Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

Determine the number of gametes, An organism has 16 chromosomes in its soma...

An organism has 16 chromosomes in its somatic cells. Determine the number of gametes with different chromosome compositions that it can potentially create. Explain your answer. Ign

Define procedure for detection of chalk in flour, Define Procedure for Dete...

Define Procedure for Detection of Chalk in Flour Carry out the experiment following the procedure given herewith: 1. Take a teaspoon of flour in a test tube and shake it wit

Ecology, Which of the following are likely to help conserve soil and preven...

Which of the following are likely to help conserve soil and prevent erosion?

Explain the disadvantage of contact plate method, Explain the Disadvantage ...

Explain the Disadvantage of Contact Plate Method? These plates are suitable for sampling the surfaces having low contamination, like pre-cleaned and sanitized surfaces. This is

Write the factorson on which metaboilc changes depend, The metaboilc change...

The metaboilc changes would depend on the  following  factors: a)  Nature of infection b)  Severity of the infection c)  Duration of the infection d)  Presence or abse

Explain about oral irrigation devices, Oral irrigation Devices The use ...

Oral irrigation Devices The use of oral irrigation devices has been controversial due to concerns about incomplete plaque removal and potential separation of connective issue f

Taxonomy, how do i classify mythical creatures into a web?

how do i classify mythical creatures into a web?

Mollusca, 5 classification of mollusco

5 classification of mollusco

What information from the patients symptoms, A 16 year old boy named Jake w...

A 16 year old boy named Jake was admitted into the emergency room complaining of headache, fever, stiffness of abdominal muscles and difficulty swallowing. The doctor on staff bega

Define the word solute, Define the word solute A solution is a homogeno...

Define the word solute A solution is a homogenous mixture of two or more different substances. For instance salt in water form a solution. This means that the dissolved subs

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd