Rotenone treated mitochondria affect on oxygen consumption

Reference no: EM1396075

Rotonone inhibits Complex I Cyanide inhibits Complex IV Oligomycin inhibits ATP synthase You incubate isolated intact mitochondria in a buffered solution containing NADH, ADP plus Pi. Oxygen is consumed and ATP is made.

1. Upon adding cyanide you examine the effect on oxygen consumption and the production of ATP. After a period of time has passed what will be the effect on: a) oxygen consumption b) ATP synthesis

2. You add the inhibitor rotenone to the respiring mitochondrial preparation. After a period of time has passed, what will be the effect on a) oxygen consumption b) ATP synthesis

3. You add succinate to the rotenone treated mitochondria. After a period of time has passed, what will be the effect on c) oxygen consumption d) ATP synthesis Explain each effect you predict.

Reference no: EM1396075

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