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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
Mechanism of Carcinogenesis In the preceding sub-section you learnt that carcinogenesis is a multistep process. Even though cancer may be caused by many agents chemical, p
Define Partition chromatography - basic separation technique? It is a technique in which separation is achieved as a consequence of the relative solubility of a substance in th
Class of Subphylum Uniramia - Pauropoda A small group of uniramians, there are 500 species of Pauropoda explained so far. The minute organisms computing about 1.5 mm in length
All or None Response A very weak stimulus does not result in an action potential. The stimulus must have a certain strength to evoke an excitation. This is the threshold value
Food infection occurs when a pathogen enters the gastrointestinal tract and multiplies. Microorganisms can penetrate into the intestinal mucosa and grow there, or they can pass thr
Explain the Crestal Bone Levels Crestal Bone Levels: Bone levels should be assessed on the mesial and distal surfaces of implants. Any changes between visits should be documen
Explain Measurement of Cell Mass - Microbial Estimation? You may recall reading earlier that filamentous bacteria and moulds cannot be counted satisfactorily by employing plate
Contig: Number of uses, all nouns. The term comes from the shortening of the word 'contiguous'. A 'contig' might refer to the map showing placement of a set of clones which comple
Subphylum Vertebrata : (41,700 species, the vertebrates) Vertebral column of bone or cartilage, head is well developed, heart is ventrally situated dorsal aorta, two pairs of
Define the Spray Drying Method? Spray drying is a unique drying process since it involves both particle formation and drying. It is most suitable for drying of liquid foods suc
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