Alkaline phosphatase, Biology

Assignment Help:

Alkaline Phosphatase is an enzyme which catalyzes the hydrolysis of the phosphomonoesters of the 5' nucleotides. It is used to dephosphorylate (remove phosphate groups from) the 5' ends of DNA or RNA molecules, to facilitate 5' end-labeling with the 32P added back by T4 polynucleotide kinase; or to dephosphorylate the 5' ends of DNA molecules to prevent unwanted ligation reactions during cloning.


Related Discussions:- Alkaline phosphatase

Spermeiogenesis / spermetoleosis, SPERMEIOGENESI S / SPERMETOLEOSIS It...

SPERMEIOGENESI S / SPERMETOLEOSIS It is the gradual differentiation of a stationary rounded and undifferentiated spermatid into an active and motile sperm. The spermatids f

How to calculate fractional shbrtening, Q. How to calculate Fractional Shbr...

Q. How to calculate Fractional Shbrtening? With the cursor- beam cutting the left ventricle just beyond the tips of mitral valve in an adequate parasternal long axis view,

O-linked oligosaccharides , Most proteins made by ribosomes on the RER (rou...

Most proteins made by ribosomes on the RER (rough endoplasmic reticulum) are glycoproteins, which is they hold short chains of carbohydrates (oligosaccharides) covalently related t

What is the kind of life cycle present in pteridophytes, Q. What is the kin...

Q. What is the kind of life cycle present in pteridophytes? Like all plants pteridophytes present diplobiontic (alternation of metagenesis or generations,) life cycle.

Determine reagents for determination of protein content, Determine Reagents...

Determine Reagents for Determination of Protein Content Using Biuret Method? Collect the following reagents to conduct the experiment: 1. 0.2 N NaOH solution - Dissolve 8 g

What is the function of lh in the menstrual cycle, What is the relationship...

What is the relationship between the estrogen level and the LH level in the menstrual cycle? What is the function of LH in the menstrual cycle and when does its blood concentration

Disadvantage of a monoculture, (a)What do you understand by the term 'monoc...

(a)What do you understand by the term 'monoculture'? (b) What is one disadvantage of a monoculture?   a) 'Monoculture' is the term applied to the growing

Define the skeletal muscle, Define the Skeletal muscle A. Binding of ca...

Define the Skeletal muscle A. Binding of calcium ion to its receptor site on the actin molecule blocks the attachment of the head of the tropomyosin molecule to its binding sit

How do you determine if a molecule is polar or non-polar, How do you determ...

How do you determine if a molecule is polar or non-polar? A polar molecule is a molecule that has a net dipole moment because of its having unsymmetrical polar bonds

Pullorum disease (bacillary white diarrhea), P u l l orum disease (baci...

P u l l orum disease (bacillary white diarrhea) This disease is caused by poultry-adapted strain of Salmonella genus- Salmonella Pullorum and considered an important hatc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd