Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

What a diploid cell contains, A diploid cell contains: A.one half of a comp...

A diploid cell contains: A.one half of a complete set of chromosomes B.several complete sets of chromosomes C.the correct number of chromosomes D.two complete sets of chromosomes E

Explain what is light microscope - microscopy, Explain what is Light Micros...

Explain what is Light Microscope - Microscopy Modern light microscopes are compound microscopes. Here the magnified image formed by the objective lens is further enlarged by on

Describe the types of drugs, Describe the types of Drugs • Induction a...

Describe the types of Drugs • Induction agent (thiopentone, fentanyl, ketamine, midazolam) • Suxamethonium (1-2 mg/kg) is the muscle relaxant of choice. Rapid sequence ind

Effects of cardiac output and hormones, Excluding the effects of cardiac ou...

Excluding the effects of cardiac output and hormones, describe the other factors that may affect blood pressure and blood flow in a middle-aged man who is exercising in an aerobics

What are the chronic complications of diabetes, Q. What are the Chronic com...

Q. What are the Chronic complications of diabetes? • Atherosclerosis: Degeneration of walls of the arteries due to fatty plaques - deposition on arterial walls. Diabetics ar

Pheromones serving functions other than reproduction, Pheromones Serving Fu...

Pheromones Serving Functions Other than Reproduction Besides sexual responses as described above pheromones mediate several other types of behaviour. Alarm pheromones have bee

Can you explain about cardiomyopathies, Q. Can you explain about Cardiomyop...

Q. Can you explain about Cardiomyopathies? Cardiomyopathy is a primary disorder of heart muscle that may cause cardiac dysfunction and is not related to any obvious disease pr

Use pcr to sequence the particular gene, You wish to use PCR to sequence a ...

You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volu

Embryos prom synergids, Embryos Prom Synergids The synergids, which u...

Embryos Prom Synergids The synergids, which usually degenerate prior to or soon after double fertilization, are reported to give rise to embryos in Argentine mexicana, Tamari

Explain about adhesive tape of many varieties, Explain about Adhesive tape ...

Explain about Adhesive tape of many varieties Adhesive tape of many varieties with backing materials coated with an adhesive to keep the dressing in place. The adhesive bandage

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd