Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Animal Tissue Culture:
The term tissue culture refers to the culture of all organs, tissue fragments and dispersed cells on a suitable nutrient medium. It may be divided into (1) cell culture (2) organ culture and basically on the basis of whether the tissue organisation is taken or not. In organ cultures all embryonic organs or small tissue fragments are cultured in vitro in such a manner that they achieve their tissue architecture. In compare, cell cultures are gained either by enzymatic or mechanical dispersed of tissues into particular cells or by spontaneous migration of cells from an explants; they are maintained as attached or as cell suspensions or monolayers. Newly isolated cell cultures are called primary cultures; they are typically heterogeneous and slow rising, but are more representative of the tissue of their origin both in properties and cell type. Once a first culture is subcultured, this given rise to cell lines which can either die after many subcultures (such cell lines are known as finite cell lines) or can continue to grow indefinitely (these are called continuous cell lines. Generally normal tissues give rise to finite cell lines, whereas tumours give rise to continuous cell lines. But there are many examples of continuous cell lines which were imitative from normal tissues and are themselves non-tumorigenic, e.g.3T3 fibroblasts, MDCK dog kidney etc. The growth of continuous cell lines from primary cultures is supposed to involve a mutation which alters their properties as compared to those of finite lines.
AugmentThe beginning of animal tissue culture may be traced to 1880 when Arnold showed that leucocytes may divide outside the body. Later in the year 1903, Jolly studied the behaviour of animal tissue explants immersed in serum. Lymph or ascites fluid. In the year 1907, Harrison cultured frog tadpole spinal cord in the lymph drop hanging from a cover slip into the cavity on microscopic slide; this is considered as the turning point. Subsequently in the year 1913, Carrel developed a complicated method for maintaining cultures free from contamination, mainly by bacteria. Afterwards, suitable culture media were developed and the techniques of cell culture were refined.
Q. What are the elements that form the middle ear? What are the names of the three middle ear ossicles that participate in the phonosensitivity? The middle ear is formed by the
Explain Rifamycins-Rifampin Rifamycins - Rifampin, like isoniazid, is potentially hepatotoxic, and gas- trointestinal disturbances, morbilliform rash and thrombocytopenic purpu
Difference between unconditioned and conditioned reflex action- S.NO . 1. UNCONDITIONED It is inborn CONDITIONED
Explain Keshan disease caused by Selenium deficiency? It is a cardiomyopathy (disease of the myocardium, involving heart muscle) that was identified to affect children and wome
Viscosity Viscosity, as you may already know, is associated with fluid flow. It is the internal friction which tends to bring to rest portions of the fluids moving relative to
In some protozoans, food is always ingested at the same place on cell surface, cytostome (cell mouth). Undigested food is removed at a cytoproct, or cell anus. All of this is simil
Myasthenia Gravis Myasthenia gravis (severe muscle weakness) is characterised by muscular fatigue in the wake of very little exercise. It may be apparent after a short period o
Transfer of pollen grains from the anther to the stigma of another flower of the similar plant is known as? Geitonogamy
Q. What is Cholesterol? In its free, unesterified form cholesterol is a major component (together with phospolipid) of cell membranes. Its presence helps to stabilize membrane
What is the central principle of molecular biology?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd