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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
Discuss about the Luria Nebraska procedure The Luria Nebraska procedure involves an age and education correction. It is accomplished by computation of a cutoff score for abnorm
Explain Oxidative phosphorylation Oxidative phosphorylation : Synthesis of ATP from ADP and Pi during the passage of electrons in the respiratory chain.
Regional Specialisation Regional specialisation takes place usually by three processes – 1) Restriction of certain structures to a few segments, for example, gonads are res
green gland in prawn
how does chamaleon change its colour
Ritonavir (RTV, Norvir) Ritonavir is well absorbed from the gastrointestinal tract and at full doses potently inhibits HIV, but due to poor tolerability it is now used mainly
What is Organelles? Organelles : Eukaryotic cells contain various membrane-bound structures called organelles, in contrast to prokaryotes, which lack a definite internal or
Q. Describe Shunts? Detection, localization and quantification of intracardiac shunts are one of the most important exercises in cardiac catheterization. In most cases a prelim
What is Conventional breeding Conventional breeding is a process in which genes for pesticidal traits are introduced into a plant by natural methods, such as cross-pollination
Define Nutrient and Storage protein - biological function of protein? These proteins store amino acids. Ovalbumin is the protein of egg white, employed as an amino acid source
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