Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

Stereoisomers , Glyceraldehyde has a one asymmetric carbon atom the central...

Glyceraldehyde has a one asymmetric carbon atom the central one and so two stereoisomers  which is also known as optical isomers are possible, which is two forms of glyceraldehyde,

Why there is more species biodiversity in tropical latitudes, a) Giving two...

a) Giving two reasons define why there is more species biodiversity in tropical latitudes than in temperate ones. b) Name an upload drug and its source plant. How does the dr

Water absorption and transpiration, Water Absorption and Transpiration ...

Water Absorption and Transpiration The rate of water absorption is controlled by the rate of transpiration. A high water potential in the atmosphere would reduce water loss fr

What percentage of sons will show recessive phenotype, A woman who is heter...

A woman who is heterozygous for a particular X-linked recessive trait marries a phenotypically normal man. What percentage of their sons will show the recessive phenotype?

Differences of transposable elements in e. coli, Discuss the similarities a...

Discuss the similarities and differences of transposable elements in E. coli, yeast, plants, and Drosophila.

Types of community, Types of Community On the basis of size and degree ...

Types of Community On the basis of size and degree of relative independence communities may be divided into two types: i) Major Community: These are large-sized, well org

What does the formula dpd = sf - tp mean, What does the formula DPD = SF - ...

What does the formula DPD = SF - TP mean? DPD is the abbreviation of diffusion pressure shortage, SF (suction force) is the vacuolar osmotic pressure and TP is the turgor press

What is the role of platelet plug formation in hemostasis, What is the role...

What is the role of platelet plug formation in hemostasis? What events occur during platelet plug formation? Describe the circumstances that may induce inappropriate platelet plug

Explain why exact replication of dna is necessary, Figure  shows part of a ...

Figure  shows part of a DNA molecule (a) (i) What do the dotted lines in Figure represent?  (ii) Name J to M. J.............................. K..............................

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd