Dot blot, Biology

Assignment Help:

Dot blot is the technique for measuring the amount of one perticular DNA or RNA in the highly complex mixture. The samples are spotted onto the hybridization membrane (like nitrocellulose or activated nylon, and many more), fixed and hybridized with the radioactive search. The extent of labeling (as determined by autoradiography and the densitometry) is proportional to concentration of the target molecule in its sample. Standards give the means of calibrating the results. 


Related Discussions:- Dot blot

Use of alternate metabolic pathways, Use of Alternate Metabolic Pathways ...

Use of Alternate Metabolic Pathways Plants surviving under water-logged conditions offer the simplest example of such a response. The submerged parts of such plants use the an

In which strcture sperm cells go after passing epididymides, Q. After passi...

Q. After passing the epididymides through which structures do sperm cells go until exteriorization? After leaving the epididymis in the testicle sperm cells enters the vas defe

Planning of the nursing care, Planning of the Nursing Care 1) Educa...

Planning of the Nursing Care 1) Educate and teach the parents 2) Provide preoperative and postoperative care. Educate an Teach the Parents The parents may be

Cloning, there are a restriction map of a plasmid and a human gene of known...

there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI

Region of the injury in the axial skeleton, what major nerve do you think i...

what major nerve do you think is being compressed when a person often feel pain in the posterior surface of the thigh radiating to the area behind the knee and where is the likely

Define plate counts and colony forming unit, Define Plate Counts and Colony...

Define Plate Counts and Colony Forming Unit? Plate counts is the most frequently used method to measure microbial numbers. The technique, however, can be used only for those mi

Glands associated with eyes, GLANDS - 1 .       LACRYMAL GLANDS - ...

GLANDS - 1 .       LACRYMAL GLANDS - Tear glands numbers are 3. Its secretion is aquous & salty, keeps eye ball wet, give nourishment to cornea. Lysozyme also present

Biochemistry, Water is most dense and thus heavier at 4 degreese celceus. A...

Water is most dense and thus heavier at 4 degreese celceus. At 0 degreese celceus ice forms and can float on liquid water. Assuming that ice were most dense at 0 degreese celceus,

Explain the cyclosporin, Q. What is the cyclosporin? How are fungi related ...

Q. What is the cyclosporin? How are fungi related to this substance? Cyclosporin is a drug discovered in the 1970's that revolutionized organ transplantation in Medicine, It is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd