What the product of the complete hydrolysis contained

Reference no: EM13157270

An oligomeric protein has a molecular weight of 415000. A 740 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger\'s reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCl. The product of the complete hydrolysis contained 1.8 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the ?-amino groups of other amino acids could be found How many polypeptide chains are in this protein?

Reference no: EM13157270

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