Define role of buffer in electrophoresis, Biology

Assignment Help:

Define Role of Buffer in Electrophoresis?

Buffer ions have a two-fold purpose in electrophoresis, they carry the applied current, and they fix the pH at which electrophoresis is carried out and, thus, determine the kind of electrical charge on the solute and the extent of ionization of the solute. By these effects, the buffer determines the direction of the electrophoretic migration to one of the two electrodes.

The buffer ionic strength determines the thickness of the ionic cloud surrounding the charged molecules and, thus, also the rate of migration and the sharpness of the zones. Many buffer systems are used in electrophoretic procedures, but the most widely used are the barbital buffers and the Tris-boric acid EDTA buffers. Next, let us get to know about the different techniques of electrophoresis.


Related Discussions:- Define role of buffer in electrophoresis

What is mdrtb, What is MDRTB MDRTB - For patients with known exposure t...

What is MDRTB MDRTB - For patients with known exposure to multi-drug resistant TB (MDRTB) and a high risk of developing active TB, there are no data-based recommendations. Regi

Phylum annelida, what so special for phylum annelida

what so special for phylum annelida

Purposes of patient /client records, PURPOSES OF PATIENT's /CLIENT's RECO...

PURPOSES OF PATIENT's /CLIENT's RECORDS Following purposes are served by maintaining patient's/client's records: A means of communication.  A basis on which therap

Discovery of plant hormones, Discovery of Plant Hormones To date, five...

Discovery of Plant Hormones To date, five major classes of plant hormones have been discovered namely auxins, gibberellins, cytokinins, abscisic acid and ethylene. It is possi

How to do glucose test, How to do Glucose test A glucose test is a type...

How to do Glucose test A glucose test is a type of blood test used to determine the amount of glucose in the blood. There are several different kinds of glucose tests:  "

Show the artificial keys, Q. Show the Artificial Keys? These are based ...

Q. Show the Artificial Keys? These are based on the resemblances and differences of some prominent characters in the taxa. These are based on the phylogenetic relationship b

Etiologic factor of congestive cardiac failure, Q. Etiologic factor of Cong...

Q. Etiologic factor of Congestive Cardiac Failure? Congestive heart failure develops over a period of time when the necrotic tissues are not replaced by functional connective c

Latitudinal variations, Latitudinal Variations The latitudinal variatio...

Latitudinal Variations The latitudinal variation of temperature over the earth is the result of two main variables incoming solar radiation and the distribution of lan

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd