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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
How many parts of human body?
Q What are the major morphological features of birds? Birds are animals that present aerodynamic bodies covered with feathers, pneumatic bones, anterior limbs transformed into
Explain factors influencing rs content of foods? 1) Presence of sugar and lipids: Studies so far show that presence or addition of lipids and sugar reduces RS content. 2) Ca
Submerged Stage - Hydrarch This habitat which is now shallower and is richer in nutrients and where light is available up to a certain depth, becomes suitable for the growth o
Define Effect of feeding method on drug availability? The form in which a drug is administered or enters the body can influence its absorption, metabolism or excretion. This be
Explain the Skeletal System? Skeletal System: The support framework, or skeleton, in the human body is composed of mostly hard mineral substances. Unlike crustaceans or insec
Microorganisms Acquired During Transport, Handling and Processing of Raw Foods Microorganisms can come from truck, tanker, food handlers and equipment and utensils used in the
what are the structures of epithelium
Why does having three color receptors (a.k.a. opsins) lead to a more complex color perception than just two?
Explain the Mendel's Laws in genetics? Based on the results of his experiments, Mendel proposed three laws regarding the inheritance of traits: the Law of Segregation, the Law
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