Chromosome walking, Biology

Assignment Help:

Chromosome walking is the technique for cloning everything in genome around the known piece of DNA (the begning probe). You screen the genomic library for all the clones hybridizing with probe, and then figure out which one extends furthest into surrounding DNA. The most distal segment of this most distal clone is then taken in use as a probe, so that ever more distal areas can be cloned. This has been taken in use to move as much as 200 kb away from the given begning point (an immense undertaking). In general it is used to "walk" from a starting point towards some nearby gene in order to clone that specific gene. It is also used to obtain the remainder of a gene when you have isolated a part of it.


Related Discussions:- Chromosome walking

Simple squamous epithelium - stratified squamous epithelium, Q. How differe...

Q. How different is the simple squamous epithelium from the stratified squamous epithelium? Where can these epithelia are found in the human body? The simple squamous epitheliu

Types of aortic stenosis-aortic stenosis-valve disease, Types of Aortic Ste...

Types of Aortic Stenosis:  Obstruction to left ventricular outflow is commonly at the valvar level. Less commonly it is at the sub valvar or supra valvar level. Sub valvar ob

Describe the genetic material of a virus, Q What is the genetic material of...

Q What is the genetic material of a virus? How does that material act in viral reproduction? There is DNA viruses double strand or single strand DNA and RNA viruses double stra

What are flight adaptations present by birds, Q. What are flight adaptation...

Q. What are flight adaptations present by birds? Wings associated to a well-developed pectoral musculature, less accumulation, pneumatic bones of feces in the bowels due to the

Define supplementary feeding to control under nutrition, Define Supplementa...

Define Supplementary feeding to control under nutrition? Supplementary feeding has remained an important component to control under nutrition. Considering the dietary inadequac

Explain the working of skeletal muscle, Explain the working of Skeletal Mus...

Explain the working of Skeletal Muscle? Skeletal muscle is under voluntary control and is responsible for voluntary movements of body parts. Skeletal muscle is also called stri

Chlorofluorocarbons cause global problem, In what way do chlorofluorocarbon...

In what way do chlorofluorocarbons (CFCs) cause global problems? Chlorofluorocarbons react with and deplete the ozone in the ozone layer. This permits more ultra-violet light f

Branchiostoma, What is the feeding habitat of branchiostoma?

What is the feeding habitat of branchiostoma?

Define the term probiotics, Normal 0 false false false ...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

What substance is prepared from species of red algae, Name that gelatin-lik...

Name that gelatin-like substance which is prepared from different species of red algae growing in Asiatic waters. Prepared product appears in the form of cakes, coarse granules and

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd