Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

Explain the pour plate method, Explain the Pour Plate Method Isolated c...

Explain the Pour Plate Method Isolated colonies can also be obtained by pour plate method. The method involves mixing of small volume of microbial suspension with molten nutrie

Describe oxidative stress in new cardio-vascular, Describe Oxidative Stress...

Describe Oxidative Stress in New Cardio-vascular Risk Factors ? In the risk conferred by LDL cholesterol, the oxidative modification of LDL plays a central role, because it is

Chlamydiosis-pathogenesis, Pathogenesis The mode of intracellular repl...

Pathogenesis The mode of intracellular replication of chlamydial agents is investigated morphologically and cytochemically with both light and electron microscope. It is estab

Composed of proteins and dna, Chromosomes, colored bodies, are composed of ...

Chromosomes, colored bodies, are composed of proteins and DNA; so are chromatids. At metaphase I there are 46 chromosomesaligned in pairs along the human cells equatorial plate. At

What about whale fins compared to fish fins, Q. Are the limbs modified into...

Q. Are the limbs modified into wings of bats and the wings of birds instance of evolutionary homology or analogy? What about whale fins compared to fish fins? Bird and Bat wing

Define swollen and tender joints and haemorrhage, Define Swollen and Tender...

Define Swollen and Tender Joints and haemorrhage in various Tissues? Haemorrhages occur deep in muscle, particularly in calf, thigh, buttocks and forearm, causing pain in surro

Describe different animal groups, Describe different animal groups a) C...

Describe different animal groups a) Could  comprehensively  compare  and  contrast  the  behaviours  exhibited  by  social animals rather than describe different animal groups

What are bacteriophages, What are bacteriophages? Bacteriophages are vi...

What are bacteriophages? Bacteriophages are viruses specialized in parasitism of bacteria. They are used in genetic engineering as molecular cloning vehicles to insert recombin

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd