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Question: You are performing a reverse transcription (RT) reactions for high-throughput analysis using a 96-well plate format. Each 96-well plate is 8 horizontal rows of wells by 12 vertical columns of wells. You need to set up the entire 96-well plate will identical RT components except for the particular primers that you will be using. Each of the 8 columns on the plate will get it's own unique primer pair. The best way to accomplish this is to produce a 1 giant mastermix containing all the necessary components minus the primers, and then aliquot the primers as needed into the 8 columns. RT reactions require the following components: buffer, template RNA, dNTPs, RT polymerase, primers, and water. The final volume of each RT reaction is 25 ul.Reagents available:2X RT Buffer50 uM dNTPs537 ug/ul RNA1.0 units/ul RNA polymerasePrimer mix at 100 uMSterile waterBased on your protocol, each RT reaction requires: 1X RT buffer, 5 uM dNTPs, 50 ng/ul RNA, 0.5 units of RT polymerase, and 10 uM each primer.(A)How much of each component will you need for 1 RT reaction? Show your work(B)How much of each component will you need for a mastermix for all 96 reactions? Show your work.
Describe what are the three different microbial groups in anaerobic digester, and how they work together to convert complex polymers to methane gas
Discuss how it is that directional selection on quantitative traits can produce populations with trait distributions beyond what any parental generation had even without any new mutations creating new alleles.
Compute the frequencies of the genotypes in future generations if the initial population consists of the following genotype frequencies.
Work out this chi-square value. How to do a Chi Square trouble for the gentics class.
Discuss why is even weak selection still stronger than drift in very large populations at non-neutral loci?
DNA sequences are used quite often to evolutionary relationships between species. Explain why is the DNA sequence of one gene a less accurate indicator of the evolutionary.
Explain what happens during each of the three main processes involved in the production of urine, namely filtration, reabsorption, and secretion.
Assumeou are investigating four recessive alleles in corn. They are whimpy ears ( w ), striped leaves ( s ), shriveled kernels ( k ), and red tassels ( r ). Their dominant counterparts are represented
A new drug targets renal function through changing ion transport from the filtrate to the interstitial fluid. This particular medicine blocks active transport of sodium in the nephrons.
The given statements describe SDS-PAGE, a method used to separate proteins. Some of the statements are correct, and some of them are incorrect.
When cloning a gene into an expression vector, people frequently use two different restriction enzymes so that passenger DNA can be inserted in the correct orientation with respect to promoter sequence present in the plasmid. But orientation is almos..
What could be expected phenotypes, and in what number will the y be expected? Suppose no linkage.
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