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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
Vitamin B 1 (Thiamine hydrochloride) a) Thiamine hydrochloride is a white, crystalline powder, slightly hygroscopic, of an odour resembling yeast and of bitter to taste. b)
classification and characteristics
what is anotmy
Define Criteria for Assessment of Pyridoxine Status? Vitamin B 6 status is most appropriately evaluated by using a combination of indicators, namely plasma PLP concentration,
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What proportion of children with Down syndrome do you expect when women with down syndrome have children with men who have 46 chromosomes Justify your answer
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