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A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then aneealed with PstI digest of human DNA, ligated and used to transform E.Coli cells. a) what antibiotic-resistance phenotypes will be found on the plate b) what antibiotic woiuld you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid withou an insert? c) which phenotype will indicate the presence of plasmids that contain human DNA fragments?
A 757 mL gas sample at STP is compressed toa volume of 202 mL, and the temperature isincreased to 30 degrees C. What is the new pressureof the gas in Pa?
Calculate the final temperature of the helium inside the cylinder. Assume helium to be an ideal gas with Cp=(5/2)R.
The normal melting and boiling points of O2 are -218C and -183C respectively. Its triple point is at -219C and 1.14 torr, and its critical point is at -119C and 49.8 atm.
The vapor pressure of a liquid is the pressure exerted by its vapor when the liquid and vapor states are in equilibrium. The relationship between vapor pressure and temperature is expressed by the Clausius-Clapeyron equation.
What colume will 500 mL of gas at 20 degrees celsius and a pressure of 420 mm Hg occupy if the temperature is reduced to -80 degrees celsius
What is the pressure in atmospheres inside a container of gas connected to a mercury-filled open-end manometer when the level in the arm connected to the container is 21.2cm higher than the level in the arm open
It takes 49.0 J to raise the temperature of an 11.7 g piece of unknown metal from 13.0 C to 24.0 C. What is the specific heat for the metal?
Identify the major type of interactive force of MgF2? how do you find the major type of interactive force?
In the acid-base reaction between Sr(OH)2 and HNO3, 0.75 L of 0.85 M Sr(NO3)2 is formed upon the reaction of 0.515 L of HNO3 solution and excess Sr(OH)2.
Calculate the pH of the resultant mixtures: a. 10 ml of 0.2 M Ca(OH)2 + 25 ml of 0.1 M HCL b. 10 ml of 0.01 M H2SO4 + 10 ml of 0.01 M Ca(OH)2 c. 10 ml of 0.1 M H2SO4 + 10 ml of 0.1 M KOH
hydrochloric acid was added to a 50.00 mL solution containing your sodium carbonate unknown. calculate the pH of the solution at the second equivalence point.
What is the pH of the solution resulting from the addition of 25.0 mL of .01M NaOH solution to 40.0 mL of .01M acetic acid
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