Analysis of metabolic components , Chemistry

Assignment Help:

1. Produce a figure with an appropriate figure legend of your SDS-PAGE gel that could be published in a scientific journal.

  •   Indicate all relevant information, e.g.origin, front, size of the molecular mass markers and casein and whey protein bands. You can use e.g. Power-Point, Photoshop or Illustrator or similar software for his task
  •   Write a concise figure legend with a minimal number of words that allows the reader to understand what he sees. Note: Give some information about the methods but do not repeat all details in the legend!

2. Fill in the table to report your calculated estimates for the molecular masses (kDa) of the proteins in the milk sample. You can enter up to 3 different significant bands that you observed in the respective fractions.

Protein fraction

Estimated molecular mass (kDa)

Casein proteins

 

 

 

Whey proteins

 

 

 

Small molecular mass proteins

 

 

 

3. Draw a graph of absorbance against fraction number for the gel filtration chromatography. Using Excel you will need to create a graph with two axes (one axis on the left for the absorbance of haemoglobin (Abs 400 nm) and one axis on the right for the absorbance of vitamin B12 (Abs 550 nm).

4. Explain in molecular terms the observed movement of the various sized proteins through:

a) the polyacrylamide gel

b) the gel filtration column

5. A cell consists of thousands of individual molecular components. To understand biochemical pathways is often necessary to separate, purify and analyse the molecular constituents of a cell. Name three different biochemical/biophysical properties in which biological molecules differ from each other and think about a corresponding technique that can be exploited to separate and/orcharacterize such metabolites.

Biochemical/Biophysical property

Technique that exploits this property

Example given: different ionic charge

Example given: separation on gel in electric field

 

 

 

 

 

 

6. Consider an individual who had to dash for 500 metres in order to catch a tram that was just about to depart. Indicate thelikely concentration of metabolites that molecular analysis would reveal in the muscle tissue of this person. Increased, decreased and unchanged are describing levels relative to a healthy resting state.

Metabolite

Concentration

(Increased, decreased or unchanged)

acetyl-CoA

 

pyruvate

 

lactate

 

glucose (blood)

 

glucose-6-phosphate

 

EtOH

 

glyceraldehyde-3-phosphate

 

AMP

 

glucose-1-phosphate

 

ephinephrine

 


Related Discussions:- Analysis of metabolic components

Tests of benzaldehyde - aldehydes and ketones, Tests of benzaldehyde - alde...

Tests of benzaldehyde - aldehydes and ketones (a) It forms a white precipitate with   solution. (b)  It creates a yellow precipitate with 2: 4 dinitrophenyl hydrazines. (

Describe about temperature scales, Q. Describe about temperature scales? ...

Q. Describe about temperature scales? The temperature of an entity explains how hot or cold the object is relative to other objects. Scientists utilize two temperature scales-t

Spdf, how to solve the spdf i don''t understand

how to solve the spdf i don''t understand

Important synthetic rubbers: nitrite rubbers , Nitrite rubbers (Gr-A or Bun...

Nitrite rubbers (Gr-A or Buna-N or NBR): Nitrite rubber is prepared by the copolymerization of butadiene and acrylonitrile in emulsion systems. Properties: 1.

Miller indices of the diagonal plane of a cube, Miller indices of the diago...

Miller indices of the diagonal plane of a cube are   (110)

Atom of a chemical element, The atomic weight is the weight of an atom of a...

The atomic weight is the weight of an atom of a chemical element.  For instance, the atomic weight of oxygen is 15.9994 and the atomic weight of hydrogen is 1.0079.  Write a script

Acid-base titrations - types of titrations, Acid-base titrations - Types of...

Acid-base titrations - Types of titrations While the strength of an acid is determined along with the help of a standard solution of base, it is termed as acidimetry. Similarly

Correct ground state electronic configuration chromium atom, The correct gr...

The correct ground state electronic configuration of chromium atom is: (1) [Ar] 3d 5 4s 1                (2)[Ar]3d 4 4s 2      (3) [AR]3d 6 4s 0                (4

Limitations of flame photometry, Limitations of flame photometry: The ...

Limitations of flame photometry: The limitations of the techniques involve the following. The low temperature of the natural gas and air flame limits a method to simply

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd