Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Skeletal muscle need an antagonistic partner, Why does each skeletal muscle...

Why does each skeletal muscle need an antagonistic partner? Because muscles can only contract and relax, each skeletal muscle requires an antagonistic partner to extend it afte

What is the adnexal organ of the digestive system, Q. What is the adnexal o...

Q. What is the adnexal organ of the digestive system in which bile is stored? How does this organ react to the ingestion of fat rich food? Bile is concentrated and stored in th

5 kingdom system, what r the demerits of 5 kingdom classification?

what r the demerits of 5 kingdom classification?

Determine the food sources of vitamin a, Determine the Food Sources of Vita...

Determine the Food Sources of Vitamin A? Vitamin A or retinol performed vitamin A), as you may already know, is found only in foods of animal origin, such as milk, cheese, crea

What is the commercial importance of algae, Q. What is the commercial impor...

Q. What is the commercial importance of algae? Numerous algae have high nutritional value and are commercialized and consumed as human food they are very popular food in the or

Examine the cysticerus larva under the microscope, Examine the cysticerus l...

Examine the cysticerus larva under the microscope? Examine the cysticerus larva under the microscope and note the following: i)  Bladder worm consists of a single layered, l

Pericardial valves: carpentier edwards, Pericardial Valves: Carpentier Edwa...

Pericardial Valves: Carpentier Edwards (Perimount Valve) :  Pericardium preserved in gluteraldehyde and mounted on flexible stent is available for mitral and aortic positions

What are the three parts of a dna nucleotide, What are the three parts of a...

What are the three parts of a DNA nucleotide, and how are they connected to each other? The three types are a deoxyribose sugar, a phosphate group, and a nitrogenous base. The

Initiation of development - development biology, Initiation of Development ...

Initiation of Development - Development Biology We know that the various events collectively called the activation programme. These events are directly related to the formatio

What is acute aortic regurgitation, Q. What is Acute Aortic Regurgitation? ...

Q. What is Acute Aortic Regurgitation? Infective endocarditis, aortic dissection and trauma often produce severe AR. Acute increase in left ventricular end diastolic volume es

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd