Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Zoology, why protozoass dont have vaccines esp. plasmodiums

why protozoass dont have vaccines esp. plasmodiums

Why is it necessary to maintain the ph level, Q Coming from the acid pH of ...

Q Coming from the acid pH of the stomach which pH level does the chyme find when it enters the duodenum? Why is it necessary to maintain that pH level in the small intestine? What

Define standard titration for estimation of reducing sugar, Define Standard...

Define Standard Titration for estimation of reducing sugar by Fehling Soxhlet Method? Pipette 5 ml of Fehling's A and 5 ml of Fehling B solution into a 100 ml conical flask. Ad

Define fat requirements in human body, Define Fat requirements in human bod...

Define Fat requirements in human body? There is no change in fat digestibility at altitude of

Organic chemistry and macromolecules, identify 7 speccific ways in which yo...

identify 7 speccific ways in which you can diversify carbon-containing compounds

Define denaturation, Denaturation: With respect to the nucleic acids, refe...

Denaturation: With respect to the nucleic acids, refers to the conversion from the double-stranded to single-stranded state, many times achieved by heating or alkaline conditions.

Metalimnion - summer stratification, Metalimnion - Summer Stratification ...

Metalimnion - Summer Stratification This zone lies below the epilimnion and above the hypolimnion and thus forms the intermediate layer which is non-circulating. The metalimni

Membrane that selects based on pore size, A transport process in which phys...

A transport process in which physical (hydrostatic) pressure drives part of a fluid through a membrane that selects based on pore size

Fossil history of horse, Evolution of Horse - Credit to Prof. C. Marsh...

Evolution of Horse - Credit to Prof. C. Marsh (1879) of Yale University for complete work.   S.No   Fo ssil/Genus   Period

Microorganisms on on basis of nutrient degradation capacity, Q. Microorgani...

Q. Microorganisms on On basis of nutrient degradation capacity? On basis of nutrient degradation capacity: - Proteolytic: Microorganisms, which are capable of protein degra

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd