Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

What is the role of vitamin k in the blood coagulation, Q. What is the orga...

Q. What is the organ where most of the clotting factors are produced? What is the role of vitamin K in the blood coagulation? Most of the clotting factors are produced in the l

Define dietary management for burns, Define Dietary Management for Burns? ...

Define Dietary Management for Burns? Nutritional support is a major part of therapy for a patient with burns in view of the large catabolic losses, essential anabolic demands a

Limitations of doppler echocardiograph, Though  it has bees suggested that ...

Though  it has bees suggested that Doppler echocardiography may be useful for non-invasive determinations of  stroke volume and cardiac output, it  should be recognized that th4re

Protoplasmic streaming and tubular peristaltic flow model, Protoplasmic Str...

Protoplasmic Streaming and Tubular Peristaltic Flow Model The first of the above two models involves the well known phenomenon of cytoplasmic streaming in giant algal cells. C

How to decreases the rate limiting, When the product of a metabolic pathway...

When the product of a metabolic pathway decreases the rate limiting earlier in the pathway it is called?

Transport and facilitated diffusion have in common, Q. What do active trans...

Q. What do active transport and facilitated diffusion have in common? What are the dissimilarity between them? Facilitated diffusion can be perplexed with active transport beca

Taxonomy.., what is the androcium in china rose ..

what is the androcium in china rose ..

Explain chronic infections fever, Explain Chronic Infections Fever Chr...

Explain Chronic Infections Fever Chronic  Infections Fever: These are generally of longer and sustained duration. The patients have a past history of repeated episodes or con

Headache associated with neurological diseases, Headache Associated with Ne...

Headache Associated with Neurological Diseases Headache is a symptom of many nervous system disorders, usually resulting from the distortion of pain sensitive structures. Commo

Explain the health benefits of polyphenols, Explain the Health Benefits of ...

Explain the Health Benefits of Polyphenols ? Various health benefits have been attributed to polyphenols. These include: - anti-microbial - anti-viral - anti-oxidant - hy

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd