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Angie, a 15 yr old female, was admitted to the hospital because of recurrent episodes of pallor, jaundice, and an ulcer on her left leg. Diagnosis of hemolytic anemia had been made at the age of 3 months, and she had been transfused several times since the diagnosis. Physical examination showed jaundice, skeletal growth retardation, and enlargement of the spleen. An analysis of blood showed low Hb content, low RBC count, and elevated reticulocyte count. Morphologic analysis revealed a mixed population of RBCs containing some irregularly contracted cells with irregular surface projections. The life span of the RBCs was found to be severely shortened. Both total and indirect serum bilirubin levels were markedly elevated. Fecal urobilinogen excretion was increased. Biochemical analysis of RBC metabolites showed elevated levels of 2,3 bisphosphoglycerate (2,3 BPG) and reduced levels of ATP. A splenectomy was performed; the spleen showed congestion and the presence of hemosiderin granules. Following splenectomy, there was improvement in both the clinical and hematologic symptoms. An increase in hemoglobin concentration as well as in the number of RBCs and reticulocytes was observed. The biochemical composition of the RBCs did not change after splenectomy
What is the problem with this patient and what is the source of ATP in red blood cells?
when chicken egg whites( which are chiefly composed of proteins) are heated they harden although we cannot reverse this process, we can dissolve the hardened egg white if we heat it in a solution containing a strong detergent.
Do you think that using recombinant DNA technologies to change the genetic composition of human egg cell is ever justified? If so, what restrictions have to be placed on such a use.
If we injected 3X hypertonic sucrose to a rabbit. What should the result be?
What would likely be the result of heightened inbreeding in a small population; increase the frequency of homozygous genotypes, change the frequency of the alleles, increase the frequency of dominant alleles, increase the frequency of heterozygote..
Researchers have adapted the principles of affinity chromatography to purify different types of protein, including lectin, recombinant protein and antibodies. Describe the ligand and matrix associated with isolating each of these three proteins.
In a donor population, the allele frequencies for the normal and the sickle-cell allele are 0.9 and 0.1 respectively. A group of 55 individuals migrates to population containing 10,000 individuals.
What is the complementary RNA strand that is created. Assuming this sequence is within an exon and in-frame, what would be the polypeptide formed from it?
Three genes in the soybean plant, Glycine max, are being studied for their linkage relationship. The genes are as given:
While discussing their findings, authors have ignored a main threat to the validity of their estimates of the exposure. Explain and discuss this error.
Determine chromosomal condition results in an organism being only half as fertile as a wild type individual of the same species?
Say you injected and x amount of protein onto SEC column and it produced certain peaks. Now say you repeated the experiment and use the same amount of protein.
Huntington disease is an autosomal dominant trait that causes neurodegeneration later in life. Matt, who is too young to show the phenotype of Huntington disease,
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