Why is it important to use positive and negative controls

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Reference no: EM133635322

Question: Define the following terms: Epidemiology Incidence Aerosol ELISA Primary antibody Secondary antibody Disease can be spread through three primary methods: contact transmission, vehicle transmission, or by vector transmission. Describe each mode of transmission below: Contact transmission Vehicle transmission Vector transmission What is COVID-19? What causes COVID-19? How is COVID-19 transmitted

1. What are the medical applications of an ELISA test?

2. Why is it important to use positive and negative controls?

3. Why is it important to perform the assay in triplicate (i.e., 3 wells for each sample)? Your name, # Partner 1 name, # Partner 2 name, # Partner 3 name, # Were you + or -?

4. Why is it important to wash the wells after every step?

5. What happened in each test well? In the wells depicted below, diagram a positive ELISA and a negative ELISA. Include: infectious agent (antigen), primary antibody, enzyme-linked secondary antibody, substrate, and color. Positive ELISA Negative ELISA

6. Did your original sample contain the antigen?

7. How many of the original samples contained the antigen? By the end, what percentage of the class was "infected"

Reference no: EM133635322

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