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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
What are the six criteria used to build a complete evolutionary branch of vertebrates? Dichotomy in each of the six following criteria builds the vertebrate evolutionary branch
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Are protozoan''s diploblastic or triploblastic
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Determine the fate of manganese which is absorbed? Let us now study the fate of Mn which is absorbed. After absorption, Mn is complexed with albumin and transported to the l
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