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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
1. Write general characters of phylum mollusca and classify it upto classes, giving at least two examples of each class.
What is Polymerase Chain Reactionin genetics? A great advance in sequencing and cloning DNA is the polymerase chain reaction, devised by the American biochemist Kary Mullis in
ARE BONY FISHES AMMONOTELIC?!
Osmoregulation in Terrestrial Environment Earlier you have learnt about osmoregulation in aquatic environment. In this section, we shall study how the terrestrial animals cop
SKIN - Largest organ of body. It is covered by hairs or pelage i.e. pillifera. Study of skin is dermatology. Outermost covering of body. Skin is elastic and
Must account for both food AND light as zeitgebers Because of the continuous light / dark periods of the year; light not always able to act as a zeitgeber. Food
Sally Subcloner wants to clone a fragment into pUC19 (Map above – From New England Biolabs). Her fragment was a protein coding gene of 2.7 kb, and had XhoI sites on either end (and
While studying evolution, a student comes across a cladogram that includes clades like amphibia, reptilia, aves, and mammalia. What must be the basal clade?
Define Amino Acids in the Building Blocks of Proteins? Amino acids, as you have already illustrated, have a carboxyl group and an amino group bonded to a common carbon atom and
Explain the Position of the endospore? Endospore cannot be stained by ordinary methods such as simple staining and gram staining because these dyes do not penetrate the wall of
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