Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

What is the contraceptive mechanism of the iud, What is the contraceptive m...

What is the contraceptive mechanism of the IUD? The IUD (intrauterine device) is a piece of plastic coated with copper that is inserted within the uterus by a doctor. Copper is

What is the cost-benefit relationship, What is the cost-benefit relationshi...

What is the cost-benefit relationship regarding sewage treatment as a method to fight water pollution? To treat sewage is much cheaper for society. The non treated sewage pollu

Contractile cells, CONTRACTI L E CELLS In addition to above mentioned...

CONTRACTI L E CELLS In addition to above mentioned three types of muscular tissue, there are contractile cells. These are as follows. 1.      Myoepithelial Cells (= M

Explain adverse effects of enfuvirtide, Adverse effects of Enfuvirtide ...

Adverse effects of Enfuvirtide Almost all patients make local injection site reactions to enfuvirtide, usually having of mild or moderate pain, erythema, induration, nodules an

Explain about steatorrhoea, Q. Explain about Steatorrhoea? Steatorrhoea...

Q. Explain about Steatorrhoea? Steatorrhoea is a symptom of the disorders of fat metabolism and malabsorption syndrome and can be defined as n condition of foul-smelling bulky

Avian tuberculosis, Avian tuberculosis The infection in poultry usuall...

Avian tuberculosis The infection in poultry usually occurs from ingestion of contaminated food and water. The lesions develop in spleen, liver and intestines; less frequently

Procedure of hormone act, Procedure of Hormone Act All plant hormones ...

Procedure of Hormone Act All plant hormones show extraordinary varied complex effects in controlling plant growth and development, Extrapolation from how an animal hormone wor

Anatomy of coronary circulation, The epicardial coronary artery system cons...

The epicardial coronary artery system consists of the left and right coronary arteries, which normally arise from ostia located in the left and right sinuses of Valsalva, respectiv

What is acute aortic regurgitation, Q. What is Acute Aortic Regurgitation? ...

Q. What is Acute Aortic Regurgitation? Infective endocarditis, aortic dissection and trauma often produce severe AR. Acute increase in left ventricular end diastolic volume es

Difference between lamarckism and neo-lamarckism, DIFFERENC E BETWEEN LAMA...

DIFFERENC E BETWEEN LAMARCKISM AND NEO-LAMARCKISM - S.No Lamarckism Neo-Lamarckism 1. It refers of the original

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd