Define instrument for absorbed radiation - spectrophotometer, Biology

Assignment Help:

Define Instrument for Absorbed Radiation - Spectrophotometer?

In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation into a spectrum producing a radiation of defined wavelength and subsequently measuring the intensity of that radiation. In place of defined wavelength and subsequently measuring the intensity of that radiation. In place of coloured filters, a diffraction grating or glass prism or quartz prism is fitted, which disperses the white light into a continuous spectrum. Total wavelength range, at which measurements can be made includes the visible spectrum (400-750 nm) and extends on each side into the near ultraviolet (360-400 nm) and near infra-red (750-1000 nm).

It is therefore possible to estimate substances which are more or less colourless in the visible region but which absorb light and are therefore coloured, in the ultraviolet or infrared region. These instruments will be available in the laboratory for you to carry out your experiments. Do use them carefully. You should also be aware of the different components/ parts of these instruments. Though it is not very essential, but this information will help you understand the functioning of these instruments more closely. A brief description about the essential parts of photocolorimeter is also included herewith in this section.


Related Discussions:- Define instrument for absorbed radiation - spectrophotometer

Treatment of congestive cardiac failure, Q. Treatment of congestive cardiac...

Q. Treatment of congestive cardiac failure? A judicious and careful co-ordination between oxygen support, drug therapy and nutrient intake can help in alleviating majority of t

Types of community, Types of Community On the basis of size and degree ...

Types of Community On the basis of size and degree of relative independence communities may be divided into two types: i) Major Community: These are large-sized, well org

Handling microorganisms, Handling : You can reduce direct contact with mic...

Handling : You can reduce direct contact with microorganisms by careful handling and by wearing protective clothing such as rubber gloves, mask, lab coat, etc. Even if you entirel

The human impact on the environment, put the following events in the most p...

put the following events in the most probable order. A. Dead algae decomposed by bacteria B.excess nitrate and phosphate discharge into rivers C. Fish die of suffocation D.bacteria

What is coronary bypass graft, Q. What is coronary bypass graft? The Co...

Q. What is coronary bypass graft? The Coronary artery bypass graft is a kind of surgical myocardial revascularization that is a way to provide blood to a myocardium whose blood

Discuss the genetics involved in the inheritance, Casein is the main protei...

Casein is the main protein in milk. There are three types of casein, one of which is beta casein. Beta casein comes in two main variants called A1 and A2. The order of amino acids

What is the critical photoperiod, What is the critical photoperiod? How can...

What is the critical photoperiod? How can the critical photoperiod relate to flowering be experimentally determined? The critical photoperiod is the limit of the photoperiod du

Explain fliylurn basidiomycetes, Fliylurn Basidiomycetes 1) The asexual...

Fliylurn Basidiomycetes 1) The asexual reproduction is by means of basidiospores which are produced on a microscopic, club-shaped basidium. 2) The basidia are found in a fru

Sources of food contamination, All plants and animals have a natural microf...

All plants and animals have a natural microflora associated with them. For example, the plants have a natural microflora associated with the surface of roots, stems, leaves e

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd