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1. What part of the Boil buffer helps "break open" the bacterial cells?
2. What is the difference between bacterial genomic DNA and plasmid DNA that results in the precipitation of genomic DNA with proteins, leaving the plasmid in the supernatant?
3. Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end "sticky" and why is this especially useful for inserting DNA fragments into plasmid DNA?
4. What specific property of DNA allows it to be seperated by size using a gel electrophoresis system?
The seeds in bush bean pods are each the product of an independent fertilization event. Green seed color is dominant to white seed color in bush beans.
Would you expect any differences in the sizes of the inserts in the two libraries? in brief explain.
Animals can be classified according to their mode of thermoregulation. Sort the animals below, indicating their likely thermoregulatory strategy. Note that poikilotherms are sometimes referred to as heterotherms.
The overall reaction of aerobic respiration of glucose is summarized as.
Several traits are affected by the environment, but also have a genetic component. How can we partition the sources of variation? Write a formula for this.
You start to have a scratchy feeling in the back of your throat. After a few days it hurts to swallow, so you go to doctor. The doctor tells you that you have strep throat.
In Drosophila, the map directions of genes are given in map units numbering from one end of a chromosome to the other. The X chromosome of Drosophila is 66 m.u. long.
Assume y ou wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volume.
Think about a population in which the frequency of allele A is p=0.7 and the frequency of allele a is q=0.3, and where the alleles are codominant.
Suppose when warming of the Arctic seas destroys the last of the polar bears' ice flows, how will their diets change? What are a few things the new food may do to their health?
A bacteriophage contains a single stranded DNA molecule. This DNA contains 19% adenine, 23% thymine, 45% guanine, and 13 percent cytosine. An RNA molecule complementary to this DNA is made.
Explain the possible fates of DNA that in brought into the recipient cell during generalized transduction.
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