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Define Function of thiamin as Regulator of enzyme activity?
Thiamin regulates the enzymes involved in carbohydrate metabolism. These are:
a) Pyruvate dehydrogenase, which provides a key link between glycolytic pathway and citric acid cycle.
b) α-ketoglutarate dehydrogenase in citric acid cycle and transketolase involved in pentose phosphate pathway.
c) Each enzyme contains a decarboxylase moiety that binds TPB at the active site, a lipoic acid binding moiety, a flavoprotein and one or more regulatory components that toggle the enzyme complex between the active form and the inactive form.
d) A fourth thiamin requiring enzyme is a branched-chain ketoacid dehydrogerzase, which plays a role in the metabolism of branched-chain amino acids.
Determine sequence weights for the sequences ACTA, ACTT, CGTT, and AGAT by using Thompson, Higgins, and Gibson method
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