Explain counter stain - ziehl-neelsen method, Biology

Assignment Help:

Explain Counter Stain - Ziehl-Neelsen Method?

Methylene blue is used to stain previously decolourized cells. All non acid-fast bacteria de-stained by acid alcohol now absorbs counter stain and takes on its blue colour. All acid-fast bacteria remained red in colour due to primary stain.

Genus Mycobacterium shows acid fastness. These are aerobic, slightly curved or straight rods that sometimes branch or form filaments. These filaments readily break down into rods and coccoid bodies, when disturbed. Some grow very slowly. Some are free-living saprophytes and some are pathogens, e.g. M. bovis could cause tuberculosis in cattles, M. tuberculosis causes tuberculosis in humans, M leprae causes leprosy in humans. Acid-fast stain has a diagnostic value in identifying these pathogenic mycobacteria.


Related Discussions:- Explain counter stain - ziehl-neelsen method

Intervention strategy, Intervention Strategy There are five basic ways...

Intervention Strategy There are five basic ways in which government can correct market failures and ensure equity: inform, regulate, mandate, finance and deliver health care s

Explain about sex chromosomes, What is the other name given to sex chromos...

What is the other name given to sex chromosomes? Sex chromosomes are also known as allosomes (the other chromosomes that are not sex chromosomes are known as autosomes).

Gastrulation, GAS T RUL A TIO N - Morphogenic movements are occure...

GAS T RUL A TIO N - Morphogenic movements are occured. 3 germinal layers are formed. Ectoderm, endoderm & mesoderm. Endoderm is formed first. Mesoderm is formed in the

Locomotory structures, Locomotory Structures Annelids possess three k...

Locomotory Structures Annelids possess three kinds of locomotory structures, namely parapodia, setae and suckers. Parapodia are segmentally arranged, lateral, hollow extensio

Kidneys participate in the acid-basic equilibrium, Q. How do kidneys partic...

Q. How do kidneys participate in the regulation of the acid-basic equilibrium of the body? How are alkalosis and acidosis respectively corrected by the kidneys? Kidneys can reg

Explain alterations occurring in nuts and oilseeds, Alterations occurring i...

Alterations occurring in nuts and oilseeds Some oilseeds have acquired a great significance in the large-scale industrial production of edible oils.  You have already studied t

Explain the classification of carbohydrates, Define Classification of Carbo...

Define Classification of Carbohydrates? Carbohydrates are defined as polyhydroxy aldehydes or ketones or substances that produce such compounds when hydrolyzed. Carbohydrates a

What is joints in human biology, What is Joints Joints are locations...

What is Joints Joints are locations where two or more bones come together, or articulate. Bones are joined with varying degrees of rigidity. Joints may be fixed, as in the s

Define chlorophyll - natural colourant, Chlorophyll Chlorophyll is the...

Chlorophyll Chlorophyll is the porphyrin most widely used as a natural colourant. Its widespread occurrence in photosynthetic  tissue and its breakdown character have prompted

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd