Electron microscopy, Biology

Assignment Help:

ELECTRON MICROSCOPY (EM)

In EM, the object is viewed by a beam of electrons instead of visible light. An electron beam has a shorter wadelength (h = 0.004 nm) compared with visible light (h = 400 nm). Therefore, an EM has higher resalving power than LM. Till now electron microscopy is the best available method to study biological ultrastructure.

The resolving power of most modem EMS ranges between 100 pm to 0.1 nm. However, problems of specimen preparation, and radiation damage limit the resolution of biological objects to about 2 nm, even this is 100 times better than the resolution achieved by the best LMs. Further, the magnifying power of the EM is upto 200,000 X. The two types of EM are transmission electron microscope (TEM) and scanning electron microscope (SEM). about which you will study in the following section.


Related Discussions:- Electron microscopy

The klove roughness discrimination test, The Klove roughness Discrimination...

The Klove roughness Discrimination Test The subject must order four blocks covered with varying grades of sandpaper presented behind a blind with regards to degree of roughness

Three main types of trophic pyramids studied in ecology, What are the three...

What are the three main types of trophic pyramids studied in Ecology? The three kinds of trophic pyramids studied in Ecology are the numeric pyramid, the biomass pyramid and th

Penetrating injury, Penetrating Injury Open pneumothorax (sucking c...

Penetrating Injury Open pneumothorax (sucking chest wound)  Hemothorax  Tracheobrochiaf injury  Pulmonary contusion  Mediastinal injury

Determine the use of opg, Determine the use of  OPG An OPG is used to...

Determine the use of  OPG An OPG is used to see the possibility for an anterior loop which is found to extend about 1-3 mm anterior to the mental foramen in about 12 per cent

Genomic dna fragments, You have an extensive library of genomic DNA fragmen...

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate

Define heart rate (hr) method - aerobic capacity, Define Heart Rate (HR) M...

Define Heart Rate (HR) Method -  aerobic capacity? An alternative method for determining aerobic capacity involves the measurement of heart rate. Heart rate is linearly associa

How substrate concentration affect the enzymatic reaction, How does the sub...

How does the substrate concentration affect the speed of enzymatic reactions? Initially as substrate concentration increases, the speed of the reaction enhances; this happens b

What do you understand by haemagglutinins, Q. What do you understand by Hae...

Q. What do you understand by Haemagglutinins? Haemagglutinins are the globulin type of proteins which are present in the seeds of plants like double bean, field bean, white be

Simple or unilayered epithelia, SIMPL E EPITHELIA (UNILAYERED EPITHELIA) -...

SIMPL E EPITHELIA (UNILAYERED EPITHELIA) - 1 .      SIMPLE SQUAMOUS EPITHELIUM (PAVEMENT EPITHELIUM) - It is composed of large flat cells whose edges fit closely toge

Which areas of the globe is gymnosperm abundance noteworthy, Q. In which ar...

Q. In which areas of the globe is gymnosperm abundance noteworthy? These plants are the typical vegetation of cold regions like the boreal forest or taiga of the northern hemis

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd