Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

Explain amantadine, Amantadine Treatment with oral amantadine or rimant...

Amantadine Treatment with oral amantadine or rimantadine begun within 48 hours after the onset of illness decreases the duration of fever and symptoms by about 1 day. Whether t

How much fats should be taken for management of obesity, How much Fats shou...

How much Fats should be taken for management of obesity? Fats, being a concentrated source of energy need to be restricted. Excess dietary fat promotes much more weight gain th

Animal population, what are the characteristics of animal population

what are the characteristics of animal population

Explain sodium, Explain Sodium Sodium (Nu)  :  In  sodium-restricted di...

Explain Sodium Sodium (Nu)  :  In  sodium-restricted diets, no salt  is added to the diet which still provides approximately 50 mmoL Na. Foods containing high Na content must b

What are the near point and the far point of the vision, What are the near ...

What are the near point and the far point of the vision? The near point is the closest distance among an object and the eye that makes possible the formed image to be focused,

Determine the food sources of fluoride, Determine the Food Sources of Fluor...

Determine the Food Sources of Fluoride? The major source of fluoride in most diets is water, with foods providing only about 25% of total intake. These include tea and marine f

Autotomy and regeneration, Autotomy and Regeneration Shedding of body...

Autotomy and Regeneration Shedding of body parts in self-defense to avert the attention of the predator-enemy or in any other emergency is a type of autotomy (auto: self, tom

Variations, VARI A TION S - Defined as "dissimilarities of featur...

VARI A TION S - Defined as "dissimilarities of features among members of the same species". Offsprings of same parent are different and also differ from their parent.

Levels of prevention, Family Centred Activities to Ensure Harmonious Relati...

Family Centred Activities to Ensure Harmonious Relationship: Between various members of  the family attitudes of mutual trust, love and respect for one another need  to be  fo

What is lasting form in gametophyte-pteridophytes-sporophyte, What is the l...

What is the lasting form in the gametophyte, pteridophytes or the sporophyte? How can it be compared to bryophytes? The lasting form in pteridophytes is the diploid (2n) sporop

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd