Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

Budding - types of asexual reproduction, Budding - Types of Asexual Reprodu...

Budding - Types of Asexual Reproduction In reproduction through budding, small groups of cells form buds in some part or parts of the parent animal. The bud grows, differentia

Genetic drift, It is generally believed that genetic drift occurs as a resu...

It is generally believed that genetic drift occurs as a result of sampling error. As we said earlier it occurs in small populations such as peripheral isolates. We may demonstrate

Explain about chthalamus, Explain about chthalamus The realised niche o...

Explain about chthalamus The realised niche of Chthalamus is reduced from its fundamental niche and it is limited to the upper tidal zone Lower  limits Out-competed

Gas transportation systems in animals, Q. Oxygen comes from the environment...

Q. Oxygen comes from the environment and carbon dioxide in the end returns to the environment. How do small animals solve the problem of taking away and bringing these molecules fr

Vitamins - digestion process, Vitamins differ from minerals in that vitamin...

Vitamins differ from minerals in that vitamins are compounds, and not elements. Vitamins do not help form bodily structures and thus are needed strictly in small quantities-sometim

How to investigate aortic stenosis by cardiaccatheterization, Q. How to inv...

Q. How to investigate aortic stenosis by Cardiac Catheterization? The indications are to confirm hemodynamics when the non-invasive tests are not conclusive or there is a disc

What are the causes of constipation, Q. What are the Causes of constipation...

Q. What are the Causes of constipation? The most common causes of constipation are poor elimination habits, a lack of fibre in the diet, insufficient fluid intake, lack of exer

Myocardial protection-open heart surgery, Myocardial Protection :  To a gr...

Myocardial Protection :  To a great extent, the result of cardiac surgery depends on how well the myocardium is protected during cardio pulmonary bypass. Temporary depression of m

Observations reveal about mechanisms of sex determination, XYY humans are f...

XYY humans are fertile males. XXX humans are fertile females. What do these observations reveal about the mechanisms of sex determination and dosage compensation?

Transcription factors have multiple domains, In most cases, the transcripti...

In most cases, the transcription factors in eukaryotes which bind to promoter   or enhancer sequences   are activator   proteins   which induce   transcription.   That proteins gen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd