Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
1. Produce a figure with an appropriate figure legend of your SDS-PAGE gel that could be published in a scientific journal.
2. Fill in the table to report your calculated estimates for the molecular masses (kDa) of the proteins in the milk sample. You can enter up to 3 different significant bands that you observed in the respective fractions.
Protein fraction
Estimated molecular mass (kDa)
Casein proteins
Whey proteins
Small molecular mass proteins
3. Draw a graph of absorbance against fraction number for the gel filtration chromatography. Using Excel you will need to create a graph with two axes (one axis on the left for the absorbance of haemoglobin (Abs 400 nm) and one axis on the right for the absorbance of vitamin B12 (Abs 550 nm).
4. Explain in molecular terms the observed movement of the various sized proteins through:
a) the polyacrylamide gel
b) the gel filtration column
5. A cell consists of thousands of individual molecular components. To understand biochemical pathways is often necessary to separate, purify and analyse the molecular constituents of a cell. Name three different biochemical/biophysical properties in which biological molecules differ from each other and think about a corresponding technique that can be exploited to separate and/orcharacterize such metabolites.
Biochemical/Biophysical property
Technique that exploits this property
Example given: different ionic charge
Example given: separation on gel in electric field
6. Consider an individual who had to dash for 500 metres in order to catch a tram that was just about to depart. Indicate thelikely concentration of metabolites that molecular analysis would reveal in the muscle tissue of this person. Increased, decreased and unchanged are describing levels relative to a healthy resting state.
Metabolite
Concentration
(Increased, decreased or unchanged)
acetyl-CoA
pyruvate
lactate
glucose (blood)
glucose-6-phosphate
EtOH
glyceraldehyde-3-phosphate
AMP
glucose-1-phosphate
ephinephrine
Benzylic halide follows sn1 mechanism always...............the order of halides going sn1 rxn is BENZYL>ALLYL>TERTIARY>SECONDARY>PRIMARY>METHYL beacause of stability, benzyl & ally
Q. Describe about Halide Minerals? Highly electropositive alkali and alkaline earth metals tend to form halide salts, which being soluble in water are washed away into the ocea
Entropy (S) is a molecular 'disorder', or more precisely 'the number of microscopic arrangements of energy possible in a macroscopic sample'. Entropy depends strongly on the state
Formation of proteins-peptide bond: Proteins are made by joining the carboxyl group of one amino acid to the α-amino group of other amino acid. The bond build among two amino a
how did atoms appear
Q. Why we need Sulfuring? Sulfuring is the process which is used to destroy microorganisms and to preserve colour. Fruits after blanching may be dipped into a sulphite solution
Glucose and fructose are epimers so they are differ in their second carbon position and remaining is same so when they react with phenylhydrazeen they give same osazone
concentration on acetic acid
Lithium Fluoride is less soluble in water. But in some ways consider that when Lithium Fluoride dissolve in water, an alkaline solution will find. can u please explain that why thi
caco3
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd