Define instrument for absorbed radiation - spectrophotometer, Biology

Assignment Help:

Define Instrument for Absorbed Radiation - Spectrophotometer?

In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation into a spectrum producing a radiation of defined wavelength and subsequently measuring the intensity of that radiation. In place of defined wavelength and subsequently measuring the intensity of that radiation. In place of coloured filters, a diffraction grating or glass prism or quartz prism is fitted, which disperses the white light into a continuous spectrum. Total wavelength range, at which measurements can be made includes the visible spectrum (400-750 nm) and extends on each side into the near ultraviolet (360-400 nm) and near infra-red (750-1000 nm).

It is therefore possible to estimate substances which are more or less colourless in the visible region but which absorb light and are therefore coloured, in the ultraviolet or infrared region. These instruments will be available in the laboratory for you to carry out your experiments. Do use them carefully. You should also be aware of the different components/ parts of these instruments. Though it is not very essential, but this information will help you understand the functioning of these instruments more closely. A brief description about the essential parts of photocolorimeter is also included herewith in this section.


Related Discussions:- Define instrument for absorbed radiation - spectrophotometer

Name the two hormones produced by the pancreas, Name the two hormones produ...

Name the two hormones produced by the pancreas and say (a) in what circumstances, (b) in what way, they adjust the glucose concentration in the blood.

Cell wall, CELL WALL Discovered by Robert Hooke , 1665 when he saw ...

CELL WALL Discovered by Robert Hooke , 1665 when he saw dead empty cork cells. Bonne r - studied the chemical nature of cell wall. The cell wall is a rigid & prote

How sugar is used in icings, How sugar is used in Icings? Sugars provid...

How sugar is used in Icings? Sugars provide sweetness, flavor, bulk and structure to icings and frostings. In addition to sweetness and flavor, icings also function as a barrie

Microbiology, Applications of microbiology in different fields

Applications of microbiology in different fields

Induction of disease resistance in plants by heat-shock, Induction of disea...

Induction of disease resistance in plants by heat-shock The fungus Cladosporium cucumerinum infects cucumber and causes disease in the plant. However, if seedling of cucumber

Protein synthesis with dna, Protein Synthesis with DNA The central dogm...

Protein Synthesis with DNA The central dogma of modern biochemistry is totally based on the coded information holded within deoxyribonucleic acid (DNA). Double stranded DNA is

Define metabolic response to food - requirement of energy, Define Metabolic...

Define Metabolic response to food - Requirement of Energy? Eating requires energy for the ingestion and digestion of food, and for the absorption, transport, inter conversion,

Periphyton - aquatic ecosystem, Periphyton - Aquatic Ecosystem These a...

Periphyton - Aquatic Ecosystem These are organisms which remain attached or clinging to stems and leaves of rooted plants or substances emerging above the bottom mud. Usually

Cultured plant cells, Cultured plant cells: Cultured plant cells are re...

Cultured plant cells: Cultured plant cells are recognized to give biochemicals of interest since  1950's , but starting the yields were very low. Refined culture structures hav

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd