Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Characteristic feature of respiration, Characteristic Feature of Respiratio...

Characteristic Feature of Respiration From our opinion we may say that the characteristic feature of respiration is the intake of oxygen and the output of the carbon dioxide.

Carbohydrates requirement during diabetes, Q. Carbohydrates requirement dur...

Q. Carbohydrates requirement during diabetes? Carbohydrates: These to a large extent depend on the food habits. Complex carbohydrates with more fibre are recommended to simple

Ecology, Types of survivorship curves

Types of survivorship curves

Taxonmy, classification on taxonomy

classification on taxonomy

Signify the glands which are located at upper end of kidney, Part of the en...

Part of the endocrine system in humans, these 2 glands are small bodies located at upper end of every kidney. While these glands perform a range of functions, two of the most signi

What is intermittent st depression, What is Intermittent ST Depression ? ...

What is Intermittent ST Depression ? Ans. Several patients progress from variable ST-segment depression, often associated with respiration, to the classic ST-segment chang

Global Warming Prevention, Preventive steps for global warming :  Growing ...

Preventive steps for global warming :  Growing trees in vacant places. Growing social forests and protect them. Promoting abundant growth of phytoplankton plant species in marine

Explain class angiospermae, Class angiospermae (Flowering plants) Flowers...

Class angiospermae (Flowering plants) Flowers are the reproductive structures. Ovules are protected within ovary, xylem vessels are present. After fertilisation the ovary develop

Differance between descending thoracic or abdominal aneurysm, Differance be...

Differance between Descending Thoracic or Abdominal Aneurysm ? Descending Thoracic Aortic and Thoraco-Abdominal Aneurysm :  The approach is through n left postero-lat

Explain the maintenance requirement of nutritional needs, Explain the Maint...

Explain the Maintenance Requirement of nutritional needs? This is defined as 'the amount of nutrient that is needed to replace the wear and tear of the tissues within the body

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd