Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Behaviour change communication, Behavior means responses or reactions or ac...

Behavior means responses or reactions or acting in any situation. Change means act of changing or to become different or to modify. Behaviour Change can refer to any modifica

What are the major factors of colloidal dispersion, What are the major fact...

What are the major factors of colloidal dispersion The adsorption of ions by colloidal particles is a major factor in stabilizing a colloidal dispersion, since the colloidal pa

What is cardiac catheterization, Q. What is Cardiac Catheterization? Ro...

Q. What is Cardiac Catheterization? Routine cardiac catheterization is not necessary in patients with mitral regurgitation. It may be done when there is discrepancy between cli

Neuron, It arises from a one embryonic cell known as neuroblast Types of...

It arises from a one embryonic cell known as neuroblast Types of neuron:  According to number of process¬ (a) Unipolar: Only axon is there. e.g. mesencephalic nucleus. (b)

PHYLUM MOLLUSCA, How we attempt a question of phylum mollusca?

How we attempt a question of phylum mollusca?

Criteria for diagnosis of respiratory failure, Criteria for Diagnosis of Re...

Criteria for Diagnosis of Respiratory Failure PaO 2 , PaCO 2 , > 50 mm Hg.  Vital Capacity Respiration > 30/min or below 8/min.

Transcription and processing of trna in prokaryotes , The rRNA transcriptio...

The rRNA transcription units in E. coli hold some tRNA genes which are processed and transcribed at the time of rRNA transcription. The other tRNA genes  happen  in  clusters  of

Induction of defensive barriers in plants, Induction of Defensive Barriers ...

Induction of Defensive Barriers in Plants Several polymers deposited near surface of plants form an effective barrier against invasion or stress. Cuticle (which consists of a

What is the function of mineral salts, Q. What is the function of mineral s...

Q. What is the function of mineral salts in the creation of electric tension (voltage) at the cellular level? The electric activity of the cell, for example, in neurons, depend

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd