Dot blot, Biology

Assignment Help:

Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitrocellulose or activated nylon, and many more), fixed and hybridized with the radioactive search. The extent of labeling (as determined by autoradiography and the densitometry) is proportional to concentration of the target molecule in its sample. Standards give the means of calibrating the results. 


Related Discussions:- Dot blot

Proteins of plant origin - seed proteins, Proteins of Plant Origin - Seed P...

Proteins of Plant Origin - Seed Proteins Seed Proteins: Although a large number of plants produce seeds having protein contents in excess of 15%, only a few are utilized for fo

Two biotic factors could affect an antelope, What two biotic factors could ...

What two biotic factors could affect an antelope living in the Serengeti? The biotic factors that may affect an antelope living in the Serengeti could be:predation by carnivore

What is paediatric clinical literature, What is paediatric clinical literat...

What is paediatric clinical literature The paediatric clinical literature has expanded greatly in its coverage of the wide variety of medical circumstances that can negatively

Explain congenital heart disease (chd), Explain Congenital Heart Disease (C...

Explain Congenital Heart Disease (CHD)? Congenital heart disease is an abnormality at birth in cardiac circulatory structure or function. Optimal management of CHD aims not mer

Aplastic anaemia, Aplastic Anaemia   Aplastic anaemia is a condition in...

Aplastic Anaemia   Aplastic anaemia is a condition in which production of red cells in the bone marrow is reduced. It involves failure to produce adequate red cells, white cell

Duodenum participating in extracellular digestion, Q. Besides the liver whi...

Q. Besides the liver which is the other adnexal gland of the digestive system that releases substances in the duodenum participating in extracellular digestion? The other adnex

Organization levels of life, Q. Define the Organization Levels of Life? ...

Q. Define the Organization Levels of Life? Ans. The cell is the basic unit of life - the smallest unit that can carry on all the processes of life. Organisms that consist of a

Vertebrate, Basic characters of vertebrate classes

Basic characters of vertebrate classes

Define neurological disorders of non- nutritional etiology, Define Neurolog...

Define Neurological disorders of non- nutritional etiology? Some of the common disorders are Alzheimer's disease, Parkinson's disease, epilepsy, and spinal and neuro trauma.

Explain precautions for isolation of pure culture, Explain Precautions for ...

Explain Precautions for Isolation of Pure Culture? 1. Plating of the medium should be done 24 hours before performing the streaking. There should not be any moisture on the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd