What is the specific activity

Reference no: EM1398117

You have measured the absorption of pNP created by acid phosphatase after 12.5 minutes of reaction to be 0.188. The reaction occurred in a 500 microliter volume into which was added 0.200mL of 0.400 mg/mL WAP. The reaction was stopped with the addition of 2.50 mL Na carbonate. The molar absorptivity of pNP is 1.88 x 10^4 L/(mole cm) and the light path length is 1.00 cm.

What is the specific activity?

Can you please be very detailed with all equations and explanations?

Reference no: EM1398117

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