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Hydrolytic driving force. The hydrolysis of pyrophosphate to orthophosphate is important in driving forwards biosynthetic reactions such as the synthesis of DNA. THis hydrolytic reaction is catalyzed in E. coli by a pyrophosphatase that has a mass of 120 kd and consists of 6 identical subunits. For this enzyme, a unit of activity is defined as the amount of enzyme that hydrolyzes 10µmol of pyrophosphate in 15min at 37degrees celsius under std assay conditions. The purified enzyme has a Vmax of 2800 units per milligram of enzyme.
(a)How many moles of substrate are hydrolyzed persecond per milligram of enzyme when the substrate conc is much greater than KM?
(b)How many moles of active sites are there in 1mg of enzyme?
(c)What's the turnover number of the enzyme?
A mating is made between two individuals who are first cousins and individual X is produced from this mating. Individual E is a parent of S and individual F is a parent of D.
what are the two types of lymphocytes and where are they made and where are they located in the body once mature?
Provide advice to someone traveling in a third world country as to what foods and drinks it would be wise to avoid and as to which food preparation methods might make foods safe to eat or drink to avoid bacteria.
Describe the potential legal and ethical issues associated with establishing new wild populations.
What factors are considered in establishing recommended protein intakes?
The closest living relatives to modern humans are: a) promisians, b)african apes, c)new world monkeys, d)asian apes, e)old world monkeys.
In the United States, one out of approximately 10,000 babies is born with PKU. Assuming this trait is in Hardy-Weinberg equilibrium, approximately what percent of the total population are heterozygous carriers of the recessive (little 'a') PKU all..
Why would reading something for a long time cause eye fatigue, while looking at something distant is restful?
Name an example of a red blood cell membrane protein that carries out each function of that you gave in part (a) of this question.
For vaccines to be beneficial, the vaccine antigens must activate the immune system to generate memory cells. Describe the properties of memory B and T cells that make them more effective in responding to the infection.
What effects are produced by an enzyme on the general reaction below? The rate constant for the forward reaction (K1) increases
What is the probability of each of the above arrangements occurring? (v) Given that Julie is a carrier, which of the above arrangements must be correct?
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