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Explain Basic Stains (Cationic) - Types of Stains?
These are chloride or sulfate salts of coloured bases which on ionization give positively charged chromogen. As such, these have a strong affinity for the negative constituents of the cell (or these bind strongly to negatively charged component of the cell), e.g. nucleic acid. Surface of the bacterial cells is also negatively charged, so basic dyes are most often used in bacteriology. These work better at high pH (i.e. neutral / alkaline conditions). For this reason, basic dyes are made up in alkaline solution, e.g. Loeffler's methylene blue stain is prepared in KOH solution.The pH can affect the staining effectiveness by affecting the nature and degree of charge on cell components. Whereas, in neutral or alkaline environment, microbial cells are negatively charged, in acidic environment, these stain poorly with basic dyes. Different staining techniques can be used to visualize and study the different morphological characteristics of a cell.
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Explain Glycerol esters of fatty acids Glycerol esters of fatty acids, which make up to 99% of the lipids of plant and animal origin have been traditionally called fats and oi
Which is the type of nitrogen waste birds produce? Why does this feature, besides being an adaptation to the terrestrial environment, also mean an adaptation to flight? Birds a
Q. Explain about Rheology of Foods? Rheology is the study of stress and strain or in other words, it is the study of flow and deformation of materials, both liquids and solids,
In a properly functioning negative feedback system, the A. value of the controlled variable will always be very close to the threshold value when the system is in steady state.
Medical Management In chronic renal failure there is irreversible renal failure. The goals of medical management are: To promote maximal renal function To ma
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Explain about the Observation and Classification of Arthropoda? Introduction In the previous unit you have examined the representatives of artliropoda. In this unit, you wi
Selenium and vitamin E deficiency Selenium is an essential micro-mineral required for various physiological functions and for immunity against a variety of diseases. It is an i
Formation of Gemmules Throughout the formation of gemmules, masses of food - laden amoebocytes called archaeocytes feed on other cells and lay down in within them. They get s
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