Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Pylum mollusca, Economic importance of phylum mollusca

Economic importance of phylum mollusca

Explain the gall bladder disease - effect of obesity, Explain the Gall blad...

Explain the Gall bladder disease - Effect of Obesity? Obesity is one of the risk factors for formation of gallstones. The super saturation of bile with cholesterol in obese ind

Pulmonary autograft and ross procedure, Pulmonary Autograft and Ross Proced...

Pulmonary Autograft and Ross Procedure :  Sir Donald Ross in 1967 introduced the concept of using patient's own pulmonary valve (autograft) for aortic valve replacement and a pul

List the major chemical changes occurring in food, Q. List the major chemic...

Q. List the major chemical changes occurring in food? The major chemical changes occurring in food are lipid oxidation leading to rancidity, loss of vitamins and degradation

Into which classes are mollusc divided, Q Into which classes are mollusc di...

Q Into which classes are mollusc divided? What are some representing beings of each class? The phylum Mollusca is divided into five main classes: pelecypods, or bivalves (Pelec

Oxidative phosphorylation occurs in the inner membrane, In  eukaryotes,  th...

In  eukaryotes,  the electron  transport  and oxidative phosphorylation occurs in the inner membrane of mitochondria.  These processes re-oxidize the FADH 2 and NADH   which comes

Phases of viruses, Phases of viruses Viruses have two phases to their ...

Phases of viruses Viruses have two phases to their existence Inside their host cells where they exhibit certain living characteristics. outside their host where they

Can you define musculoskeletal system, Q. Which are the organs that are par...

Q. Which are the organs that are part of the musculoskeletal system? The key tissues and organs that are part of the musculoskeletal system in humans are the cartilages, the mu

Show the stages in which the cell respiration is divided, Q. What are the t...

Q. What are the three stages into which the cell respiration is divided? The three stages of aerobic cell respiration are glycolysis, Krebs cycle and respiratory chain also kno

Describe about coronary reactive hyperemia, Q. Describe about coronary reac...

Q. Describe about coronary reactive hyperemia? Myocardium depends almost completely on aerobic metabolism. Occlusion of a coronary artery even briefly produces an increase in c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd