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Define Molisch's Test or alpha naphthol reaction?
The Molisch test is a common test for the existence of carbohydrates.
Principle
The reaction is due to the formation of furfural or furfural derivatives. Furfural and furfural derivatives are derived from the dehydration of sugars. The dehydration is caused by the action of concentrated sulphuric acid on sugars. Disaccharides, oligosaccharides and polysaccharides are hydrolyzed to yield their repeating monomers by the acid. The alpha-naphthol reacts along with the cyclic aldehydes to make purple coloured condensation products.
Basic Instruments /Equipment Used In biochemical laboratories and important Working tips The first practical in this manual will orient you to the various equipment, apparatuses
.respiration in animals
ELIZA stands for enzyme linked immune diffusion assays. It works on standard of antigen antibody reaction.
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