Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
CARDIO PULMONARY BYPASS : Open-heart surgery is considered as one of the most significant advances in medicine of 20th century. Establishment of safe cardio pulmonary bypass (CP
Classification according to the modes of transmission 1. Direct zoonoses: The direct zoonoses are those zoonoses that are transmitted from an infected vertebrate host to a
What happens when the cell membrane or plasma membrane ruptures or breaks down? When cell membrane ruptures Ions leek out and if not repaired in time the cell will die. As we k
what are the function of nucleus
Define Instrument for measurement of absorbed radiation-Colorimeter? The simplest types of, photometric instruments are designed for measurements in the visible region of spect
How did the experiments of Redi and Pasteur refute the hypothesis of spontaneous generation? To refute the impulsive generation hypothesis many experiments were performed. The
Fliylurn Basidiomycetes 1) The asexual reproduction is by means of basidiospores which are produced on a microscopic, club-shaped basidium. 2) The basidia are found in a fru
Q. What is Sick Sinus Syndrome? Ans. When a slow resting pulse fails to accelerate normally with exercise, it has been labeled chronotropic incompetence, however, there i
Explain Taxonomists as Synthesisers? Your study of the earlier units of taxonomy, in particular its history must have made you aware that the field of taxonomy has changed cons
Clinical Evaluation in maintenance in the Continuing Care Cycle Examination of gingival contours, along with mechanical evaluation of the prosthesis, implants, and implant comp
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd