Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

Explain catastrophism, Catastrophism Once-popular belief which events in t...

Catastrophism Once-popular belief which events in the history of earth had occurred in the past a sudden events and by processes different from those operating today. The periods

Relevance of food packaging, Q. Relevance of food packaging? To unders...

Q. Relevance of food packaging? To understand the relevance of food packaging, it is necessary for us to understand how spoilage reduces the availability of food. In the deve

Describe the monomer unit of a protein, Describe the monomer unit of a prot...

Describe the monomer unit of a protein and how monomer units are assembled into peptides.

What is folic acid, What is folic acid? Why is the anemia caused by deficie...

What is folic acid? Why is the anemia caused by deficiency of folic acid known as megaloblastic anemia? The folic acid (when ionized it is known as folate) is a coenzyme that

What are the factors to an actual shift in the supply curve, What are the f...

What are the factors to an actual shift in the supply curve? Factors which cause an actual shift in the supply curve are described in below: When prices rise or fall this wo

Pathogenesis of acute rheumatic fever, You have learnt about the epidemiolo...

You have learnt about the epidemiology, etiology and pathogenesis of acute rheumatic fever. Revised Jones criteria, which have major and minor criteria, are helping to diagnosis of

Explain coronary heart disease, Apart from any inherited tendency towards c...

Apart from any inherited tendency towards coronary heart disease, what are thought to be the four major risk factors?  The four major risk factors for coronary heart disease ar

Photoorganoheterotrophs, photoorganoheterotrophs why don''t use light for p...

photoorganoheterotrophs why don''t use light for photosynthesis?

Which is better to grow plants in rock sand or soil, Which is better to gro...

Which is better to grow plants in Rock sand or soil? When we grew plants inside, with there is no wind, and the plants in the rocks grew improved than the plants that were grow

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd