Reference no: EM133141436

Question:

First:

1. You will be conducting a mechanistic investigation of the condensation reaction shown in (J. Am. Chem. Soc. 2020, 142, 5097) (Paper #1). Due to its conceptual similarity to native chemical ligation (NCL),

2. please write a literature review on Mechanistic Study and Understanding of Native Chemical Ligation and Related Reactions. (This is a tentative title) Feel free to modify it to suit your taste. NCL has been studied for many years;

Second: You will find much information in (Chemical Reviews 2019, 119, 7328) (Paper #2). helps you In the review,

1. It would help if you focused on highlighting the methods used to elucidate the reaction mechanism of NCL and related reactions and the mechanistic picture derived from the experimental and computational data.

2. For the latter, information such as the fundamental steps involved: * The rate law of the reaction. * The nature of the intermediate. * The rate/yield dependence on the substrate structure, solvents, and conditions should be included.

Third: Although not included in the Chemical Reviews, the reaction class is shown in (Angew. Chem. Int. Ed. 2009, 48, 9658)(Paper #3) should also be included in the review.

Paper 1: Condensation of 2-((Alkylthio)(aryl)methylene)malononitrile with 1,2-Aminothiol as a Novel Bioorthogonal Reaction for Site-Specific
Protein Modification and Peptide Cyclization

Paper 2: Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations

Paper 3: Protein Labeling - A Biocompatible Condensation Reaction for the Labeling of Terminal Cysteine Residues on Proteins