Define about the cellulose acetate electrophoresis (cae), Biology

Assignment Help:

Define about the Cellulose acetate electrophoresis (CAE)?

If the hydroxyl groups in cellulose are reacted with acetic anhydride, cellulose is acetylated to form the raw material for cellulose acetate membranes. The characteristics of the membrane will vary with the extent of acetylation, the pore size and thickness of the membranes etc. All forms have advantages over paper. The material is microporous with pore size from 1 to 5 µm for dry membranes, such pore sizes prevent spreading of the bands or spots so that plasma proteins are finely resolved even at low voltages. Because of the higher resolution, cellulose acetate is used to separate isoenzymes, lipoproteins, haemoglobins and for immuno electrophoresis.

Smaller samples can be examined while maintaining high resolution and the greater sensitivity makes the procedure suitable for screening purposes when many samples can be applied to a single strip and run for a short time, thus facilitating visual comparison. Minimal protein adsorption eliminates trailing and gives an almost white background after washing. Cellulose acetate strip holds little water, so loss by evaporation must be minimized by using buffers of lower ionic strength to reduce the current and the heating effect at a given potential gradient. The migration rate and band width vary inversely with buffer concentration. For best results, fresh buffer should be employed for each run by using just enough buffers to cover the bottom of the tank. Where bubbles are present between compartments, wicks which reach to the bottom of the tank may be used. An advantage of CAE is the speed of separation (20 minutes to 1 hour) and the ability to store the transparent membranes for long periods.


Related Discussions:- Define about the cellulose acetate electrophoresis (cae)

Improvement of cereal proteins - mutual supplementation, Define Improvement...

Define Improvement of cereal proteins - Mutual supplementation? Improvement of cereal proteins: Cereals, in general, are limiting in lysine and threonine while legumes, milk, m

Microbiology and parasitology, Describe the methods that are used for the i...

Describe the methods that are used for the isolation and identification of bacteria.(20 marks)

The Endocrine system, What are the two models that explain Mechanism of Hor...

What are the two models that explain Mechanism of Hormone Action on their target?

Physiology, what happens to your total peripheral resistance when you go fr...

what happens to your total peripheral resistance when you go from a warm room to cool outside air

Copulation, COPUL A TIO N - Followed with the help of penis. Sperms ...

COPUL A TIO N - Followed with the help of penis. Sperms are ejaculated deep in vagina. Polyestrous i.e. can capulate any time thorughout the year.

To observe yeast plants, To observe yeast plants Borrow a microscope fr...

To observe yeast plants Borrow a microscope from a college, a high school, a doctor or a hospital. Place a some drops of the sugar solution which having yeast on a glass slide

What are enantiomers, Enantiomers: Select one: a. Have the same molec...

Enantiomers: Select one: a. Have the same molecular weight b. Have the same connectivity c. Are mirror images d. Are non-superimposable e. All of the above

Functions of skeleton, FUNCTIONS OF SKELETON - 1.      Support. 2.  ...

FUNCTIONS OF SKELETON - 1.      Support. 2.      To give shape to the body. 3.      Protection of different organs. 4.       Site for muscle attachment. 5.       He

#zoology, #what is naturalistic theory?

#what is naturalistic theory?

Biological responses controlled by phytochrome, Biological Responses Contro...

Biological Responses Controlled by Phytochrome Phytochrome responses are those which are controlled reversibly by red and far-red light. These can be broadly categorised as:

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd