Allele-specific ligation, Biology

Assignment Help:

Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.


Related Discussions:- Allele-specific ligation

Which elements contains carbohydrates, (a) Which elements contains Carbohyd...

(a) Which elements contains Carbohydrates (b) Which elements contains Proteins   a) Carbohydrates contain the elements carbon , hydrogen and oxygen. b) Protei

Bovine ephemeral fever, Bovine ephemeral fever Ephemeral fever is commonly...

Bovine ephemeral fever Ephemeral fever is commonly known as 'three-day sickness'. It affects mainly cattle and occasionally sheep in India. Causative agent is a mosquito-borne vir

Explain the term- pedon and polypedon, Explain the term- pedon and polypedo...

Explain the term- pedon and polypedon The characteristics of soil do not change vertically downwards only, there are lateral variations also as soil is a three dimensional body.

Briefly explain associated mitral regurgitation, Explain Associated Mitral ...

Explain Associated Mitral Regurgitation and their role in miscellaneous conditions? Normal 0 false false false EN-IN X-NONE X-NONE

Can you explain nerves, Q. What are nerves? Axons extend all through th...

Q. What are nerves? Axons extend all through the body inside nerves. Nerves are axon-containing structures presenting many axons and covered by connective tissue. The nerves co

Disorders of female reproductive system, DISORDER S OF FEMALE REPRODUCTIVE...

DISORDER S OF FEMALE REPRODUCTIVE SYSTEM - (i)         Sterility (ii )     Menstrual irregularity - This may be amenorrhea (absence of M.C.) or excessive bleeding (

Causes of gastro oesophageal reflux disease, Q. Causes of gastro oesophagea...

Q. Causes of gastro oesophageal reflux disease? GERD may develop due to any of the following reasons: • decreased muscle tone or abnormal relaxation of the LES, • reduced st

What does a two-direction arrow show in a chemical equation, What does a tw...

What does a two-direction arrow show in a chemical equation? The oxygen atoms share two pairs of electrons, as every atom needs two more electrons to fill the orbitals of its o

Illustrate the categories of alloplastic implant materials, Categories of a...

Categories of alloplastic implant materials The entire groups of possible alloplastic implant materials, regardless of their clinical applications fall into the following categ

Explain cis-trans isomers, Explain Cis-trans isomers Atoms or groups a...

Explain Cis-trans isomers Atoms or groups are called  cis or  trans to one another when they project respectively on the same or on opposite sides of a reference plane  identi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd