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What is the catalytic efficiency of fumarase

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Q1) The citric acid cycle enzyme, fumarase, catalyzes the conversion of fumarate to malate:

Fumarate + H2O à Malate

The turnover number, kcat, for fumarase is 800/sec. The Km of fumarase for its substrate is 5uM.

(a) In an experiment using 2 nM of fumarase, what is Vmax?

(b) The cellular concentration of fumarate is 47.5 uM. What is V0 when [fumarate] = 47.5 uM?

(c) What is the catalytic efficiency of fumarase?

Reference no: EM131738651

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