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Which of the following is a true statement regarding the Eyes Absent Protein?
A. EYA is a transcriptional activator that having both a DNA binding domain and a transactivation domain thus it can bind to an enhancer and directly stimulate either RNA polymerase or the general transcription factor machinery.
B. EYA can activate transcription by interactions with RNA polymerase and the general transcription factor machinery but it does not bind directly to DNA therefore it must be recruited to enhancer elements by interactions with DNA binding proteins.
C. EYA is a DNA binding protein but lacks a transactivation domain thus it can bind to an enhancer but can only stimulate transcription by interactions with transcriptional co-activator proteins.
D. EYA lacks both DNA binding and transactivation domains thus it can only stimulate transcription by removing phosphate groups from RNA polymerase and the general transcription factor machinery
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Which technique would you use to A) see if a particular protein bound to a piece of DNA B) Detect exactly where on a piece of DNA a protein bound.
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