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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
Explain the Composition of Nutrient Agar and Nutrient Broth? The composition and procedure for preparation of some commonly used media, which we will use in the laboratory for
Lipolysis What do we mean by lipolysis? Hydrolysis of ester bonds in lipids is called lipolysis. This may occur by enzyme action or by heat and moisture, resulting in the liber
This valve has the same basic features as of the Tricuspid Valve. It has an anterior and a posterior cusp. The anterior cusp is larger and is attached on the upper right part of t
A pp l i c a tion s of transgenic animals Current applications of gene transfer in farm animals include the improvement of product quality and quantity, disease resistan
1. Define each term and give an example of an application that uses the following: a. Biocompatible material b. Biodegradable material c. Biomimetic
Which of the following serves as an effector, or as part of an effector, that functions in a negative feedback system? A. Nicotinic Acetylcholine Receptors (nAChR) in SA node c
Types of polyembryony
Behavioural Adjustments When faced with a sudden temperature change, most animals make behavioural responses that enable them to avoid extreme or lethal conditions. Among the
Water is most dense and thus heavier at 4 degreese celceus. At 0 degreese celceus ice forms and can float on liquid water. Assuming that ice were most dense at 0 degreese celceus,
Hemerythrins - Respiratory Pigments The Hemerythrins are rather rare. They take place in some animals belonging to the minor phyla like the sipunculid worms, some brachiopods,
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