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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe
What are three basic phases of PCR? Which phase of these three phases is used for analysis for the end-point PCR? Why? Which phase is used for analysis for the quantitative real-t
ISOMERISM AND STERIOISOMERISM IN HEXOSE SUGERS
Q.Explain Pericardial Effusion? The configuration of the heart in pericardial effusion depends on the volume of fluid and its distribution. It may have a globular or non-speci
Define about the Chemotherapy - Cancer? Chemotherapy results in lot of side effects. This is because the drug effects are not specific to cancer cells alone. Even the host cell
Open Type of Circulatory Systems Several non-chordates have thus an open type of circulation - a system in which blood pumped by the heart empties through an artery into an op
Define Protection of poly unsaturated fatty acids (PUFA) from oxidative damage? The major biological role of vitamin E is to protect PUFAs and other components of cell membrane
wrt spoilage
Insects - Hormones in Growth and Reproduction In insects hormones regulate moulting and metamorphosis. The larvae or nymphs which hatch out of the eggs undergo regular moultin
Which terrestrial vertebrate group is extremely rare in deserts? Amphibians are terrestrial vertebrates extremely rare in desert environments (although there are a some species
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