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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
Q. What is biodiversity? The Biological diversity is the variety of species of living beings of an ecosystem. In the ecosystems which are more biodiverse, like tropical forests
Explain Precautions for Gram Staining of Bacterial Cultures? 1. The dilutions should be chosen on the basis of expected counts in the food sample. 2. Food should be kept in
Summary of Accident Causation In the light of above theories it is concluded that the decision of workers to work safe is largely influenced by external factors such as meetin
Explain the Indole Production Test? Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can
Q. How can you describe Pleural Effusion? Fluid has a density indistinguishable from soft tissue on a radiograph. Pleural fluid tends to accumulate in the deepest part of the
Follow a cell through the cell cycle. How many copies of each gene are present at each stage?
Three point charges are located at the corners of an equilateral triangle. Find the magnitude and direction of the net electric force on the 1.60 uC charge. (Let A = 1.60 uC, B= 6.
Q. Why is AIDS difficult to prevent by vaccination? It is not easy to produce a vaccine against AIDS because the HIV is a highly mutant virus. In approximately every replicatio
R a d i o g raphic imaging: Radiology has been in use since the discovery of X-rays by Wilhelm Conrad Roentgen in 1895. Until 1980s, radiography was the
Risk management option assessment : Identification of available management options Selection of preferred management option , including consideration of an appropriate saf
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