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Explain Basic Stains (Cationic) - Types of Stains?
These are chloride or sulfate salts of coloured bases which on ionization give positively charged chromogen. As such, these have a strong affinity for the negative constituents of the cell (or these bind strongly to negatively charged component of the cell), e.g. nucleic acid. Surface of the bacterial cells is also negatively charged, so basic dyes are most often used in bacteriology. These work better at high pH (i.e. neutral / alkaline conditions). For this reason, basic dyes are made up in alkaline solution, e.g. Loeffler's methylene blue stain is prepared in KOH solution.The pH can affect the staining effectiveness by affecting the nature and degree of charge on cell components. Whereas, in neutral or alkaline environment, microbial cells are negatively charged, in acidic environment, these stain poorly with basic dyes. Different staining techniques can be used to visualize and study the different morphological characteristics of a cell.
Clonal Propagation Most cultivars of ornamental and fruit species and forest trees are highly heterozygous. Consequently, their seed progeny is not true-to-type. To preserve
Explain the Ethological Approach in Taxonomy The use of behavioural or ethoIogica1 characteristics in animals is relatively new, though extremely useful, particularly in the id
A device called a hemocytometer is often used to estimate the number of cells in a culture. Cells are suspended in fluid. A small volume of the fluid is placed into a special chamb
Three molecules of ducose-6-phosphate yield 3 molecules of CO, and 3 molecules of five carbon residues (pentose sugar). The latter are converted ultimately to 2 molecules of g
Q. What are the major significant organic molecules for living beings? Ans. There are many types of organic molecules that are important for the living beings. Particularl
If you have water, 10% sucrose, and 40% sucrose solution. Which solutions were isotonic to each other?
The first movements of the foetus and appearance of hair on its head are usually observed during which month of pregnancy? 1. Fourth month 2. Fifth month 3. Sixth month
Classification of Protozoa Traditionally, protozoans have been classified as flagellates, amoebae, sporozoans and ciliates. We have retained this broad grouping for convenienc
Treatment Most cases of diarrhoea do not need antibiotic therapy as the bacterial or parasitic organism are not isolated from most of the cases. However chemotherapeutics ar
Characteristics of Gametes Following are some general characteristics of gametes: 1. The female gametes are usually laden with food material, the yolk, while the male gamet
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