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Explain Basic Stains (Cationic) - Types of Stains?
These are chloride or sulfate salts of coloured bases which on ionization give positively charged chromogen. As such, these have a strong affinity for the negative constituents of the cell (or these bind strongly to negatively charged component of the cell), e.g. nucleic acid. Surface of the bacterial cells is also negatively charged, so basic dyes are most often used in bacteriology. These work better at high pH (i.e. neutral / alkaline conditions). For this reason, basic dyes are made up in alkaline solution, e.g. Loeffler's methylene blue stain is prepared in KOH solution.The pH can affect the staining effectiveness by affecting the nature and degree of charge on cell components. Whereas, in neutral or alkaline environment, microbial cells are negatively charged, in acidic environment, these stain poorly with basic dyes. Different staining techniques can be used to visualize and study the different morphological characteristics of a cell.
You are given a hamster that has a very short tail (dominant trait) a) Describe a cross that would allow you to determine the short tailed hamster's genotype b) What are the possib
SEXUA L REPRODUCTION 1 . Amphigony - Zygote is formed. (i) Syngamy Fusion of gametes. (a) Endogamy - It involves self fertilisation. e.g.
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Q. What is signifying when it is said that beings of the phylum Annelida are vascular beings? From which other phyla of the animal kingdom does this feature differentiate them?
What are the scopes of Zoology?..
Apiculture : This is the study of bee-keeping for obtaining wax and honey. Beekeeping is also known as apiculture, it is taken from Latin apis, bee. Apiculture is the maintenance
What is L forms of bacteria?
A decrease in parasympathetic discharge to the heart leads to A. an increase in the conductance of F-channels in SA node cells. B. a decrease in the conductance of potassium
REPRODUCTIV E ISOLATION - According to the biological species concept, members of one species are reproductively isolated from members of all other species. Accordingly, re
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