Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

Describe Fatty acids, In chemistry and particularly in biochemistry the fat...

In chemistry and particularly in biochemistry the fatty acid is a carboxylic acid with a long aliphatic tail (chain) that is either saturated / unsaturated. Many obviously occurrin

The cell, how autophagy help in converting a tadpole larva into adult amphi...

how autophagy help in converting a tadpole larva into adult amphibian

Implantation - pre-embryonic development, Implantation - Pre-Embryonic Deve...

Implantation - Pre-Embryonic Development After entering the uterus and formation of ICM, the blastocyst starts to embed in the endometrium of the uterine wall. By one week aft

Properties of pyridoxine, Properties of pyridoxine a)  It forms white, ...

Properties of pyridoxine a)  It forms white, odourless  crystals. b)  The compound is readily soluble  in water. c)  When  a neutral or alkaline solution of pyridoxine is

Dna replication at biochemical level, Although there is much talk in the ne...

Although there is much talk in the news about stem cell research, the public and policymakers need to understand how basic body cells work to transmit information and replicate to

Combination of meat products, C o mbination of meat products Combinin...

C o mbination of meat products Combining meats and byproducts of different species to produce value added products results in complementation and supplementation of their qua

Define the fluidized dryers, Define the Fluidized Dryers? Fluidization ...

Define the Fluidized Dryers? Fluidization occurs when a flow of fluid upwards through a bed of particles (ranging from fine powders to particulate foods such as diced carrots)

Describe methanogen archaebacteria and thermoacidophile, Q What are halophi...

Q What are halophile, methanogen archaebacteria and thermoacidophile? There are three peculiar types of archaebacteria the halophile archaebacteria only survive in salt-rich en

Define risk assessment, Define Risk assessment Risk  assessment  has  ...

Define Risk assessment Risk  assessment  has  been  described  as  the process  of  organizing  information concerning a particular  risk in  a systematic way  so  that the dec

Antinutritional factors, Antinutritional factors Unconventional feed resou...

Antinutritional factors Unconventional feed resources including many agriculture by-products are rich in  antinutrients and toxic constituents, and tannins being the most widely o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd