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Volumetric flask and diluting to the mark with water

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Reference no: EM132412945

Your intern spills the entire bottle of 0.1 M, pH 5.00 buffer that came with an enzymatic assay kit for malic acid. Rather than buying a new kit, you ask your intern to make an acetic acid/acetate buffer with this pH. Your intern starts by adding 2.86 mL acetic acid and 4.10 g sodium acetate to a 1.00 L volumetric flask and diluting to the mark with water.

Acetic acid: pKa 4.76, density 1.05 g/mL

a. Conjugate acid and conjugate base combined, does the overall buffer concentration (M) match the buffer provided with the kit?

b. What volume (mL) of 1 N NaOH is required to adjust the pH to the desired 5.0?

Reference no: EM132412945

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Volumetric flask and diluting to the mark with water : Your intern starts by adding 2.86 mL acetic acid and 4.10 g sodium acetate to a 1.00 L volumetric flask and diluting to the mark with water.

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Volumetric flask and diluting to the mark with water

Reference no: EM132412945

Reference no: EM132412945

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