biochemistry, Science

Assignment Help:
Ask quTo optimise your kinase assay you require a range of substrate concentrations in the assay starting from 10 µM down to 10 nM substrate in 50 µL total assay volume (use 1:10 dilution steps). Calculate the concentrations required for the substrate stock solutions allowing addition of 10 µL to the kinase assay.
The substrate concentrations have to be how many times higher in the substrate stock solution than in the final kinase assay?
Select one:
a. 20x
b. 5x
c. 50x
d. 4x
e. 2x

Related Discussions:- biochemistry

Broad area, descibe the factors which decidethe broad area of scientific ac...

descibe the factors which decidethe broad area of scientific activity?

Explain the lubrication of food, Explain the Lubrication of food Dry fo...

Explain the Lubrication of food Dry food enters the mouth. It is moistened and lubricated by the saliva, before it can be made into a bolus ready for swallowing.

Explain ablation, What is ablation? Ans)  Ablation is a technological t...

What is ablation? Ans)  Ablation is a technological term for the loss of snow and ice from a glacier. The process happens by a combination of the two physical processes, meltin

Physico-chemical properties of vitamin e, Physico-chemical properties of vi...

Physico-chemical properties of vitamin E are as follows: a)  Tocopherols are yellow, oily liquids. b)  They are freely soluble in fat solvents. c)  The  compounds  are

Useful instruction for stock verification and maintenance , Useful Instruct...

Useful Instruction : You should go through of LT-1 course before doing this exercise. This exercise is a mix of demonstration and hands on activities. Take the help of your counse

How can percentage of solid particle been calculated, How can percentage of...

How can percentage of solid particle been calculated The percentage of solid particles and pore space in a soil may be calculated from the bulk density and particle density if

Explain the soil texture and methods of analysis, Soil Texture and Methods ...

Soil Texture and Methods of Analysis Soil texture is the basic property of a soil  related to the  size of individual mineral particles, which cannot be easily altered.  It spe

Nutrition, Obesity - Choose one macronutrient (carbohydrates) and one mic...

Obesity - Choose one macronutrient (carbohydrates) and one micronutrient (vitamins) that have some relationship to the disease. Conduct a brief web search to learn about the ro

Enivronment, which is the probable principal source of nitrates and phospha...

which is the probable principal source of nitrates and phosphate a. the water cycle b. nitrogen fixation c. bacterial decay d. change in environmental temperature e. changes in lig

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd