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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
Assessment While assessing the patient with rheumatic fever, a detailed history including, problematic environmental factors or recent exposure to streptococcal infection is
Define the first step to envision the pattern of deformation. The first step in the analysis is to envision the pattern of deformation that would accompany such a failure. In t
Using an average wavelength of 450 nm (0.45 um) can you resolve two objects that sit greater than 200nm apart from one another? Use mathematical calculations to justify your answer
functions of arteries in mammals
Secondary, tertiary, and quaternary structures of proteins are spatial structures. Denaturizing is modification in any of these spatial structures that makes the protein deficient
Why should Posterior wall preffered during surgery Posterior wall should not be perforated during surgery. This limits bleeding from the pterygoid plexus or branches of the max
Mitral valve prolapse (MVP) has emerged as a prominent, predisposing structural cardiac abnormality and in adults accounts for 7 to 30 per cent of NVE in cases not related to drug
Explain the Nutrient Deficiency Disorders? Protein Energy Malnutrition (PEM), iron deficiency anaemia (IDA) and vitamin A deficiency (VAD) are still the major public health pro
Q. Are the limbs modified into wings of bats and the wings of birds instance of evolutionary homology or analogy? What about whale fins compared to fish fins? Bird and Bat wing
Transport in Phloem The basic necessities of plants, water, are taken up by the roots. Another purpose served by the roots is to absorb water soluble mineral nutrients from th
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