Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

Dna (deoxyribonucleic acid), DNA (deoxyribonucleic acid) is the molecule w...

DNA (deoxyribonucleic acid) is the molecule which are responsible for storing and transmitting the genetic information. DNA is a double- stranded molecule which is held together b

Explain the effect of deficiency of pyridoxine, Explain the effect of Defic...

Explain the effect of Deficiency of pyridoxine? A deficiency of vitamin B6 alone is uncommon because it usually occurs in association with a deficit in other B-complex vitamin

Define aerobic exercise or aerobic energy system, Define Aerobic Exercise o...

Define Aerobic Exercise or Aerobic Energy System? To produce necessary energy, the body uses an aerobic pathway and an anaerobic pathway. Exercise that relies heavily on oxygen

Ketosis, K e to s i s It is also known as acetonemia in bovines or...

K e to s i s It is also known as acetonemia in bovines or pregnancy toxaemia in sheep and is associated with ketonemia, ketonuria and low blood glucose. E t iol

How does phosphocreatine act in the muscle contraction, How does phosphocre...

How does phosphocreatine act in the muscle contraction and relaxation? Phosphocreatine is the main means of energy storage of the muscle cells. During relaxed periods ATP mo

Explain the food applications of gum arabic, Explain the Food Applications ...

Explain the Food Applications of Gum Arabic A major use for gum Arabic is in the confectionery industry where it has two important functions; to retard or to prevent crysta

Cerebrum, what term is used to indicate its grooves?

what term is used to indicate its grooves?

What is autophagic intracellular digestion?, Autophagic intracellular diges...

Autophagic intracellular digestion is the cellular internal digestion of waste and residual materials. In general it is completed by lysosomes. Autophagic intracellular digestio

Explain why statement is logical in terms of kin selection, Biology once sa...

Biology once said why this statement is logical in terms of kin selection and use the coefficient of relatedness he'd be willing to lay down his life to save two brothers or eight

Explain the history of darwins finches, Explain the history of Darwin's Fin...

Explain the history of Darwin's Finches? The most well known evidence upon which Darwin based his theory was the distribution pattern of the Galapagos finches. The Beagle's voy

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd