Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

What are the cytoplasmic structures present in animal cells, Q. What are th...

Q. What are the main cytoplasmic structures present in animal cells? The main cytoplasmic structures of the cell are the centrioles, the cytoskeleton, mitochondria, peroxisomes

Role of nucleic acids in metabolism, ROL E OF NUCLEIC ACIDS - These...

ROL E OF NUCLEIC ACIDS - These are DNA / RNA. Unit is nucleotides. Present in viruses and all cells. DNA is concerned as genetic material. RNA as genetic material

Effect of metamorphic hormones on gene expression, Effect of Metamorphic Ho...

Effect of Metamorphic Hormones on Gene Expression Effect of metamorphic hormones on gene expression in moulting and metamorphosing insects, the first report of a particular ho

How to investigate aortic stenosis by echo cardiography, Q. How to investig...

Q. How to investigate aortic stenosis by Echo Cardiography? 2D Echo cardiogram shows the number of cusps. But presence of raphae and distorted anatomy due to calcification may

Types of cambium, Types of Cambium On the basis of the arrangement of ...

Types of Cambium On the basis of the arrangement of the Fusiform cells as seen in tangential section, cambium is divided into: A) Storied/Stratified Cambium : The groups o

Animal diversity, what are the theories of animal classification

what are the theories of animal classification

Palliative and corrective procedures for cyanotic congenital, Palliative Pr...

Palliative Procedures   i)  This consists of enlarging the existing for a men ovale by putting a baloon through the defect (baloon septostomy) so that interatrial mixing of blo

How is litmus paper, How is litmus paper and testing for acid, base, and ne...

How is litmus paper and testing for acid, base, and neutral, related to biology?

Explain the techniques of operation in post myocardial, Explain the Techniq...

Explain the Techniques of Operation used in post myocardial infarction ventricular septal defect? Normal 0 false false false EN-IN X-NONE X-NONE

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd