Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Quality control of feedstuffs, Quality control of feedstuffs Quality co...

Quality control of feedstuffs Quality control is essential at all stages in the production of compound feed so that animals could get wholesome balanced and nutritious feed. Th

Explain the primary treatment for managing food allergies, Explain the Prim...

Explain the Primary Treatment for Managing Food Allergies? The primary treatment for managing food allergies is eliminating the offending food or foods. In fact, non-pharmacolo

Morphogenesis of an ectodermal derivative, Morphogenesis of an Ectodermal D...

Morphogenesis of an Ectodermal Derivative During the development of vertebrate body the dissimilar regions in the three germ layers of the gastrula become segregated from each

Define starch gelatinisation, Starch Gelatinisation Starch is insolubl...

Starch Gelatinisation Starch is insoluble in cold water but in warm water it swells until its gelatinization temperature begins to lose its structure and leaches out its  8con

Viruses, are viruses cellular organisms

are viruses cellular organisms

What are varices, What are varices? Why are they more common in the inferio...

What are varices? Why are they more common in the inferior limbs? Varix means abnormal enlargement of veins. Varices happen when excessive pressure against the normal blood fl

Programmes directed towards the common crop plants, Programmes directed tow...

Programmes directed towards the common crop plants These programmes can be directed towards incorporating the following traits among common crop plants. Non-photo

Brasseler endo extractor tube-endodontics principles, Brasseler Endo Extrac...

Brasseler Endo Extractor tube Insert H file buccally and lingually and remove silver by friction.If I can't remove use solvent to desolve cement If the tip of silver not appear

Determine the three types of joints, Determine the three types of joints ...

Determine the three types of joints There are three types of joints : Fixed or immovable or fibrous : There is no space between the bones. The attached bones are very ti

The key enzymes of gluconeogenesis, The key enzymes of gluconeogenesis,incl...

The key enzymes of gluconeogenesis,include: a)  Pyruvate carboxylase b)  Phosphoenol pyruvate carboxykinase c)  Fructose- 1,6-bisphosphatase,  and d)  Glucose-6-phosph

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd