Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

The principal nitrogenous excretory in humans, The principal nitrogenous ex...

The principal nitrogenous excretory compound in humans is synthesised: 1. in kidneys but eliminated mostly through liver 2. in kidneys as well as eliminated by kidneys 3.

Explain the community ecology of disease, Explain the Community ecology of ...

Explain the Community ecology of disease? Ebola, hantavirus, bubonic plague, Lyme disease, West Nile virus, rinderpest, and canine distemper virus are all instances of diseases

Define proteins required for underweight - nutritional care, Define Protein...

Define Proteins required for underweight - Nutritional Care? Proteins are required for tissue building, as well as, to take care of the daily wear and tear. Under weight indivi

Respiration, difference between the respiration occurs in lower and higher ...

difference between the respiration occurs in lower and higher organisms

Discuss about nerve to mylohyoid, Nerve to Mylohyoid Motor branch of i...

Nerve to Mylohyoid Motor branch of inferior dental nerve which descends in a groove on the medial surface of the mandibular ramus. Surgical intervention in this area may lead

Undergraduate thesis title, Hi,I am a graduating student from my course BS ...

Hi,I am a graduating student from my course BS in Biology major in Zoology. I was wondering if you guys have any great idea of a good thesis title?

Periodicity - qualitative characters, Periodicity (Phenology, Aspection) ...

Periodicity (Phenology, Aspection) It refers to the study of seasonal changes in the community, that is, the periodic phenomena of organisms in relation to their climate. Peri

Assessment of ventricular septal defect, Assessment   On assessment a c...

Assessment   On assessment a child with ventricular septal defect will show clinical manifestations depending upon the size of  the defect. The small defects are usually asympt

Describe coping skills, Have you ever said, ‘I cannot cope up with this?' O...

Have you ever said, ‘I cannot cope up with this?' Or have you heard any one who is sick in your family saying, ‘I cannot cope with my sickness' or ‘I can not take care of this sick

What do you mean by hemodialysis, Q. What do you mean by hemodialysis? ...

Q. What do you mean by hemodialysis? Hemodialysis is the artificial blood filtration made by specific machines in alternative of the kidneys. Hemodialysis may be necessary in p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd