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Determination of Erythrocyte Transketolase (ETK) Activity?
The enzyme transketolase requires TPP for its activity, for the metabolism of pentose phosphate sugars as described under the functions earlier. ETK activity is expressed as basal (without TPP) activity (ETKA) or as the difference between stimulated and basal activity us n percentage of basal activity.
In thiamin deficiency, the enzyme activity is reduced (decreased ETKA) and a small part of the added pentose phosphate disappears. When TPP is added, ETK activity is increased. The percent increase in ETK activity due to added TPP, as indicated in the Table, is an index of the degree of thiamin deficiency.
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