Define instrument for absorbed radiation - spectrophotometer, Biology

Assignment Help:

Define Instrument for Absorbed Radiation - Spectrophotometer?

In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation into a spectrum producing a radiation of defined wavelength and subsequently measuring the intensity of that radiation. In place of defined wavelength and subsequently measuring the intensity of that radiation. In place of coloured filters, a diffraction grating or glass prism or quartz prism is fitted, which disperses the white light into a continuous spectrum. Total wavelength range, at which measurements can be made includes the visible spectrum (400-750 nm) and extends on each side into the near ultraviolet (360-400 nm) and near infra-red (750-1000 nm).

It is therefore possible to estimate substances which are more or less colourless in the visible region but which absorb light and are therefore coloured, in the ultraviolet or infrared region. These instruments will be available in the laboratory for you to carry out your experiments. Do use them carefully. You should also be aware of the different components/ parts of these instruments. Though it is not very essential, but this information will help you understand the functioning of these instruments more closely. A brief description about the essential parts of photocolorimeter is also included herewith in this section.


Related Discussions:- Define instrument for absorbed radiation - spectrophotometer

What is human glucose transporters (glut), What is Human Glucose Transporte...

What is Human Glucose Transporters (GLUT)? All GLUTS are the integral proteins, which penetrate and span the lipid bilayer of plasma membrane. Six isomers of GLUT have been des

Define precautions for acid fast staining, Define Precautions for Acid Fast...

Define Precautions for Acid Fast Staining? 1. Do not allow carbol fuchsin to dry on heating. 2. Maintain aseptic conditions. 3. Decolourization should be done till no red

Indirect routes of disease transmission, Question 1: Define Survey. Lis...

Question 1: Define Survey. List out advantages of surveys. Brief up different survey methods Definition of survey. Advantages of surveys Different survey methods

Circulatory system, Circulatio n I n  Animal Circulation is served ...

Circulatio n I n  Animal Circulation is served by a fluid as blood/ lymph that circulates through the body in a regular manner, receiving & distributing materials in the wa

Name the final stage of cell division, During final stage of cell division,...

During final stage of cell division, mitotic apparatus disappears, chromosomes become attenuated, centrioles duplicate and split, nuclear membrane becomes reconstituted and nucleol

Protozoans and cnidarians, compare and contrast the structure of the follow...

compare and contrast the structure of the following: hyrda plain, hydra mematocysts, obelia, amoeba, euglena, paramecium caudatum conjugation, euglena acus.

Name chemical elements that form living biological matter, What are the che...

What are the chemical elements that form most of living biological matter? The elements are:- a)  oxygen (O), b)  carbon (C), c)  hydrogen (H) and d) Nitrogen (N).

Basic research terms - nursing research, Basic Research Terms: Some of...

Basic Research Terms: Some of the basic  terms that are defined  in  this section are:  Assumptions Operational Definitions  Variables  Delimitation and Limitation  H

Energy metabolism, Energy Metabolism In the preceding sections of the ...

Energy Metabolism In the preceding sections of the unit, you studied how the products of digestion of food, viz: amino acids, sugars and fatty acids are absorbed and transport

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd