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Explain Counter Stain - Ziehl-Neelsen Method?
Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.
Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.
Zoonotic paramyxoviruses Three zoonotic paramyxoviruses (Hendra, Menangle, and Nipah viruses) emerged during 1994-2004 and all these viruses have a fruit bat reservoir. Humans are
Q. What is structural formula of glycerol? To which organic function do these molecules belong? Glycerol is a linear chain of three carbons, the central carbon is bound to one
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W a s t e Management in other species Solid and pellet nature of sheep and goat faecal matter make it easier to handle. This manure can easily be swept off the floors and d
(a) Find the electric potential, taking zero at infinity, at the upper right corner (the corner without a charge) of the rectangle in the figure. (Let y = 3.7 cm and x = 6.7 cm.)
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Genetics of Nitrogen-fixation The genetics of nitrogen-fixation is known in detail in Klebsiella pneumoniae. There are twenty genes required in organising the complete N 2 -fi
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