Define instrument for absorbed radiation - spectrophotometer, Biology

Assignment Help:

Define Instrument for Absorbed Radiation - Spectrophotometer?

In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation into a spectrum producing a radiation of defined wavelength and subsequently measuring the intensity of that radiation. In place of defined wavelength and subsequently measuring the intensity of that radiation. In place of coloured filters, a diffraction grating or glass prism or quartz prism is fitted, which disperses the white light into a continuous spectrum. Total wavelength range, at which measurements can be made includes the visible spectrum (400-750 nm) and extends on each side into the near ultraviolet (360-400 nm) and near infra-red (750-1000 nm).

It is therefore possible to estimate substances which are more or less colourless in the visible region but which absorb light and are therefore coloured, in the ultraviolet or infrared region. These instruments will be available in the laboratory for you to carry out your experiments. Do use them carefully. You should also be aware of the different components/ parts of these instruments. Though it is not very essential, but this information will help you understand the functioning of these instruments more closely. A brief description about the essential parts of photocolorimeter is also included herewith in this section.


Related Discussions:- Define instrument for absorbed radiation - spectrophotometer

Define the objectives in the biochemistry of the eye, Define the objectives...

Define the objectives in the biochemistry of the eye. After going by this section, you should be familiar with: a. Dynamic of vascular supply of eye; b. The cornea metabo

Optical rotation in organice compounds, how the optical rotaion occurs in g...

how the optical rotaion occurs in glucose and ribose?

Determine the uses of iron - soil, Determine the uses of Iron - Soil Ir...

Determine the uses of Iron - Soil Iron is a constituent of cytochromes, haem and non-haem enzymes. It is also essential for chlorophyll formation though is not a part of it. It

Assessment of aplastic anaemia, Assessment   The patient will present w...

Assessment   The patient will present with striking pallor, irritability and lethargy due to anaemia, bledding or bruising and petechiae due to thrombocytopenia, fever and infe

Monounsatuated fats required for dyslipidemia, Q. Monounsatuated fats requi...

Q. Monounsatuated fats required for dyslipidemia? Monounsatuated fats (MUFA): These are liquid at room temperature, the highest food source being olive oil, canola oil, rapesee

Example of positive feedback of the homeostatic regulation, Q. What is an e...

Q. What is an example of positive feedback of the homeostatic regulation? In positive feedback the effect caused by an action stimulates the action even more. This is a rarer m

Plant biodiversity, discuss why obelia is considered to be a special organi...

discuss why obelia is considered to be a special organism in zoology

Heat exchangers, Normal 0 false false false EN-IN X-N...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Calcium - mineral elements, CALCIUM It is the most abundant mineral of...

CALCIUM It is the most abundant mineral of animal body. Calcium is available in all types of vegetables, grain, milk, cheese, eggs, fish and butter. By its deficiency r

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd