What part of boil buffer helps break open bacterial cell

Assignment Help Biology
Reference no: EM1396692

1. What part of the Boil buffer helps "break open" the bacterial cells?

2. What is the difference between bacterial genomic DNA and plasmid DNA that results in the precipitation of genomic DNA with proteins, leaving the plasmid in the supernatant?

3. Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end "sticky" and why is this especially useful for inserting DNA fragments into plasmid DNA?

4. What specific property of DNA allows it to be seperated by size using a gel electrophoresis system?

Reference no: EM1396692

Questions Cloud

Determine standard deviation of the fruit length : Two extensively inbred strains of summer squash - designated "crookneck" and "scallop" - are crossed. The length of fruit in the crookneck averages 40 cm and that in the scallop averages 7 cm.
Diagram the alignment of these chromosomes during meiosis : Inversions are known to affect crossing over. The following homologs have the indicated gene order (the filled and open circles are homologous centromeres): filled ABCDE open ADCBE.
Dependent variables in regression analysis : Explain how we test independent against dependent variables in a regression analysis? What's the set up? How are regression analyses often misused / misinterpreted at work or in the news? Cite an example.
What is the common thread running through all of essays : List examples of things you disagreed with and why in Lewis Thomas Essays "The Lives of a Cell"
What part of boil buffer helps break open bacterial cell : Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end "sticky" and why is this especially useful for inserting DNA fragments into plasmid DNA?
Draw map of f+ strain indicating positions of all genes : Five Hfr stains A through E are derived from a single F+ strain of E. coli. The following chart shows the entry time of the first five markers into an F- strain
What mutagens can be used to revert mutants to wild type : What mutagens can be used to revert mutants to wild type with a hydroxylamine mutation?
Time series and forecasting : Victor Anderson, the owner of Anderson Belts, Inc., is studying absenteeism among his employees. His workforce is small, consisting of only five employees.
What does this have to do with lac- : Can someone explain replica plating in detail regarding xgal and xgal + iptg? I am studying for an exam and am very confused. I have in my notes something about mutations turn xgal +iptg blue when its supposed to be white and wild type should be x..

Reviews

Write a Review

Biology Questions & Answers

  Product of an independent fertilization event

The seeds in bush bean pods are each the product of an independent fertilization event. Green seed color is dominant to white seed color in bush beans.

  Differences in the kinds of dna sequences represented

Would you expect any differences in the sizes of the inserts in the two libraries? in brief explain.

  Drag each animal to the appropriate bin walrus

Animals can be classified according to their mode of thermoregulation. Sort the animals below, indicating their likely thermoregulatory strategy. Note that poikilotherms are sometimes referred to as heterotherms.

  The overall reaction of aerobic respiration of glucose

The overall reaction of aerobic respiration of glucose is summarized as.

  Question based on evolution

Several traits are affected by the environment, but also have a genetic component. How can we partition the sources of variation? Write a formula for this.

  Bacteria infect on cells

You start to have a scratchy feeling in the back of your throat. After a few days it hurts to swallow, so you go to doctor. The doctor tells you that you have strep throat.

  Forecast the frequency of yellow with white eyes

In Drosophila, the map directions of genes are given in map units numbering from one end of a chromosome to the other. The X chromosome of Drosophila is 66 m.u. long.

  Pcr to sequence a particular gene

Assume y ou wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volume.

  Find allele frequencies after one generation

Think about a population in which the frequency of allele A is p=0.7 and the frequency of allele a is q=0.3, and where the alleles are codominant.

  Warming of the arctic seas destroys

Suppose when warming of the Arctic seas destroys the last of the polar bears' ice flows, how will their diets change? What are a few things the new food may do to their health?

  Determine the value of found base

A bacteriophage contains a single stranded DNA molecule. This DNA contains 19% adenine, 23% thymine, 45% guanine, and 13 percent cytosine. An RNA molecule complementary to this DNA is made.

  Explain the possible fates of dna

Explain the possible fates of DNA that in brought into the recipient cell during generalized transduction.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd