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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
At the starting of the first round of elongation, the initiation codon (AUG) is positioned in the P site with fMet-tRNAfMet bound to it by codon-anticodon base pairing. In The nex
'Absolute food safety' is the assurance that damage or injury from use of a substance is impossible. Normal 0 false false false EN-US X-NON
explain briefly about ICZN
A home-made wormery A wooden box 30 cm by 30 cm by 15 cm fitted with a glass front is beneficial for studying the habits of earth-worms. Fill the box nearly to the top with
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I have 30µg of a primer, whose molecular weight is 600. How much water should I add to get a stock primer concentration of 100µM?
Evaluation This is the last step in nursing process. Throughout care, you constantly evaluate the progress the client has made towards reaching the pre-established goals/ ob
Q. Grading of Stenosis and Grading of Coronary Artery Disease? Multiple views are necessary to quantify coronary stenosis accurately. Further, there should be no foreshortening
What are heterochromatin and euchromatin? Chromatin is uncondensed nuclear DNA, the typical DNA morphology in interphase (the phase of the cell cycle in which the cells is not
With the help of reaction briefly explain the oxidative phase of the pentose phosphate pathway. The oxidative branch of the pathway generates NADPH and pentose-5 phosphate, th
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