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Allele-specific ligation is the technique permitting discrimination of two allele at locus by giving two short synthetic oligonucleotides which would bind ajjacent to teach other on amplified DNA fragment, and would be ligated in the presence of DNA ligase; if one of alleles comprising mutation overlapped by 3'-end of one oligonucleotide, its ligation to oligonucleotide bound 3' to it would be prevented; to deduce the identity of the unknown allele, differentially labelled oligonucleotide pairs might be designed for each allele and their ligation efficiency compared in the presence of unknown allele.
Question 1 Write a short note on the following Free Energy Cori cycle Fermentation Pentose phosphate pathway Transamination Allosteric Regulation Qu
Initiation Codon is the codon at which the translation of a polypeptide chain is initiated. This is generally the first AUG triplet in the mRNA molecule from 5' end, where the rib
Protoplasmic - Level of body organization Organisms which are made of just one cell are the simplest and the most primitive creatures called unicellular organisms. Their level
Name a few foods which are best blanched? Yes, green beans, carrots, okra, turnip and cabbage should always be blanched. On the other hand, blanching is not needed for onions,
Define the Fluidized Dryers? Fluidization occurs when a flow of fluid upwards through a bed of particles (ranging from fine powders to particulate foods such as diced carrots)
Diagnostic Procedures Taking Clinical history Urine analysis Blood sample for estimation of blood urea nitrogen Antistrepto lysine (ASO) titer
Q. Can you explain Ventricular Tachycardia? The term is usually reserved for at least four or more beats and modified by the term non sustained. Short runs of non-sustained VT
What is Lysosomes? Lysosomes : Animal and fungal cells contain membrane-bound organelles called lysosomes, which are filled with digestive enzymes. These digestive enzyme
What is the location of the salivary glands in humans? There are 6 major salivary glands and they are located one in every parotid gland, two beneath the mandibles (submandibul
Homo sapiens first appeared in the fossil record between 200,000 to 300,000 years ago, The fossils were the Swanscomb man from England and Steinheim man from Germany. a) The Swa
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