Agarose gel electrophoresis, Biology

Assignment Help:

Agarose gel electrophoresis is the method to analyze the size of the DNA (or RNA) fragments. In the presence of an electric field, bigger fragments of DNA move through a gel slower than the smaller ones, producing different migrating "bands". Generally, these are visualized by soaking the gel in a dye (ethidium bromide) which makes the DNA fluoresce under UV light. This is the gel of choice for the DNA or RNA in the range of thousands of bases in length, or up to 1 megabase if you are using pulsed field gel electrophoresis. 


Related Discussions:- Agarose gel electrophoresis

Explain catastrophism, Catastrophism Once-popular belief which events in t...

Catastrophism Once-popular belief which events in the history of earth had occurred in the past a sudden events and by processes different from those operating today. The periods

Cerebral palsy, Cerebral Palsy Cerebral palsy is a non-progressive neu...

Cerebral Palsy Cerebral palsy is a non-progressive neuromuscular disorder characterised by disorder of strength, muscle control, posture or movement due to brain injury, befor

Aesthetic and recreational significance - wildlife, Aesthetic and Recreatio...

Aesthetic and Recreational Significance - Wildlife Many wild species are a source of beauty, wonder; joy, and recreational pleasure for large number of people. Observing leave

Stored metabolites - seed and fruit, Stored Metabolites - Seed and Fruit ...

Stored Metabolites - Seed and Fruit In a large majority of seeds food is stored in the cells of the endosperm. In coconut, wheat and castor bean for example, it is the endospe

What is polymerase chain reactionin genetics, What is Polymerase Chain Reac...

What is Polymerase Chain Reactionin genetics? A great advance in sequencing and cloning DNA is the polymerase chain reaction, devised by the American biochemist Kary Mullis in

Compute how much of the absorbance of the bsa, For a sample containing both...

For a sample containing both protein and nucleic acid, the absorbance measured at a particular wavelength will be the sum of contributions from both types of molecule. Using the pr

Basics of echocardiography, Echocardiography is one of the -most frequent...

Echocardiography is one of the -most frequently used imaging modalities for diagnosing cardiovascular diseases. It i's versatile and is applicable in the entire spectrum of

LIFE, What is life?

What is life?

Explain about the essential fat in humans, Explain about the essential fat ...

Explain about the essential fat in Humans? For the body to function normally, certain amount of body fat is required. This is called essential fat. For women, average amount of

Explain casein, Explain Casein Casein is an important example of protei...

Explain Casein Casein is an important example of protein, which can be boiled without apparent change in stability. The exceptional stability of casein makes it possible to boi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd