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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
what are the sub- headings in energy?describe energy in biology?
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Sulphur Sulphur is a component of the animo acids methionine, cysteine and cystine; the B-vitamins, thiamin and biotin; as well as a number of the organic compounds. Sulphate,
General Characters-
In E. coli there are seven rRNA transcription units scattered by the genome, each of that contains one copy of every of the 16S, 23S and 5S rRNA genes and one to four c
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