Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

To show the respiration of a plant, To show the respiration of a plant ...

To show the respiration of a plant Place the plant in a test tube held in a weighted wooden block. Put this in a bowl having lime water and cover the plant with a jar. Keep the

Sporozoans – protozoan, Sporozoans – Protozoan Sporozoans of the genus...

Sporozoans – Protozoan Sporozoans of the genus Plasmodium are responsible for causing a serious human disease, malaria. They are among, the best known parasites which live in

Illustrate the thin and think filaments in the muscle fiber, Illustrate the...

Illustrate the thin and think filaments in the muscle fiber A healthy skeletal muscle fiber is isolated and has no external forces on it.  It has normal intracellular levels of

What is prophylaxis, What is prophylaxis? Prophylaxis are measures take...

What is prophylaxis? Prophylaxis are measures taken to stop diseases. For instance, the use of condoms in sexual relations is a prophylaxis against contamination by agents that

Aplastic anaemia, Aplastic Anaemia   Aplastic anaemia is a condition in...

Aplastic Anaemia   Aplastic anaemia is a condition in which production of red cells in the bone marrow is reduced. It involves failure to produce adequate red cells, white cell

Delimitation and limitation, Delimitation and Limitation: There may  b...

Delimitation and Limitation: There may  be many  aspects of the problem that need  to be  explored, but  it  is difficult to  cover all aspects in  a single research study bec

Show the ph range of natural honey, Q. Show the pH range of natural honey? ...

Q. Show the pH range of natural honey? The pH of natural honey ranges from 3.4 to 6.1. Acidity of honey is primarily due to presence of acids such as gluconic acid, pyruvic a

Define the blood coagulation - function of vitamin k, Define the Blood coag...

Define the Blood coagulation - Function of Vitamin K? The primary function of vitamin K in the body is in the maintenance of normal blood coagulation. The vitamin K-dependent c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd