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You are attempting to map the restriction sites for Ecor I and BamH I on a circular DNA molecule. After conducting single digests with Ecor I and BamH I as well as a double digest, you obtain the following data:
Ecor I BamH I Ecor I + BamH I
fragment 1 8000bp 2500 bp 1500 bpfragment 2 5500 bp 2500 bpfragment 3 4000 bp
What is the total length of the uncut DNA molecule?
How many restriction sites are there for BamH I?
Draw a diagram of the circular DNA molecule, showing the locations of the restriction sites for Ecor I and BamH I , as well as distances between them.
Illustrate the environmental conditions that would favour sexual reproduction over asexual reproduction in Hydra. What is the possible evolutionary reason for having two different reproductive strategies.
Your friend has now joined a lab that studies vesicle budding from the Golgi and has been given a cell line that does not form mature vesicles. He wants to begin designing some experiments but wasn't listening carefully when he was told about the mol..
State the key products of the Kreb's Cycle and how various of each there are starting from 1 glucose molecule.
What are the roles of COPI vesicles for transporting proteins between Golgi cisternae. How does a new Golgi cisterna form in the cis side from COPII vesicles.
A batch animal cell culture has a productivity of 1.8 g/L*day, with a MUmax=0.5 days, a 10% inoculums and a turnaround time of 5 hours. If a continuous culture could operate at a dilution rate of 90% MUmax, explain what would be the productivity.
If I had a biosphere of plants and animals and air and I radio labelled the carbon in co2, what path will that carbon take through the biosphere, where does carbon go.
A clinical microbiologist makes serial dilutions of several antimicrobials in broth, and then incubates each drug dilution series with a standard amount of a patient's isolated pathogen. What is this micro biologist setting up.
What aspects of the native organ function are replicated, and which are not? (this should include a quantitative analysis of function, for example filtration rates in artificial kidney compared to natural kidney)
Illustrate the metaphase cell of this culture showing predicted distribution of radioactivity on the autoradiograph.
suppose a single gene controls soybean seed color and that you already know the nucleotide sequence of the wild type allele.
Do you think biochemical testing must be recommended to detect the deleterious gene in Mrs. Lehman. Which one increased their alveolar ventilation, or do they stay the same? Why with appropriate reason.
Imagine that non disjunction occurred throughout the production of the egg cell that produced your dragon's baby and resulted in no sex chromosome in the sperm cell. How would this change phenotype of your dragons' baby? Be exact for each trait that ..
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