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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
Q. Complications of diverticular disease? The many complications of the disease include the 'following conditions: • A perforation (hole) in the intestine leading to perito
Describe the events which lead to the formation of (a) identical twins, (b) fraternal twins. (a) Identical twins are derived from the products of a one zygote which separa
Exudate gums Most exudates gums come from compounds produced when the plant is wounded and these substances seal the wound. Most widely used exudate gum is 'gum Arabic' exuded
Glucokinase In the fed state, clearance of blood glucose is mainly by liver via glucokinase. Glucokinase, which is an inducible enzyme, removes most of the blood glucose fr
do you think the social and cultural environment of the 18th and 19th centuries helped or hindered the study of microbiology in particular and science in general
Q. How are the triglycerides made? Triglycerides, oils or fats, are made of one molecule of glycerol bound to three molecules of fatty acids. Hydroxyls of each one of the three
Question 1 Discuss the quantitative methods for the estimation of urinary proteins. What are the instructions to be given to a patient while collecting urine for biochemical exami
What is the meaning of Chelicifore? These feeding mouthparts are found on most, pycnogonads. Though the proboscis is the main feeding structure in these animals when chelicifor
Define Trauma - Nutrition During Stress? The term "trauma" conies from a Greek word which means "a wound" (and or damage or defect). Trauma is a form of shock to the human body
Sample Outline of Child History Questionnaire Basic identifying data: Child's name Date of birth Date of evaluation Person referring for evaluation Per
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