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The restriction enzyme ECOR I cuts DNA leaving 5' overhangs, Pst I leaves 3' overhangs after cutting DNA and SMA I leaves blunt ends on the fragments it produces.Indicate wheter DNA ligase can join the following pieces of DNA.
a) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was also cut with Ecor I .
b) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was cut with Pst I.
c) a fragment of human DNA that was generated by cutting with Sma I and a plasmid DNA that was also cut with Sma I.
You are a scientist working on a large protein (500 amino acids long) you have genetically engineered the protein in in such a way as to replace a single hydrophobic amino acid with a negatively charged amino acid.give the difference between prima..
Suppose after washing your hands, you leave ten bacterial cells on the new bar of soap. You next decide to do a plate count of the soap after it is left in the soap dish for 24 hours. You dilute 1g of the soap 1:10 and plate it on a standard plate co..
Distinguish between pathological and trauma. Explain the main pathways by which energy from the sun or from specific chemical reactions enters organisms and passes from organism to organism or back into the environment.
Birds and mammals have a four-chambered heart, with two ventricles and two atria, but other modern reptiles have a three-chambered heart, with just one ventricle. Paleontologists debate whether dinosaurs had a typical "reptile-like" heart or a "birdl..
How several bacteria were on the soap? How did they get there. Unlike squamous cells, cuboidal and columnar cells have large, open cytoplasms. Which functions of epithelial tissue are supported by having such big cells.
If you transform bacteria with the plasmid library made in part b above, divide the transformatnts into 20 aliquots and find one has the insert of interest, now how many clones do you need to screen.
Complex, Simple and Hypercomplex (Endstop cells) cells. Layer 4C and synapse onto simple cells. Whether or not they aer bars of light Simple cell is listening to a group of LGN cells. So each increment of 2.5 degrees has different simple cells.
Her breath smells like acetone and her blood glucose test out at 650mg/100ml of blood. She is in acidosis. What hormone must be administered to her and why. Where may she find such DNA.
What specific biotechnology application did you choose. What benefits are derived from this application.
Represent an arrow pointing away from each circle that represents food for another circle - and point the arrow towards the "eater's" circle. Some circles will have several arrows pointing towards hem and away from them. Illustrate an X in the circle..
I am conducting an activity assay to assess the Enzymatic Hydrolysis of 4-Nitrophenyl ?-D-Glucopyranoside by ?-D-Glucosidase. I am happy with the assay, though one of the components is bovine serum albumin ( 0.2% solution in phosphate buffer, to w..
t is well known that rubbing a minor wound inhibits the sensation of pain that an individual is experience. Please design a circuit that would explain this phenomenon (i.e., how would you put together a set of neurons in the spinal cord or beyond to ..
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