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The data in the table at the right show a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and intercept of the best straight line fit to the calibration curve. (Do not subtract the blank reading when creating your calibration curve.)
RNA (ug) Absorbance at 260nm
0.00 0.299
10.43 0.556
20.86 0.823
31.29 1.187
41.72 1.396
Slope=0.27
intercept=.2872
Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.690.
mass RNA= _____ug
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