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In studying receptor-ligand interactions, the researcher wanted to narrow down the exact region of binding between the receptor and the ligand. Amino acids 24 - 29 on the receptor were believed to be involved in ligand binding. So the researcher performed mutations in these amino acids and assayed the effect of these mutations by performing binding assays.
Mutations Receptor-Ligand binding
Amino acid 24 No
Amino acid 25 Yes
Amino acid 26 No
Amino acid 27 Yes
Amino acid 28 No
Amino acid 29 Yes
Double mutant Amino acids 24 and 25 Yes
From these experiments.....
What did the researcher learn from mutation in amino acid 24?
What did the researcher learn from the double mutant 24 and 25 ?
Which of the amino acids are involved in receptor-ligand binding? Explain.
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