Animal tissue culture , Biology

Assignment Help:

Animal Tissue Culture:

The term tissue culture refers to the culture of all organs, tissue fragments and dispersed cells on a suitable nutrient medium. It may be divided into (1) cell culture (2) organ culture and basically on the basis of whether the tissue organisation is taken or not. In organ cultures all embryonic organs or small tissue fragments are cultured in vitro in such a manner that they achieve their tissue architecture. In compare, cell cultures are gained either by enzymatic or mechanical dispersed of tissues into particular cells or by spontaneous migration of cells from an explants; they are maintained as attached or as cell suspensions or  monolayers.

Newly isolated cell cultures are called primary cultures; they are typically heterogeneous and slow rising, but are more representative of the tissue of their origin both in properties and cell type. Once a first  culture is subcultured, this given rise to cell lines which can either die after many subcultures (such cell lines are known as finite cell lines) or can continue to grow indefinitely (these are called continuous cell lines. Generally normal tissues give rise to finite cell lines, whereas tumours give rise to continuous cell lines. But there are many examples of continuous cell lines which were imitative from normal tissues and are themselves non-tumorigenic, e.g.3T3 fibroblasts, MDCK dog kidney etc. The growth of continuous cell lines from primary cultures is supposed to involve a mutation which alters their properties as compared to those of finite lines.

 AugmentThe beginning of animal tissue culture may be traced to 1880 when Arnold showed that leucocytes may divide outside the body. Later in the year 1903, Jolly studied the behaviour of animal tissue explants immersed in serum. Lymph or ascites fluid. In the year 1907, Harrison cultured frog tadpole spinal cord in the lymph drop hanging from a cover slip into the cavity on microscopic slide; this is considered  as the turning point. Subsequently in the year 1913, Carrel developed a complicated method for maintaining cultures free from contamination, mainly  by bacteria. Afterwards, suitable culture media were developed and the techniques of cell culture were refined. 


Related Discussions:- Animal tissue culture

Solubility of gases in water, Solubility of Gases in water Most gases w...

Solubility of Gases in water Most gases which are important for biological processes dissolve readily and specially in water. The solubility of any gas in water generally var

Explain the principle of development, Explain the Principle of Development ...

Explain the Principle of Development The final guiding principle is that assessment involves the measure of change, or development, across multiple levels of analysis. Developm

Discuss in detail about the human brain, Discuss in detail about the human ...

Discuss in detail about the human brain  "wiring-up" of our brain begins in early development. When our genetic blueprint largely drives this wiring in womb, a newborn baby's b

Define about the nutrition, Define Nutrition, Diet and Cancer? In this,...

Define Nutrition, Diet and Cancer? In this, we will discuss the common forms of cancer, how they develop, relevant etiological factors, pathological/metabolic changes and other

Zoology, what does ICZN stand for

what does ICZN stand for

How do contraceptive pills generally work, How do contraceptive pills gener...

How do contraceptive pills generally work? Contraceptive pills generally have the hormones estrogen and progesterone. If taken daily from the 4th day after menses the abnormal

Role of cell death, Role of cell death As development of the limb proc...

Role of cell death As development of the limb proceeds waves of death or necrosis of large masses of mesodermal cells occur in certain regions at different stages. This has be

Strobilation - types of asexual reproduction, Strobilation - Types of Asexu...

Strobilation - Types of Asexual Reproduction Strobilation is a type of asexual reproduction in which successive segments are separated off from the body one after other. It is

Define mineral requirements during pregnancy period - iron, Define Mineral ...

Define Mineral Requirements during pregnancy period - Iron? While B 12 and folk acid are needed for the normal erythropoiesis, they must be accompanied by adequate amounts of

Lab safety, Why should you not apply cosmetics while doing a lab experiment...

Why should you not apply cosmetics while doing a lab experiment in class?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd