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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
What are cytokinins? Where are they made? Cytokinins are phytohormones active in the promotion of cellular division; they slow down the aging of tissues and act together with a
Define the role of riboflavin in Drug and lipid metabolism? Flavoproteins catalyze dehydrogenation reactions, as well as, hydroxylations, oxidative decarobxylations, deoxygenat
Define Advantages and Disadvantages of Measurement of Cell Mass? Advantage 1. It is a useful technique for estimating fungal and actinomycetes growth. Disadvantages 1.
Explain Unresorbable Barriers - Root Perforation MTA exhabits excellent tissue biocompatible non resorbable barrier and restorative material. It represents an extraord
Define the importance of Iodine in human? Iodine derives the nutritional importance as a constituent of thyroid hormones, 3,5,3',5' tetraiodo-thyronine (thyroxine or T 4 ) and
Triple Fusion The fusion process between the other male gamete and the secondary nucleus follows the same pattern as syngamy. In most plants, the polar nuclei are only partial
Efficiency of feed utilization on complete feeds The complete diet concept is based on group rather than individual feeding and usually involves much fewer changes in diet for
Explain in detail about the optic nerve The optic nerve contains more than one million axons that initiate in the ganglion cell layer of the retina. This structure originatcs a
why does the removal of the extremity of coleoptile prohibit plant growth?
In Chemical Reactions that have a large negative /\Go' a- the total amount of both reactants and products decreases b- the products are less stable than the reactants c- t
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