Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

Ordinary share capital, Ordinary share capital This type of Equity fi...

Ordinary share capital This type of Equity finance is raised from the public from the sale of ordinary shares to the shareholders. This finance is presented to limited compan

Show the value of embryology, Q. Show the value of embryology? The valu...

Q. Show the value of embryology? The value of embryology in solving taxonomic problems can be appreciated by studying some specific cases. Amongst the classical examples cited

Explain terms of osmosis, A red blood cell has an internal salt concentrati...

A red blood cell has an internal salt concentration of 150mM. The cell is placed in a beaker of 500mM salt. (a) Assuming that the cell membrane is permeable to water but not to

How to identify the monomer or molecular components, Please help with the f...

Please help with the following: For each of the four macromolecules carbs, lipids, proteins and nucleic acids, please identify the monomer or molecular components and name the b

Recovery of by-products: coal, Recovery of tar: the coke oven gas coming o...

Recovery of tar: the coke oven gas coming out from ovens is passed through a tower where liquid ammonia is sprayed. The tar and dust are removed and then recovered. Recover of

Describe the term physiotherapy, Describe the term Physiotherapy? Patie...

Describe the term Physiotherapy? Patients who are sedated, intubated and ventilated are not able to deep breathe, cough and clear secretions. Hence, it is important to do chest

What is mass transportation across the cell membrane, Mass transportation i...

Mass transportation is the access or the exiting of substances in or from the cell engulfed by portions of membrane. The fusion of internal substance-having membranous vesicles wit

Filaments of a sarcomere in a skeletal muscle is decreasing, When the overl...

When the overlap between the thin and thick filaments of a sarcomere in a skeletal muscle is decreasing, the A. total length of the I band minus the length of the H zone is dec

Explain about natural pigments, Explain about natural pigments There ha...

Explain about natural pigments There has been an extensive search of the microbial, plant and animal kingdoms for pigments that possess both high tinctorial power/strength (a m

Phylum Protozoa, salient features of phylum Protozoa

salient features of phylum Protozoa

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd