Explain cosmid, Biology

Assignment Help:

Cosmid: The type of artificially constructed vector taken in use for cloning the 35-45 kb of DNA. These are plasmids carrying the phage l cos site (which permits packaging into l capsids), an origin of the replication and an antibiotic resistance gene. The plasmid of 40 kb is quite difficult to put into bacteria, but it can replicate once there. The cosmids, however, have the cos site, and hence can be packaged into l phage heads (the reaction which can be performed in vitro) to permit efficient introduction into the bacteria.


Related Discussions:- Explain cosmid

Types of surgery-pulmonary regurgitation, Types of Surgery :  Pulmonary va...

Types of Surgery :  Pulmonary valve replacement using pulmonary or aortic allograft is the procedure of choice. It increases R.V. ejection fraction, decreases R.V. end diastolic p

Function of amino acids, FUNCTIO N OF AMINO ACIDS Proteins are poly...

FUNCTIO N OF AMINO ACIDS Proteins are polymers of amino acids. Glycine form porphyrin nucleus in chlorophyll and heme (= haem) proteins like haemoglobin and cytochrome

Is herbivorism a form of predatism, Q. Is herbivorism a form of predatism? ...

Q. Is herbivorism a form of predatism? The Herbivorism is a form of predatism in which first order consumers feed from producers (plants or algae). For instance, fruits and bir

Properties of foods, Q. Properties of foods? various properties of food...

Q. Properties of foods? various properties of foods, under which the quality attributes of foods were introduced to you in terms of food quality and its categories as appearanc

Translation in eukaryotes, Transcription in eukaryotes, a much more complex...

Transcription in eukaryotes, a much more complex procedure than in prokaryotes. In the eukaryotes, translation and transcription take place in several cellular compartments that ar

What is the function of the testicles, Concerning reproduction what is the ...

Concerning reproduction what is the function of the testicles? The testicles are the male gonads, i.e., the organs where the production of gametes takes place. In human beings

Ventricular septal defect (vsd), Ventricular Septal Defect (VSD)   In v...

Ventricular Septal Defect (VSD)   In ventricular septal defect there is abnormal communication between right and left ventricle. Anatomically 90 per cent of all VSD are located

What is the numeric relation between purine bases, Q. What is the numeric r...

Q. What is the numeric relation between purine and pyrimidine bases in the DNA molecule? Is that relation valid in the RNA molecules? The DNA molecule is made of two bound poly

Phylum, description of first five phylum

description of first five phylum

Explain nutrient and storage proteins, Nutrient and Storage proteins Th...

Nutrient and Storage proteins These proteins store amino acids. Ovalbumin is the protein of egg white, used as an amino acid source for the developing embryo. Casein, the prote

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd