Analysis of metabolic components , Chemistry

Assignment Help:

1. Produce a figure with an appropriate figure legend of your SDS-PAGE gel that could be published in a scientific journal.

  •   Indicate all relevant information, e.g.origin, front, size of the molecular mass markers and casein and whey protein bands. You can use e.g. Power-Point, Photoshop or Illustrator or similar software for his task
  •   Write a concise figure legend with a minimal number of words that allows the reader to understand what he sees. Note: Give some information about the methods but do not repeat all details in the legend!

2. Fill in the table to report your calculated estimates for the molecular masses (kDa) of the proteins in the milk sample. You can enter up to 3 different significant bands that you observed in the respective fractions.

Protein fraction

Estimated molecular mass (kDa)

Casein proteins

 

 

 

Whey proteins

 

 

 

Small molecular mass proteins

 

 

 

3. Draw a graph of absorbance against fraction number for the gel filtration chromatography. Using Excel you will need to create a graph with two axes (one axis on the left for the absorbance of haemoglobin (Abs 400 nm) and one axis on the right for the absorbance of vitamin B12 (Abs 550 nm).

4. Explain in molecular terms the observed movement of the various sized proteins through:

a) the polyacrylamide gel

b) the gel filtration column

5. A cell consists of thousands of individual molecular components. To understand biochemical pathways is often necessary to separate, purify and analyse the molecular constituents of a cell. Name three different biochemical/biophysical properties in which biological molecules differ from each other and think about a corresponding technique that can be exploited to separate and/orcharacterize such metabolites.

Biochemical/Biophysical property

Technique that exploits this property

Example given: different ionic charge

Example given: separation on gel in electric field

 

 

 

 

 

 

6. Consider an individual who had to dash for 500 metres in order to catch a tram that was just about to depart. Indicate thelikely concentration of metabolites that molecular analysis would reveal in the muscle tissue of this person. Increased, decreased and unchanged are describing levels relative to a healthy resting state.

Metabolite

Concentration

(Increased, decreased or unchanged)

acetyl-CoA

 

pyruvate

 

lactate

 

glucose (blood)

 

glucose-6-phosphate

 

EtOH

 

glyceraldehyde-3-phosphate

 

AMP

 

glucose-1-phosphate

 

ephinephrine

 


Related Discussions:- Analysis of metabolic components

Illustration of modelling for process and recipe, Strategy 1 Wheat flo...

Strategy 1 Wheat flour F 3940 g is, mixed with 60g of A, B and C, yielding a ratio of chemicals to flour of 3:197 in all formulations. All mixtures are produced in total amoun

Determine the final pressure and steam condenses, A 15 m 3 tank contains s...

A 15 m 3 tank contains steam at 10 bar and 300 o C. The system is then cooled until the temperature reaches 150 o C . Some of the steam condenses. a) How much heat was transf

Ionisation enthalphy, what is the relevance of half filled and completely f...

what is the relevance of half filled and completely filled orbitals in ionisation enthalphy ditermination

PEPTIDE BONDS AND HYDROLYSIS, I UNDERSTAND THE CONCEPT. I AM CONFUSED ABOUT...

I UNDERSTAND THE CONCEPT. I AM CONFUSED ABOUT A SPECIFIC EQUATION OR FORMULA TO SHOW HOW THE BONDS ARE BROKEN THROUGH HYDROLYSIS.

What do you mean by dative covalent bonds, Q. What do you mean by Dative Co...

Q. What do you mean by Dative Covalent Bonds?Dative Covalent Bonds Ans. Sometimes both electrons in the shared pair come from one atom. This type of bond is called a dativ

Gases, a small 0.600ml bubble forms at the bottom of a lake where the tempe...

a small 0.600ml bubble forms at the bottom of a lake where the temperature is 4.0 degrees Celsius and the pressure is 2.40 atm. What volume will the bubble occupy near the surface

Distillation, Ask question #Minimum 100oo words

Ask question #Minimum 100oo words

Ionic and covalent bonds, Ionic and covalent bonds are present in: (1) ...

Ionic and covalent bonds are present in: (1)  CCl 4                                               (2)  CaCl 2              (3)  NH 4 Cl

Insulating vessel having negligible heat capacity, 2 Kg of ice at -20 degre...

2 Kg of ice at -20 degree C is diverse with 5Kg of water 20 degree C in an insulating vessel having negligible heat capacity. Measure the final mass of water remaining in the con

Explain classification of arches, Explain classification of arches accordin...

Explain classification of arches according to shapes raw a neat sketch of Flemish bond (1 brick wall and 1.5 brick wall thickness ).

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd