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What might be the most useful in determining the amino acid residues of an enzyme catalyzed reaction?
a. Unfold the enzyme with urea and 2-mercaptoethanol. Then incubate and crystallize it in the presence of the substrate followed by X-ray crystallography.
b. Use an irreversible inhibitor that reacts with the side chains of specific amino acids.
c. Determine the entire primary structure of the protein.
d. Use a competitive inhibitor and plot the rate vs concentration of inhibitor.
A solution containing 100g of unknown liquid and 900g water has a freezing point of -3.33C. Given Kf=1.86C/m for water, determine the molecular weight of the unknown liquid and express your answer in g/mol.
Aluminum hydroxide and sulfuric acid reaction, Which reagent is the limiting reactant when 0.450 mol Al(OH)3 and 0.550 mol H2SO4 are allowed to react? How many moles of the excess reactant remain after the completion of the reaction? Please explain..
For the following reactions, identify the type (combination, combustion, decomposition, displacement, methathesis [double-displacement)] neutralization), balance the reaction, and for the redox reactions identify the oxidizing agent and reducing a..
A total of 0.155 of this subunit was dissolved in enough water to produce 2.00 of solution. At 28 the osmotic pressure produced by the solution was 0.138 . What is the molar mass of the protein?
Many organic chemists when making a new solid compound often first try ethanol as a recrystallizing solvent. If soluable at room temperature, they simply add water and this often works! Why?
Aluminum will react with bromine to form aluminum bromide (Al2Br6). How many moles of Al are needed to form 28.0 mol of Al2Br6?
A physician treating a patient with megaloblastic anemia might be tempted to treat the patient with folic acid to see if a simple folate deficieny is involved.
A 4.90 g nugget of pure gold absorbed 266 J of heat. What was the final temperature of the gold if the initial temperature was 23.0°C? The specific heat of gold is 0.129 J/(g·°C).
3H2 (g) + N2 (g) 2NH3(g), Kc = 6.0 × 10-2 at 500°C for this nitrogen fixation reaction. What is the equilibrium concentration of N2 If 0.250 M H2 and 0.050 M NH3 are present at equilibrium?
A 17.0-g sample of ice at -19.0°C is mixed with 101.0 g of water at 87.0°C. Calculate the final temperature of the mixture assuming no heat loss to the surroundings.
ammonium hydrogen phosphate is made from reacting phosphoric acid with ammonia. if 10.00g of ammonia react, how many moles of fertilizer will be produced?
the osmotic pressure of this solution was 0.00222 atm at 25 C. calculate the molar mass of hemoglobin, which is a molecular compound and a nonelectrolyte.
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