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Explain Advantages and Disadvantages of Spread Plate Method?
The advantages and disadvantages of spread plate method are highlighted next.
Advantages
1. It is useful for the samples having heat sensitive microbes.
2. No subsurface colonies appear in spread plate so isolation of the organism is easy.
Disadvantages
Volume no greater than 0.1 ml can be spread on the nutrient agar plate because it would not soaken well and may result colonies to coalesce as they form.
Define Reagents for Preparation of Phosphate Buffers? Buffer solutions of pH4, pH7 and pH 9.18 phosphate buffer solution: 0.1 M disodium hydrogen phosphate or Na 2 HPO 4
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