Define instrument for absorbed radiation - spectrophotometer, Biology

Assignment Help:

Define Instrument for Absorbed Radiation - Spectrophotometer?

In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation into a spectrum producing a radiation of defined wavelength and subsequently measuring the intensity of that radiation. In place of defined wavelength and subsequently measuring the intensity of that radiation. In place of coloured filters, a diffraction grating or glass prism or quartz prism is fitted, which disperses the white light into a continuous spectrum. Total wavelength range, at which measurements can be made includes the visible spectrum (400-750 nm) and extends on each side into the near ultraviolet (360-400 nm) and near infra-red (750-1000 nm).

It is therefore possible to estimate substances which are more or less colourless in the visible region but which absorb light and are therefore coloured, in the ultraviolet or infrared region. These instruments will be available in the laboratory for you to carry out your experiments. Do use them carefully. You should also be aware of the different components/ parts of these instruments. Though it is not very essential, but this information will help you understand the functioning of these instruments more closely. A brief description about the essential parts of photocolorimeter is also included herewith in this section.


Related Discussions:- Define instrument for absorbed radiation - spectrophotometer

What are the zymogens, Q. What are the zymogens? proenzymes, or Zymogen...

Q. What are the zymogens? proenzymes, or Zymogens, are enzymes secreted in inactive form. Under some conditions a zymogen shifts to the active form of the enzyme. Zymogen secre

What are three main problems with having only two kingdoms, What are three ...

What are three main problems with having only two kingdoms There are three main problems with having only two kingdoms. The first problem concerns the unicellular flagellates

Photophosphorylation, explain the role of cyclic photophosphorylation in p...

explain the role of cyclic photophosphorylation in photosynthesis

Explain etiology and clinical feature of parkinson''s disease, Explain the ...

Explain the Etiology and Clinical Features of Parkinson's disease? The cause of Parkinson's is unknown. Genetic predisposition (in most cases the reason for the death of these

What do you mean by borrowed materials, Q. What do you mean by Borrowed Mat...

Q. What do you mean by Borrowed Materials? For the study of as much relevant material as possible the material is obtained on loan. Borrowed specimens benefit the' lending inst

Importance of the brain evolved from fishes to reptiles, Q. How has the imp...

Q. How has the importance of the brain evolved from fishes to reptiles? From the least to the most complex brain structure, it is evident that the brain, from fishes to beings

Nutrition education for prevention of vitamin a deficiency, Define Nutritio...

Define Nutrition Education for prevention of vitamin a deficiency? Ignorance, you may recall studying earlier, is an important determinant of vitamin A deficiency. There is, th

Objectives of taxonomy, Q. Objectives of taxonomy? Plants and animals b...

Q. Objectives of taxonomy? Plants and animals both have a great significance and unparalleled impact on human life. Most of our needs are fulfilled with plants such as foodstuf

Seed dispersal, what are the characteristics of the stages in seed dispersa...

what are the characteristics of the stages in seed dispersal

Cellular respiration, Cellular respiration is the transfer of energy from ...

Cellular respiration is the transfer of energy from various molecules to produce ATP; takes place in the mitochondria of the eukaryotes, the cytoplasm of prokaryotes. In the proce

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd