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You would like to genetically engineer a bacteria so it will glow in the presence of an environmental contaminant. The bacteria you select has the ability to grow in the presence of the contamination and secretes a specific enzyme in response to the contamination. You decide to use a fusion protein based system.
What is a fusion protein and how would you construct it.
Once you have your fusion protein you will need to insert into a plasmid which will serve as the reporter mechanism. Describe how you would construct this plasmid (describe the promotors, binding sites not the actual molecular techniques used to construct the plasmid) in order make the bacteria glow in the presence of contamination.
the I2 sublimes and gasses iodine heptofluride forms 3.2x10^2 torr of T2 and 4.1 g of solid I2 are put in to a 2.5 L Container at 2.5x10^2 K and container is heated to 5.5 x 10^2 K A what is the final pressure in atms. what is the partial pressure..
Fish produce glycerol to survive the freezing waters of the Arctic. Determine the degree to which the freezing point of blood and other biological fluids is lowered with the help of glycerol. What is the ideal concentration range of glycerol requi..
what are the [H3O^+] and OH^-] for a solution with each of the following pH values? a)10.0 b)5.0 c)6.5 d)1.82
What is the heat capacity of a bomb calorimeter if the combustion of 2.757 mmol of salicylic acid (C7H6O3(s), Hcomb = -3022 kJ/mol) increases the temperature of the calorimeter by 2.612°C
A particulate water sample that is saturated in CaF2 has a Ca2+ content of 105 ppm (that is, 105 g Ca2+ per 10^6 g of water sample).
At what velocity (m/s) must a 15.9 g object be moving in order to possess a kinetic energy of 1.0J?
In doing the Calorimetry experiment, a student did not follow the directions exactly. How would the experiment be affected if no plot of temperature was carried out. Instead the student used the value of the water just after mixing with the metal ..
How much energy is required to vaporize 158g of butane (C4H10) at its boiling point, if it has Hvap is 24.3kJ/mil
Calculate the sodium ion concentration in a solution formed by diluting 35 ml of a .585 M sodium phosphate solution to a final volume of 145ml
Pd has a FCC lattice in which hydrogen atoms occupy octahedral holes. If 70% of the holes are filled by hydrogen atoms and the lattice does not expand upon hydrogenation, how many grams of hydrogen will be contained in one cubic centimeter of the ..
97.5g sodium chloride were dissolved in distilled water in 500ml volumeristic flask. the mixture was stirred to form a
A 8.45-L container holds a mixture of two gases at 33 °C. The partial pressures of gas A and gas B, respectively, are 0.415atm and 0.606 atm. If 0.190 mol of a third gas is added with no change in volume or temperature, explain what will the total..
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