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Your first project in your new lab is to purify the protein Rock Band (RB) which is known to be phosphorylated by the kinase Heavy Metal Drummer (HMD). As the new kid on the block, you are tasked with purifying tons of HMD for the lab to use. You first attempt to purify the kinase using ion-exchange chromatography. You scour PubMed and determine that the isoelectric point (pI) of HMD is pH 5.5.
(i) What should the pH of your buffer be if you wanted to bind HMD to an anionexchange column? Why?
(ii) What should the pH of your buffer be if you want to bind HMD to a cation-exchange column? Why?
(iii) What potential issues might arise if the pH of your buffer is too close to the pI of your protein of interest? Why might an extreme buffer pH (< 6 or > 9) be unsuitable?
During this process, 346 J of work was done by the system and its internal energy 9185 J. What is the specific heat of the gas?
The reaction AB-> A+b is second order in AB and has a rate constant of 0.0245 M^-1 S^-1 at 25.0 degree celcuis . A reaction vessel initially contains 250.0 mL of 0.108 M AB which is allowed to react to form the gaseous product.
Heat of Combustion Calculation, Calculate the heat of combustion of cyclohexatriene using the bond energies for C=C,C-C, and CH provided below
Find the formal concentration of the acid in each phase when 100 mL of 0.10 M aqueous acid is extracted with 35 mL of octanol at (a) pH 4.00 and (b) pH 9.00.
What salt is produced in each of the following neutralization reactions?
a student used a pH probe that was not properly calibrated. the probe reads the pH one unit higher than it should be. what would the calculated Ka value of the unknown acid
One of the emission spectral lines for Be3+ has a wavelength of 253.4 nm for an electronic transmission that begins in the state with n = 5.
At 373 K, Keq = 2.39 for the following reaction: SO2Cl2(g) = SO2(g) + Cl2(g) In an equilibrium mixture of the three gases, the partial pressures of SO2Cl2 and SO2 are 3.31 atm and 1.59 atm, respectively.
What is the magnitude of the electric field amplitude associated with the beam and the corresponding photon density per cm3?
How much heat is evolved in converting 1.00 mol of steam at 145°C to ice at -50.0°C? The heat capacity of steam is 2.01J/g?°C and of ice is 2.09J/g?°C.
What is the the molar composition of the products when a stream of CH4 is combusted with 5% excess air if you have 1600 moles per hour of methane being fed into the reactor
Construct a spontaneous voltaic cell using the following materials: A Ni(s) electrode, a copper electrode, a 1.0 M Ni(NO3) (aq) solution and a 1.0 M CuSO4 (aq) solution.
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