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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
Posterior Leaflet: In the posterior leaflet a quadrangular excision of the sector involved in the prolapse is done. This may be up to 15-20 per cent of the leaflet. The remaining
Realization of P-D Polarity of Limb Realization of P-D polarity of limb expressed in a specified proximo-distal sequence of differentiation of limb pans is controlled through
What is the difference between ulcerative colitis and Crohn's disease? The differences between the two include: I) Anatomic distribution of the inflammatory process: Crohn'
'Absolute food safety' is the assurance that damage or injury from use of a substance is impossible. Normal 0 false false false EN-US X-NON
Caution for the use of Pipettes - Food Microbiology? (1) Never do pipetting with mouth. (2) For culturing, sterilized pipettes should be used. (3) Never keep pipettes on
Which bone forms the non-moving muscle attachment in (a) the hip joint, (b) the shoulder joint, (c) the ankle joint? (a) The pelvic girdle gives the non
Q. What is Phytates? Phytates are widely distributed in seeds of vegetables, fruits and grains. Unrefined cereals and millets are the richest sources of phytates. Refined or
Q. Explain about Non Insulin Dependent Diabetes Mellitus? Overweight and obese adults are generally afflicted by this type of diabetes. The insulin produced by the pancreas is
What are the main substances transferred from the mother to the fetus through the placenta? And from the fetus to the mother? From the mother to the fetus the major transferre
Explain Tropical Rain Forests in biomes? You probably are well aware of the danger that the world's rain forests are facing in terms of extinction! There are huge numbers of sp
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