Define instrument for absorbed radiation - spectrophotometer, Biology

Assignment Help:

Define Instrument for Absorbed Radiation - Spectrophotometer?

In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation into a spectrum producing a radiation of defined wavelength and subsequently measuring the intensity of that radiation. In place of defined wavelength and subsequently measuring the intensity of that radiation. In place of coloured filters, a diffraction grating or glass prism or quartz prism is fitted, which disperses the white light into a continuous spectrum. Total wavelength range, at which measurements can be made includes the visible spectrum (400-750 nm) and extends on each side into the near ultraviolet (360-400 nm) and near infra-red (750-1000 nm).

It is therefore possible to estimate substances which are more or less colourless in the visible region but which absorb light and are therefore coloured, in the ultraviolet or infrared region. These instruments will be available in the laboratory for you to carry out your experiments. Do use them carefully. You should also be aware of the different components/ parts of these instruments. Though it is not very essential, but this information will help you understand the functioning of these instruments more closely. A brief description about the essential parts of photocolorimeter is also included herewith in this section.


Related Discussions:- Define instrument for absorbed radiation - spectrophotometer

Explain interferon alfa, Interferon alfa In about one third of adults a...

Interferon alfa In about one third of adults and children with chronic hepatitis B, treatment with interferon alfa-2b leads to loss of HBeAg, return to normal aminotransferase

Transport of macromolecules, Nuclear transport is an energy-dependent proce...

Nuclear transport is an energy-dependent process mediated through saturable receptors. Export and Import receptors are by to distinguish and bind to nuclear localization signals or

Topical route for injection, Topical Route The medication in topical r...

Topical Route The medication in topical route is administered through ear, nose and eye. Drops are instilled into the nose, ear and eyes of the child in much the same way

Reprouction, which hormone is secret in salivary gland?

which hormone is secret in salivary gland?

Explain the objectives of history of mart disease, Explain the OBJECTIVES o...

Explain the OBJECTIVES of history of mart disease? After reading this unit, you should be able to: 1. Understand the importance of Cardio-vascular diseases as the leading cause

Pericardium, The heart is enclosed in a membranous sac called the pericardi...

The heart is enclosed in a membranous sac called the pericardium. It has two layers- the fibrous pericardium which is the outer layer and the serous pericardium that lies inside th

Male reproductive disorders-testicular hypoplasia, Testicular Hypoplasia ...

Testicular Hypoplasia Hypoplasia refers to testicular disorders related to congenitally underdeveloped and underactive spermatic epithelium of one or both testes even though t

Are alleles segregate during meosis, Gregor mendel was an austrain augustia...

Gregor mendel was an austrain augustianian monk; his work most notably showed that. a. Alleles segregate during meosis b. Capital alleles are dominant c. The genetic material is pa

Workplace exposure limits, Question 1: What are the heat-induced disord...

Question 1: What are the heat-induced disorders that may result from excessive exposure to a hot working environment? How can we alleviate such health problems? Question 2:

Explain the food applications of carrageenan, Food Applications of Carragee...

Food Applications of Carrageenan Carrageenan consists of a family of hydrocolloids, which have different properties and it has a wide variety of uses. Some examples of properti

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd