Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Non-extractive use values, Some activities that make use of biological reso...

Some activities that make use of biological resources, without involving their extraction or destruction, denote non- extractive use of biological resources. This includes ecotouri

What is pcr, What is PCR? How does PCR works? PCR, polymerase chain rea...

What is PCR? How does PCR works? PCR, polymerase chain reaction, is a process to synthesize many copies of exact regions of a DNA molecule known as target-regions. Its inventor

Mode of nutition, What is the mode of nutrition of Ascaris?

What is the mode of nutrition of Ascaris?

Pulmonary arterial system changes, Q. Pulmonary Arterial System Changes ? ...

Q. Pulmonary Arterial System Changes ? a) Pulmonary Plethora With increased pulmonary arterial blood flow, pulmonary branches are visualized beyond the inner 2/3 of the lung

Diagrammatic representation of implant placement, Diagrammatic representati...

Diagrammatic representation of Implant placement Implant placementIt is located directly apical to the tooth it is replacing and not in an embrasure space. The angulati

Explain elastase, Elastase The inactive  proelastase  is activated by t...

Elastase The inactive  proelastase  is activated by trypsin to the active form elastase. Elastase attacks  peptide  bonds  next  to  the  small amino  acid residues such  3s  g

Preparation of dilutions - standard or total plate counts, Define Preparati...

Define Preparation of Dilutions - Standard or Total Plate Counts? 1. Serial dilution of homogenized sample in an appropriate diluent. Preparation of dilutions - Decimal dilu

How to compete for space and nutrients with pathogen, How do normal flora p...

How do normal flora protect against infection? a. Compete for space and nutrients with pathogens b. Release antimicrobial agents to kill pathogens c. Contribute to host health by p

Define some example of acidic and basic buffer, Define Some Example of Acid...

Define Some Example of Acidic and Basic Buffer? Frequently used example of acidic and basic buffer includes: - Acidic buffer: a mixture of CH 3 COOH (acetic acid) + CH 3 C

Egg type, why their is different amount of yolk is present in eggs.

why their is different amount of yolk is present in eggs.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd