Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Disc Electrophoresis?
Disc electrophoresis has improved resolution and strong bands are obtained. It requires a two-gel system and several different buffers. The running gel in which the separation takes place is prepared and then overlaid by a short (1 cm), large- pored "Stacking" or "Spacer gel". The buffer in the lower reservoir and in the running gel is as described before, while that in the sample solution and in the stacking gel has a pH about two units less than that of the lower reservoir. When the current is switched on, the buffer ions from the upper reservoir migrate into the stacking gel as the stacking gel buffer ions migrate ahead of them. As this occurs, the upper reservoir buffer ions encounter a pH that is much lower than their pK.
They therefore assume their uncharged (or, in the case of glycine, zwitterionic) form and become electrophoretically immobile. This effect causes the macro-molecular ions to approach the running gel as stacks of very narrow (0.01 nm thick) bands or discs that are ordered according to their mobilities and lie between the migrating ions of the upper reservoir and those of the stacking gel. As the macromolecular ions enter the running gel, they slow down as a result of gel filtration effects. This permits the upper reservoir buffer ions to overtake the macromolecular bands and because of the running gel's higher pH, assume their fully charged form as they too enter the gel and electroporetic separation is achieved.
The compactness of the macromolecular bands entering the running gel greatly increases the resolution of the macromolecular separations. With a study of disc electrophoresis we come to an end of our study about electrophoresis. We hope you did not find the discussion presented in this section too technical. Just for your reference, we shall not be conducting any electrophoresis experiment during our laboratory work for this course, but it is important that you, as a student of dietetics, understand the concept and principle of electrophoresis clearly, since this is one important investigative technique used for research in the field of nutritional biochemistry. We suggest you now answer the questions included in the review question section included herewith. This will help you consolidate your understanding about electrophoresis.
What is HACCP? HACCP, as you may already know, is an acronym that stands for Hazard Analysis Critical Control Point, a systematic, science-based approach used in food
Define Supplementation to prevention of iron deficiency anaemia? Supplementation with low doses of iron is necessary to prevent anaemia in particular groups of people. Fortifie
Supenisory Nursing Round: Supervisory rounds are tours or excursions into areas where patients are cared for where personnel operate, where intra and inter departmental commun
Q. What is the phenomenon called as "red tide"? Which ambiental harms can it cause? Red tide is a phenomenon that takes place when dinoflagellates algae from the pyrrophyte gro
A proton accelerates from rest in a uniform electric field of 650 N/C. At some later time, its speed is 1.3 106 m/s. (a) Find the magnitude of the acceleration of the proton. m/s2
what is the shape of blood platelets
Amelioration of mineral deficiency Performance of livestock in the tropics is mainly governed by the quality and quantity of nutrients provided in the diet. In most of the dev
Tina administered 1 liter of sterile distilled water IV to a patient. Predict the direction (increase, decrease, no change) you would expect Tina's infusion to have produced in the
Aeration A well-aerated soil is one in which gases are available to plant roots and other soil organisms, in sufficient quantities and in proper proportions to support their no
The axons of all the baroreceptors in the body were destroyed at 2 AM. All else is normal. Which of the following statements is true for the system at 2:10 AM when compared to the
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd