Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

Explain the adsorption or binding ability, Explain the Adsorption or Bindin...

Explain the Adsorption or Binding Ability? Some fibre components have the ability to bind (adsorb) substances in the gastrointestinal tract. Wheat bran, guar gum, mannan and is

What is the resting membrane voltage of the neuron, What is the resting mem...

What is the resting membrane voltage of the neuron A complete motor neuron is removed from a frog and placed in normal physiological saline at 1 AM.  The neuron is healthy.  At

What is extraoral examination, Extraoral Examination It includes examin...

Extraoral Examination It includes examining the following basic structures which are related to oral cavity. TMJ: Rule out any tenderness, crepitus, clicking or snapping

Potassium ions maintain the resting potential of neuron, Q. How do the pota...

Q. How do the potassium and sodium ions maintain the resting potential of the neuron? The plasma membrane of the neuron when at rest maintains an electric potential difference

Define nitrogen estimation - kjeldahl''s method, Define Nitrogen Estimation...

Define Nitrogen Estimation - Kjeldahl's Method? Nitrogen in the protein is estimated by a method referred to as Kjeldahl's method. Although over a period of time many other met

Class of coclentrata, ????? # 100 ??????????? #Minimum ?????? ?????

????? # 100 ??????????? #Minimum ?????? ?????

What are the typical vegetation of temperate forests, What are the typical ...

What are the typical vegetation and the typical fauna of the temperate forests? In the temperate forest deciduous trees predominate. Various Mammals are found in great number,

What is panoramic x-ray, What is Panoramic X-Ray This is one of the mos...

What is Panoramic X-Ray This is one of the most vital radiological techniques in planning placement of dental implants. Not only will it give you the complete picture of the pe

Risk characterization, Risk Characterization Risk  Characterization ...

Risk Characterization Risk  Characterization :  Integration  of  hazard  identification,  hazard characterization and exposure assessment into an estimation of the adverse e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd