What is the g+c of the above oligonucleotide

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1) Due to the generosity of your lab mate, you are able to perform standard PCR to amplify your gene and you need to design primers. The native carboxyl terminus of your gene is encoded by the sequence 5'- CTAAAGTTTAAAGTAG-3'. You are reminded that you need to add a BamHI (5'-GGATCC-3') site to your reverse PCR primer to aid downstream cloning. Report the 5' to 3' sequence of the synthetic oligonucleotide that anneals to this end of the gene AND incorporates a BamHI site directly adjacent to the stop codon.

2) What is the G+C% of the above oligonucleotide?

Reference no: EM132439551

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