Two-hybrid screens to test your hypothesis

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You are investigating the role of Gene M in motor neurons. Through coimmunoprecipitation, ChlP seq, and mass spectrometry, you have identified three proteins (A, B, and C) that seem to associate with your promoter and each other in a cell culture model of motor neurons. You suspect, however, that one of these interactions is an experimental artifact, and only two of the proteins associate under cytosolic conditions.

Design a series of two-hybrid screens to test your hypothesis (drawings may make this easier) Think about the selection conditions you need in order to know you have the two plasmids of interest, as well as something else to act as a reporter for interactions between two interacting proteins.

Reference no: EM133307432

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