Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Extracted chlorophyll pigments, Extracted chlorophyll pigments If we...

Extracted chlorophyll pigments If we take chlorophyll molecule in a test tube and provide 2 CO and water and expose the mixture to sunlight, glucose is not formed. If we

What do you mean by somites, Q. What do you mean by somites? Somites ar...

Q. What do you mean by somites? Somites are differentiated portions of mesodermal tissue longitudinally distributed along the embryo and the somites originate the muscle portio

Diseases caused by viruses, Diseases Caused by Viruses In the early day...

Diseases Caused by Viruses In the early days of microbiology, the disease producing submicroscopic agents were termed as 'filterable viruses' because of their ability to pass t

State the principle of adaptation, State the Principle of Adaptation Th...

State the Principle of Adaptation The first principle is that the central goal of assessment is to promote the adaptation of the child, rather than simply to document the prese

Describe the term humidification, Describe the term Humidification Dry...

Describe the term Humidification Dry gases result in the loss of ciliary activity of the mucosa of the airway and can lead to mucosal damage as well as causing the secretions

Explain tertiary butylhydroquinone, Tertiary Butylhydroquinone (TBHQ) T...

Tertiary Butylhydroquinone (TBHQ) TBHQ is moderately soluble in oil and slightly soluble in water. In many cases, TBHQ is more effective than any other antioxidant in providing

What is the incubation period of an infection, What is the incubation perio...

What is the incubation period of an infection? Incubation period is the time interval among the infection by an agent that causes disease and the first signs or symptoms of th

Pulp tissue revascularization, Pulp Tissue Revascularization :   Defi...

Pulp Tissue Revascularization :   Definition: Is the procedure to re-establish the vitality in non-vital tooth by allowing repair & regeneration of the tissues.

Echinoderm - circulatory and nervous system, Q. Echinoderm identity card. H...

Q. Echinoderm identity card. How are echinoderms characterized according to examples of representing beings, basic morphology, type of symmetry, germ layers and coelom, respiratory

Define term population, What is a population? In Biology a population i...

What is a population? In Biology a population is a set of individuals of the similar species living in a given place and in a given time.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd