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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
What is the gross primary production of an ecosystem? How does GPP relate to photosynthesis? Gross primary production of an ecosystem, or GPP, is the amount of organic material
Uses of modified starches in food and confectionery industries Visit a grocery shop and look out for products such as the instant desserts, jelly beans, salad dressings, tomato
Q. Important Herbaria? The herbarium is a place where dried and mounted specimens are stored according to any recognised system of classification. Special attention is paid tow
Ritonavir (RTV, Norvir) Ritonavir is well absorbed from the gastrointestinal tract and at full doses potently inhibits HIV, but due to poor tolerability it is now used mainly
Q. Illustrate Mitral Stenosis and Pregnancy ? Since mitral stenosis is often seen in young women, it is not uncommon to see young women with pregnancy complicated by mitral ste
Explain the Sedimentation - Air Sampling? It is the simplest method, which involves exposing agar plates in air for specified time at a location to be sampled. Microorganisms
Energy Cycle of Living Being Source of energy :All matter locks energy in the from of bonds between component molecules and atoms. This energy is the chemical ener
What are organelles, which carry out autophagy? Autophagy, being the degradation of cytoplasmic components as well as other organelles, is usually done with the lysosomes. The
Photosynthesis Photosynthesis - the process by which plants utilise energy from sunlight to convert carbon dioxide and water for the synthesis of sugar. This sugar can then be
What are the kinds of plant geotropisms? Why do the root and the stem present opposite geotropisms? The kinds of geotropisms are the positive geotropism, that in which the plan
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