You require to make the stock solution of medium for the

Assignment Help Chemistry
Reference no: EM13468950

You are given a stock solution of EDTA at a concentration of 5mM, how would you dilution of EDTA with a final concentration of 5µM in a final volume of 250mL?  You need to prepare a stock solution of medium for your culture cells, which usually includes liquid salt solution and bovine serum. Your liquid salt solution is supplied in a 10X concentration, and you need to dilute it to 1X for use. You also need to add 100% fetal bovine serum for a final concentration of 10%. How would you make up 5 liters of this culture media using water as your solvent?

Reference no: EM13468950

Questions Cloud

The method of creating and maintaining a website usually : question 1 the process of creating and maintaining a website typically involves both development and production
Utilize the internet to research recent investigations : utilize the internet to research recent investigations court cases new technological developments partnerships or other
What concentration must you dilute stock to so that you : you have a 1mm stock solution of arsenic to treat culture cells in a liquid medium. you need to treat your cells with
Assume that you have an array of baseball scores type : suppose that you have an array of baseball scores type integer called scores. the values in the array are ordered from
You require to make the stock solution of medium for the : you are given a stock solution of edta at a concentration of 5mm how would you dilution of edta with a final
The molecular weight of anhydrous calcium chloride is 11099 : the molecular weight of anhydrous calcium chloride is 110.99. how many grams do you need for 100 ml of 50 mm calcium
The molecular weight of sodium hydroxide is 4000 how many : the molecular weight of sodium hydroxide is 40.00. how many grams would you need for 150 ml of a 1mm solution? a buffer
Explain a minimum of three 3 heuristics to optimize : your supervisors and customers are very impressed with the database you have put together. however there is still that
To make up the solution of phosphate buffered saline pbs : to make up a solution of phosphate buffered saline pbs you need 5mm na2hpo4 anhydrous fw 141.96 0.15 m nacl fw 58.44

Reviews

Write a Review

Chemistry Questions & Answers

  Explainbalanced thermochemical equation and enthalpy diagram

Balanced thermochemical equation and enthalpy diagram, The decomposition of zinc carbonate, ZnCO3(s), into zinc oxide, ZnO(s), and CO2(g) at a constant pressure requires the addition of 71.5kJ of heat per mole of ZnCO3.

  Explain the preparation of a tris buffer

Describe the preparation of a 1.50 M, pH 8.0 tris buffer (500 mL) using tris base (MW= 121.1 g/mol) and 3.0 M HCl. pKa of tris is 8.3

  How to calculate the solubility of cubr

Copper(I) ions in aqueous solution react with NH3 according to, Cu+ + 2 NH3 Cu(NH3)2+ Kf = 6.3x10^10 M-2 Calculate the solubility of CuBr (Ksp = 5.3x10^-9) in a solution in which the equilibrium concentration of NH3 is

  Compute the ratio of the concentration

Compute the ratio of the concentration of H 2 CO 3 to that of the HCO 3- if The pH of a bicarbonate-carbonic acid buffer is 8.50

  Compute the dissociation constant ka of ha

When the substance is dissolved in a 1.20x10-3 M solution of HA, the catalyzed rate constant was found to be 3.20x10-5. Calculate the dissociation constant, Ka, of HA.

  Compute the molar solubility of caco3

Calculate the molar solubility of CaCO3 ( Ksp = 4.5*10-9) neglecting the acid-base character

  Explain 3-methyl-2-butanone

3-methylbut-1-ene ---->3-methyl-2-butanone. For the reagents I put, HBr, Tert butoxide, O3 and dms. I was wondering about the O3 and dms, im not sure if that part is right.

  Explain what is the molarity of a sodium hydroxide

Sulfamic acid is a primary standard that can be used to standardize NaOH. What is the molarity of a sodium hydroxide solution

  How many grams of nh3 would be produced

A student in lab has 2.00 g of pure N2 in 0.360 g pure H2. The reaction happens following this equation:

  Define electron acceptor for denitrification

Match each item in Column A with the corresponding item in Column B regarding the processes of nitrification and denitrification: Column A Column B a. NH4+ i. Electron acceptor for denitrification

  How many pills does the patient need to take

A patient needs to take 625 mg of ibuprofen twice daily. The pills in the bottle are each 250. mg. How many pills does the patient need to take each time she wants the 625 mg dosage? Assume that these pills are scored and can be cut in half if ne..

  How many molecules of hcl will we form

If we react 116 molecules of TiCl4 with water according to the equation  TiCl4 + 2 H2O -> TiO2 + 4 HCl how many molecules of HCl will we form?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd