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Q1. What are the principles and the purpose of using differential salt precipitation in protein purification?
Q2. 6-phosphogluconate dehydrogenase has a pI of 6. Explain why the buffer used for a chromatography on DEAE-cellulose must have a pH greater than 6 but less than 9 in order to ensure the enzyme is efficiently bound to the column?
Q3. We load a DEAE-cellulose column adjusted to a pH of 6.5 with the following mixture of proteins: ovalbumin (pI = 4.6), urease (pI = 5.0), and myoglobin (pI = 7.0). The proteins are eluted first with a buffer of weak ionic strength at pH of 6.5, and then the same buffer containing increasing amounts of sodium chloride is used to elute the proteins. What order are the proteins eluted?
Calculate the molarity of a solution made by dissolving 0.140 mol Na2SO4 in enough water to form exactly 800mL of solution.
Problem- Compound W, C8H17Br, and X, C8H17Cl, are products of the radical bromination (W), and chlorination (X), of 2,4-dimethylhexane. W and X are each chiral.
The thyroid horone thyroxine has the chenical formula C15H11Iodine4NO4. How many milligrams of C15H11Iodine4NO4 can be produced from 4.31x10^2 mg of iodine atoms, about twice the amount that a typical adult consumes in one day
A 250.0 -mL buffer solution initially contains 2.5X10^-2 M of HCHO2 and 2.5X10^-2 M of NaCHO2. What mass of the correct reagent should
find the number of representative particles in each given substance 1 3.00 mol sn2 0.400 mol kcl3 7.50 mol so24 4.80 x
What substitution product(s) is(are) obtained when 2-chlorobutane reacts with Ag/NO3/ethanol-water (1:1)
How many grams of sodium carbonate is formed from the reaction of 74.0g of sodium hydroxide and 44.0g CO2. How many grams of each material is present after the reaction
why during a GC is the boiling point for some samples higher than the final temperature used during the run yet these samples still run
Assuming that concentrated HNO3 (70% HNO3) is 16M, what is the molarity of a 1:1 HNO3:Water solution
Describe what happens in a fission reaction. What is a commonly used isotope in a nuclear fission reactor? Describe how a fission nuclear reactor power plant works.
The electrode acts as the anode, and the electrode acts as the cathode. To maintain electric neutrality, you add a salt bridge separating the two half-cells. What is the net cell reaction for the chromium-silver voltaic cell?
Consider the following equilibrium: Cu2+ + 4NH3 Cu(NH3)42+ (Kf = 4.8e+12) A solution is made by mixing 10.0 mL of 0.90 M CuSO4 and 1.50 L of 0.490 M NH3
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