Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

Explain about the restriction enzymes, Which of the following is a false st...

Which of the following is a false statement regarding restriction enzymes? A. Restriction enzymes bind to a sequence specific element and disrupt the hydrogen bonds among

Iron deficiency anaemia, Iron Deficiency Anaemia   This is the most com...

Iron Deficiency Anaemia   This is the most common nutritional and haematologic disorder  in infancy and chhildhood in developing countries. It is caused by lack of sufficient i

Role of citric acid cycle, The citric acid cycle, also called as the tricar...

The citric acid cycle, also called as the tricarboxylic acid (TCA) cycle or Krebs cycle (after its discoverer in the year of 1937), is used to oxidize the pyruvate making during th

Define cercaria larva, Define Cercaria Larva? Cercaria  larva is the f...

Define Cercaria Larva? Cercaria  larva is the fourth  larval stage  in  the life-cycle of F, hepatica. It is a free living stage produced  by  the  redia  larva. 1. It has

Would you expect the muscle fibers of the tongue, Would you expect the musc...

Would you expect the muscle fibers of the tongue to be striated or smooth? What about the muscle of the diaphragm/ Explain your answer.

Why is cartilage more flexible than bone, Why is the cartilage more flexibl...

Why is the cartilage more flexible than bone? in general, why does cartilage take longer to repair than bone?

Mention chemical agents available for sterilization, Q. Mention chemical ag...

Q. Mention chemical agents available for sterilization? Various chemical agents available for sterilization are: Alcohols: ethyl alcohol, isopropyl alcohol, trichlorobuta

Define historical example for dynamical network, Define Historical example ...

Define Historical example for Dynamical Network? John Tyson constructed a nonlinear differential equation model representing the majority of the network of biochemical pathways

Explain digestion system, DIGESTION Digestive enzymes break down food p...

DIGESTION Digestive enzymes break down food particles into smaller units. You will see  that the final breakdown products of protein digestion are single amino acids or small c

Placenta - human development, Placenta - Human Development We had said...

Placenta - Human Development We had said previous in the unit that in the second week of development a primitive uteroplacental circulation is established. Early in this stage

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd