Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

Define briefly about the conjugated proteins, Define briefly about the Conj...

Define briefly about the Conjugated Proteins? Conjugated proteins are made up of simple proteins combined with a non- proteinous substance. The non-proteinous substance is know

How does the intensity of facilitated diffusion differ, Q. How does the int...

Q. How does the intensity of facilitated diffusion differ in relation to the concentration of the moved substance? What is limiting factor? Like simple diffusion facilitated di

What is the vector of chagas disease, What is the vector of Chagas' disease...

What is the vector of Chagas' disease? How is the disease transmitted? The vector of Chagas' disease is its middle host, a triatomine bug. The major species is Triatoma infesta

Define transcription phase of gene expression process, Define Transcription...

Define Transcription Phase of Gene Expression Process? Transcription is the enzyme synthesis of RNA on a DNA template. This is the first stage in the overall process of gene ex

Find out a mutation in the aldolase b gene, Hereditary fructose interolance...

Hereditary fructose interolance is a rare recessive disorder that is caused by a mutation in the aldolase B gene. As a result of this disorder individuals may have all of the follo

Pulmonary valvotomy with infundibular resection, Pulmonary Valvotomy with I...

Pulmonary Valvotomy with Infundibular Resection :  Infundibular obstruction in cases of pulmonary valvar stenosis could be primary or secondary. 11' this obstruction is signi

Why fish and sea foods are important for human body, Why Fish and Sea Foods...

Why Fish and Sea Foods are important for human Body? They are rich sources of proteins (20-25%) of a high biological value. Dry fishes contain more (60%) proteins since most of

Explain a reason these cells are used instead of outer body, The cells lini...

The cells lining the inside of the cheek are frequently removed for making observations of basic cell structure. The cells are from stratified squamous epithelium. Explain a reason

Fats, definition and explanation of fats

definition and explanation of fats

What we need undergloves, Q. What we need Undergloves? These are a cott...

Q. What we need Undergloves? These are a cotton glove worn under the treatment glove for those operators experiencing discomfort to the skin on the hands. These must be replace

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd