Utilize the results from replica-plating to reply

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Q1. How would you test for involvement of the deep peroneal nerve, keep in mind that dorsiflexion of foot and toes might be severely interfered with by anoxia (lack of oxygen) of the muscles in the compartment, and that loss of muscle action therefore does not essentially imply nerve involvement?

Q2. Conjugation mapping can be used to locate genes on a bacterial chromosome. In this example, the F+ strain is able to synthesize methionine, valine, leucine, and histidine. The F- strain is unable to synthesize these amino acids; therefore, they must be supplied in the growth medium. Utilize the results from replica-plating to reply the questions.

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