Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

Electro magnetic radiation, Electro magnetic radiation Light is one ...

Electro magnetic radiation Light is one form of energy. The light rays consist of tiny particles called photons. The energy carried by photon is called quantum. Light

Penetrating injury, Penetrating Injury Open pneumothorax (sucking c...

Penetrating Injury Open pneumothorax (sucking chest wound)  Hemothorax  Tracheobrochiaf injury  Pulmonary contusion  Mediastinal injury

Protoplasmic - level of body organization, Protoplasmic - Level of body org...

Protoplasmic - Level of body organization Organisms which are made of just one cell are the simplest and the most primitive creatures called unicellular organisms. Their level

Male gamete and the female gamete in a flowering plant, What is (a) the mal...

What is (a) the male gamete, and (b) the female gamete in a flowering plant?   (a) The male gamete in a flowering plant is the pollen grain (strictly, the gamete is the male

Determine the use of opg, Determine the use of  OPG An OPG is used to...

Determine the use of  OPG An OPG is used to see the possibility for an anterior loop which is found to extend about 1-3 mm anterior to the mental foramen in about 12 per cent

Phylum nematoda (nemathelminthes ), PHYLUM  NEMATODA (= NEMATHELMINTHES ) ...

PHYLUM  NEMATODA (= NEMATHELMINTHES ) Definition and Introduction                                                                             Bilateral  and protostomial

Show the list of symptoms, Q. Show the list of symptoms? The list of sy...

Q. Show the list of symptoms? The list of symptoms includes: • Bulky, pale, loose, greasy and foul smelling stools. • Anorexia, feeling of fullness, pain abdomen. The

Endocrine versus neural integration, Endocrine versus Neural Integration ...

Endocrine versus Neural Integration A question that surely comes across your mind is, "what is the need for two types of integrative mechanisms, the neural and the endocrine"

What is the likely function of the snf gene, In yeast, a new mutation calle...

In yeast, a new mutation called suc was recently discovered.  The suc mutant cannot use sucrose as a source of carbon, and will not grow if the culture medium contains only sucrose

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd