Alkaline phosphatase, Biology

Assignment Help:

Alkaline Phosphatase is an enzyme which catalyzes the hydrolysis of the phosphomonoesters of the 5' nucleotides. It is used to dephosphorylate (remove phosphate groups from) the 5' ends of DNA or RNA molecules, to facilitate 5' end-labeling with the 32P added back by T4 polynucleotide kinase; or to dephosphorylate the 5' ends of DNA molecules to prevent unwanted ligation reactions during cloning.


Related Discussions:- Alkaline phosphatase

Explain the application of the principles listed, Observe several individua...

Observe several individuals as they run. look for the application of the principles listed. Which are violated and how. The application of the principles The law of inertia

How is the fluid that fills the nucleus called, The aqueous fluid that fill...

The aqueous fluid that fills the nuclear region is known as karyolymph, or nucleoplasm. In the fluid there are proteins, enzymes and other significant substances for the nuclear me

Signal for ribosome to begin translating the transcript, Which of the follo...

Which of the following serves as a signal for the ribosome to begin translating the transcript? A. Addition of the poly a tail to the 3' end of the transcript B. Deamination

Describe different animal groups, Describe different animal groups a) C...

Describe different animal groups a) Could  comprehensively  compare  and  contrast  the  behaviours  exhibited  by  social animals rather than describe different animal groups

The dna revolution, Unprecedental clarity has come to our understanding of ...

Unprecedental clarity has come to our understanding of genetic differences by the analysis of DNA sequences. It is not surprising in which the latest DNA technologies are leading t

State the term - neuropsychological examination, State the term - Neuropsyc...

State the term - Neuropsychological Examination For a neuropsychological examination a battery of tests administered should include, at a minimum: Intelligence Tests

How are the epithelial tissues classified, Q. How are the epithelial tissue...

Q. How are the epithelial tissues classified? The epithelial tissues are classified according to the shape of the cells that form it epithelial cells may be columnar, cuboidal,

Illustrate goal of modern dentistry, Q. Illustrate goal of modern dentistry...

Q. Illustrate goal of modern dentistry? The goal of modern dentistry is to restore the patient to normal contour, comfort, function, esthetics, speech and health regardless of

Extinction of a plant species, How could humans be disadvantaged by the ext...

How could humans be disadvantaged by the extinction of a plant species? The loss of a plant species may deprive humans of any beneficial substances (e.g. drugs) that the specie

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd