Organisms which are not capable of converting light into chemical energy. They should consume other organisms or material produced by o
How can proteins be classified? Give suitable examples. Proteins can be classified based on: Shape and size: fibrous or globular proteins. Functional properties: I
Carbohydrates These form about 1%part of protoplasm, they are comparatively simpler compounds of carbon, hydrogen and oxygen in the ratio 1:2:1 The ratio hydrogen to oxygen is
Photoperiodism was first characterised in : 1. Tobacco 2. Potato 3. Tomato 4. Cotton Tobacco is the first in whic photoperiodism characterised.
Monosaccharides :- Sugars consisting of a single polyhydroxy aldehyde or ketone unit.
Explain about the Microcolony DEFT? It allows determination of viable cells. Food homogenate is filtered through a DEFT membrane and then placed on the surface of appropriate c
Define Requirements and Recommended Dietary Allowances (RDA)? What do we mean by requirements and RDA? The requirement level is the amount of nutrient needed to be absorbed to
The plasma membrane consists mainly of: 1. Phospholipids embedded in a protein bilayer 2. Proteins embedded in a phospholipid bilayer 3. Proteins embedded in a polymer of
The difference b/n algae,bryophyte&pteridophyte?
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